Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis.
Biomarkers to predict the severity of leptospirosis are still lacking. This study aimed to identify and validate microRNAs in patients with severe leptospirosis, that could potentially be used as biomarkers for predicting an unfavorable outcome. Serum samples were collected from participants with de...
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ftdoajarticles:oai:doaj.org/article:4f0faa2918564daeb3c0323b26652704 2023-05-15T15:09:50+02:00 Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis. Umaporn Limothai Janejira Dinhuzen Titipon Payongsri Sasipha Tachaboon Pisit Tangkijvanich Natthaya Chuaypen Nattachai Srisawat 2020-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008809 https://doaj.org/article/4f0faa2918564daeb3c0323b26652704 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008809 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008809 https://doaj.org/article/4f0faa2918564daeb3c0323b26652704 PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008809 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008809 2022-12-31T07:08:54Z Biomarkers to predict the severity of leptospirosis are still lacking. This study aimed to identify and validate microRNAs in patients with severe leptospirosis, that could potentially be used as biomarkers for predicting an unfavorable outcome. Serum samples were collected from participants with definite diagnosis of leptospirosis. The participants were divided into two groups, non-severe and severe leptospirosis, as defined by the Specific Organ Sequential Organ Failure (SOFA) Score of more than two in any organ. Microtranscriptome analysis was performed using the NanoString miRNA Expression Assay. The expression level of candidate miRNAs was then validated by quantitative RT-PCR. Based on the NanoString, the microtranscriptome profile of the severe group was significantly different from that of the non-severe group. Upregulation of miR155-5p, miR362-3p, miR502-5p, miR601, miR1323, and miR630 in the severe group were identified, and further investigated. A total of 119 participants were enrolled in the validation cohort. Serum miR155-5p and miR630 levels were significantly higher in the severe group compared to the non-severe group. The combined use of miR155-5p or miR-630 with serum bicarbonate levels had an AUC of 0.79 (95%CI; 0.69-0.89, p<0.001) in identifying the severity of the disease. This data provides the first evidence that the microtranscriptome profiles of patients with severe leptospirosis were different from the non-severe group. Serum miR155-5p and miR630 levels might be novel biomarkers for identifying severe leptospirosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 11 e0008809 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Umaporn Limothai Janejira Dinhuzen Titipon Payongsri Sasipha Tachaboon Pisit Tangkijvanich Natthaya Chuaypen Nattachai Srisawat Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Biomarkers to predict the severity of leptospirosis are still lacking. This study aimed to identify and validate microRNAs in patients with severe leptospirosis, that could potentially be used as biomarkers for predicting an unfavorable outcome. Serum samples were collected from participants with definite diagnosis of leptospirosis. The participants were divided into two groups, non-severe and severe leptospirosis, as defined by the Specific Organ Sequential Organ Failure (SOFA) Score of more than two in any organ. Microtranscriptome analysis was performed using the NanoString miRNA Expression Assay. The expression level of candidate miRNAs was then validated by quantitative RT-PCR. Based on the NanoString, the microtranscriptome profile of the severe group was significantly different from that of the non-severe group. Upregulation of miR155-5p, miR362-3p, miR502-5p, miR601, miR1323, and miR630 in the severe group were identified, and further investigated. A total of 119 participants were enrolled in the validation cohort. Serum miR155-5p and miR630 levels were significantly higher in the severe group compared to the non-severe group. The combined use of miR155-5p or miR-630 with serum bicarbonate levels had an AUC of 0.79 (95%CI; 0.69-0.89, p<0.001) in identifying the severity of the disease. This data provides the first evidence that the microtranscriptome profiles of patients with severe leptospirosis were different from the non-severe group. Serum miR155-5p and miR630 levels might be novel biomarkers for identifying severe leptospirosis. |
format |
Article in Journal/Newspaper |
author |
Umaporn Limothai Janejira Dinhuzen Titipon Payongsri Sasipha Tachaboon Pisit Tangkijvanich Natthaya Chuaypen Nattachai Srisawat |
author_facet |
Umaporn Limothai Janejira Dinhuzen Titipon Payongsri Sasipha Tachaboon Pisit Tangkijvanich Natthaya Chuaypen Nattachai Srisawat |
author_sort |
Umaporn Limothai |
title |
Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis. |
title_short |
Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis. |
title_full |
Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis. |
title_fullStr |
Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis. |
title_full_unstemmed |
Circulating microtranscriptome profiles reveal distinct expression of microRNAs in severe leptospirosis. |
title_sort |
circulating microtranscriptome profiles reveal distinct expression of micrornas in severe leptospirosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008809 https://doaj.org/article/4f0faa2918564daeb3c0323b26652704 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008809 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008809 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008809 https://doaj.org/article/4f0faa2918564daeb3c0323b26652704 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008809 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
11 |
container_start_page |
e0008809 |
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1766340941857161216 |