The effect of hepatopancreas homogenate of the Red king crab on HA-based filler

• Published in: PeerJ
• Main Authors: Tatyana Ponomareva, Dmitrii Sliadovskii, Maria Timchenko, Maxim Molchanov, Alexander Timchenko, Evgeny Sogorin
• Format: Article in Journal/Newspaper
• Language: English
• Published: PeerJ Inc. 2020
• Subjects: Treating filler complications; Hyaluronidase; Red king crab; Hepatopancreas; Hyaluronic acid; Hyaluronidase activity; Medicine; R; Biology (General); QH301-705.5
• Online Access: https://doi.org/10.7717/peerj.8579; https://doaj.org/article/4f5f687cb7e84fad9b076907f8ecb448

In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications.