Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman
Objective: To perform phytochemical screening and to evaluate the in-vitro antioxidant and anti-inflammatory activities of ethanolic extract of seeds and fruits of Zizyphus spina-christi (ZSC) growing in Oman. Methods: Alcoholic extract of the dry powdered seeds and fruits of ZSC was obtained by col...
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Wolters Kluwer Medknow Publications
2014
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ftdoajarticles:oai:doaj.org/article:4f5253ea9637490aa888b82e1481d1c3 2023-05-15T15:08:23+02:00 Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman Fatma Alhakmani Shah Alam Khan Aftab Ahmad 2014-07-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0273 https://doaj.org/article/4f5253ea9637490aa888b82e1481d1c3 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115300666 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0273 https://doaj.org/article/4f5253ea9637490aa888b82e1481d1c3 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S2, Pp S656-S660 (2014) Antioxidant Anti-inflammatory activity DPPH Total phenolic content Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0273 2022-12-31T04:04:04Z Objective: To perform phytochemical screening and to evaluate the in-vitro antioxidant and anti-inflammatory activities of ethanolic extract of seeds and fruits of Zizyphus spina-christi (ZSC) growing in Oman. Methods: Alcoholic extract of the dry powdered seeds and fruits of ZSC was obtained by cold maceration method and was subjected to preliminary phytochemical screening. Total phenolic content were estimated by using Folin-Ciocalteu reagent and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to determine in-vitro antioxidant activity of plant extracts. Anti-inflammatory activity was investigated by protein denaturation method. Results: Phytochemical analysis of both the extracts revealed the presence of major classes of phytochemicals such as tannins, alkaloids, flavonoids, cardiac glycosides etc. ZSC seeds were found to contain the highest total phenolics but ZSC fruits exhibited the maximum antioxidant activity. The anti-inflammatory activity of both parts of the plant extract was significant and comparable with the standard anti-inflammatory drug, diclofenac. Conclusions: Based on the results of this pilot study, it can be concluded that ZSC is a good source of natural antioxidants which can be used to prevent progression of many chronic diseases. Further detailed phytochemical studies are needed to identify the chemical compounds responsible for exhibiting potent anti-inflammatory activity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S656 S660 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antioxidant Anti-inflammatory activity DPPH Total phenolic content Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Antioxidant Anti-inflammatory activity DPPH Total phenolic content Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Fatma Alhakmani Shah Alam Khan Aftab Ahmad Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman |
topic_facet |
Antioxidant Anti-inflammatory activity DPPH Total phenolic content Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To perform phytochemical screening and to evaluate the in-vitro antioxidant and anti-inflammatory activities of ethanolic extract of seeds and fruits of Zizyphus spina-christi (ZSC) growing in Oman. Methods: Alcoholic extract of the dry powdered seeds and fruits of ZSC was obtained by cold maceration method and was subjected to preliminary phytochemical screening. Total phenolic content were estimated by using Folin-Ciocalteu reagent and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to determine in-vitro antioxidant activity of plant extracts. Anti-inflammatory activity was investigated by protein denaturation method. Results: Phytochemical analysis of both the extracts revealed the presence of major classes of phytochemicals such as tannins, alkaloids, flavonoids, cardiac glycosides etc. ZSC seeds were found to contain the highest total phenolics but ZSC fruits exhibited the maximum antioxidant activity. The anti-inflammatory activity of both parts of the plant extract was significant and comparable with the standard anti-inflammatory drug, diclofenac. Conclusions: Based on the results of this pilot study, it can be concluded that ZSC is a good source of natural antioxidants which can be used to prevent progression of many chronic diseases. Further detailed phytochemical studies are needed to identify the chemical compounds responsible for exhibiting potent anti-inflammatory activity. |
format |
Article in Journal/Newspaper |
author |
Fatma Alhakmani Shah Alam Khan Aftab Ahmad |
author_facet |
Fatma Alhakmani Shah Alam Khan Aftab Ahmad |
author_sort |
Fatma Alhakmani |
title |
Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman |
title_short |
Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman |
title_full |
Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman |
title_fullStr |
Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman |
title_full_unstemmed |
Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman |
title_sort |
determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of zizyphus spina-christi grown in oman |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0273 https://doaj.org/article/4f5253ea9637490aa888b82e1481d1c3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S2, Pp S656-S660 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115300666 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0273 https://doaj.org/article/4f5253ea9637490aa888b82e1481d1c3 |
op_doi |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0273 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S656 |
op_container_end_page |
S660 |
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1766339760967647232 |