Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom
Abstract Background The yellow scorpion Tityus serrulatus (Ts) is responsible for the highest number of accidents and the most severe scorpion envenoming in Brazil. Although its venom has been studied since the 1950s, it presents a number of orphan peptides that have not been studied so far. The obj...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:4f2ef57cca4f4f5daa4a434d2097e9b8 2023-05-15T15:19:15+02:00 Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom Priscila C. Lima Karla C. F. Bordon Manuela B. Pucca Felipe A. Cerni Karina F. Zoccal Lucia H. Faccioli Eliane C. Arantes 2015-12-01T00:00:00Z https://doi.org/10.1186/s40409-015-0051-6 https://doaj.org/article/4f2ef57cca4f4f5daa4a434d2097e9b8 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100353&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0051-6 https://doaj.org/article/4f2ef57cca4f4f5daa4a434d2097e9b8 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) β-KTx cytokine interleukin neurotoxin pro-inflammatory scorpion venom Tityus serrulatus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2015 ftdoajarticles https://doi.org/10.1186/s40409-015-0051-6 2022-12-31T11:32:04Z Abstract Background The yellow scorpion Tityus serrulatus (Ts) is responsible for the highest number of accidents and the most severe scorpion envenoming in Brazil. Although its venom has been studied since the 1950s, it presents a number of orphan peptides that have not been studied so far. The objective of our research was to isolate and identify the components present in the fractions VIIIA and VIIIB of Ts venom, in order to search for a novel toxin. The major isolated toxins were further investigated for macrophage modulation. Methods The fractions VIIIA and VIIIB, obtained from Ts venom cation exchange chromatography, were rechromatographed on a C18 column (4.6 × 250 mm) followed by a reversed-phase chromatography using another C18 column (2.1 × 250 mm). The main eluted peaks were analyzed by MALDI-TOF and Edman’s degradation and tested on macrophages. Results The previously described toxins Ts2, Ts3-KS, Ts4, Ts8, Ts8 propeptide, Ts19 Frag-II and the novel peptide Ts19 Frag-I were isolated from the fractions VIIIA and VIIIB. Ts19 Frag-I, presenting 58 amino acid residues, a mass of 6,575 Da and a theoretical pI of 8.57, shares high sequence identity with potassium channel toxins (KTx). The toxins Ts4, Ts3-KS and the partially purified Ts19 Frag-I did not produce cytotoxic effects on macrophage murine cells line (J774.1). On the other hand, Ts19 Frag-I induced the release of nitric oxide (NO) by macrophages, while Ts4 and Ts3-KS did not affect the NO production at the tested concentration (50 μg/mL). At the same concentration, Ts19 Frag-I and Ts3-KS increased the production of interleukin-6 (IL-6). Ts19 Frag-I and Ts4 did not induce the release of IL-10, IL-1β or tumor necrosis factor-α by macrophage cells using the tested concentration (50 μg/mL). Conclusions We partially purified and determined the complete sequence and chemical/physical parameters of a new β-KTx, denominated Ts19 Frag-I. The toxins Ts4, Ts3-KS and Ts19 Frag-I showed no cytotoxicity toward macrophages and induced IL-6 release. Ts19 Frag-I ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 21 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
β-KTx cytokine interleukin neurotoxin pro-inflammatory scorpion venom Tityus serrulatus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
β-KTx cytokine interleukin neurotoxin pro-inflammatory scorpion venom Tityus serrulatus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Priscila C. Lima Karla C. F. Bordon Manuela B. Pucca Felipe A. Cerni Karina F. Zoccal Lucia H. Faccioli Eliane C. Arantes Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom |
topic_facet |
β-KTx cytokine interleukin neurotoxin pro-inflammatory scorpion venom Tityus serrulatus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background The yellow scorpion Tityus serrulatus (Ts) is responsible for the highest number of accidents and the most severe scorpion envenoming in Brazil. Although its venom has been studied since the 1950s, it presents a number of orphan peptides that have not been studied so far. The objective of our research was to isolate and identify the components present in the fractions VIIIA and VIIIB of Ts venom, in order to search for a novel toxin. The major isolated toxins were further investigated for macrophage modulation. Methods The fractions VIIIA and VIIIB, obtained from Ts venom cation exchange chromatography, were rechromatographed on a C18 column (4.6 × 250 mm) followed by a reversed-phase chromatography using another C18 column (2.1 × 250 mm). The main eluted peaks were analyzed by MALDI-TOF and Edman’s degradation and tested on macrophages. Results The previously described toxins Ts2, Ts3-KS, Ts4, Ts8, Ts8 propeptide, Ts19 Frag-II and the novel peptide Ts19 Frag-I were isolated from the fractions VIIIA and VIIIB. Ts19 Frag-I, presenting 58 amino acid residues, a mass of 6,575 Da and a theoretical pI of 8.57, shares high sequence identity with potassium channel toxins (KTx). The toxins Ts4, Ts3-KS and the partially purified Ts19 Frag-I did not produce cytotoxic effects on macrophage murine cells line (J774.1). On the other hand, Ts19 Frag-I induced the release of nitric oxide (NO) by macrophages, while Ts4 and Ts3-KS did not affect the NO production at the tested concentration (50 μg/mL). At the same concentration, Ts19 Frag-I and Ts3-KS increased the production of interleukin-6 (IL-6). Ts19 Frag-I and Ts4 did not induce the release of IL-10, IL-1β or tumor necrosis factor-α by macrophage cells using the tested concentration (50 μg/mL). Conclusions We partially purified and determined the complete sequence and chemical/physical parameters of a new β-KTx, denominated Ts19 Frag-I. The toxins Ts4, Ts3-KS and Ts19 Frag-I showed no cytotoxicity toward macrophages and induced IL-6 release. Ts19 Frag-I ... |
format |
Article in Journal/Newspaper |
author |
Priscila C. Lima Karla C. F. Bordon Manuela B. Pucca Felipe A. Cerni Karina F. Zoccal Lucia H. Faccioli Eliane C. Arantes |
author_facet |
Priscila C. Lima Karla C. F. Bordon Manuela B. Pucca Felipe A. Cerni Karina F. Zoccal Lucia H. Faccioli Eliane C. Arantes |
author_sort |
Priscila C. Lima |
title |
Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom |
title_short |
Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom |
title_full |
Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom |
title_fullStr |
Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom |
title_full_unstemmed |
Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom |
title_sort |
partial purification and functional characterization of ts19 frag-i, a novel toxin from tityus serrulatus scorpion venom |
publisher |
SciELO |
publishDate |
2015 |
url |
https://doi.org/10.1186/s40409-015-0051-6 https://doaj.org/article/4f2ef57cca4f4f5daa4a434d2097e9b8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100353&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0051-6 https://doaj.org/article/4f2ef57cca4f4f5daa4a434d2097e9b8 |
op_doi |
https://doi.org/10.1186/s40409-015-0051-6 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
21 |
container_issue |
1 |
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1766349440056033280 |