A hamster-derived West Nile virus isolate induces persistent renal infection in mice.
West Nile virus (WNV) can persist long term in the brain and kidney tissues of humans, non-human primates, and hamsters. In this study, mice were infected with WNV strain H8912, previously cultured from the urine of a persistently infected hamster, to determine its pathogenesis in a murine host.We f...
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ftdoajarticles:oai:doaj.org/article:4f087291af7345b7a35f052214df6ed7 2023-05-15T15:12:38+02:00 A hamster-derived West Nile virus isolate induces persistent renal infection in mice. Vandana Saxena Guorui Xie Bei Li Tierra Farris Thomas Welte Bin Gong Paul Boor Ping Wu Shao-Jun Tang Robert Tesh Tian Wang 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002275 https://doaj.org/article/4f087291af7345b7a35f052214df6ed7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3681636?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002275 https://doaj.org/article/4f087291af7345b7a35f052214df6ed7 PLoS Neglected Tropical Diseases, Vol 7, Iss 6, p e2275 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002275 2022-12-31T09:49:45Z West Nile virus (WNV) can persist long term in the brain and kidney tissues of humans, non-human primates, and hamsters. In this study, mice were infected with WNV strain H8912, previously cultured from the urine of a persistently infected hamster, to determine its pathogenesis in a murine host.We found that WNV H8912 was highly attenuated for neuroinvasiveness in mice. Following a systemic infection, viral RNA could be detected quickly in blood and spleen and much later in kidneys. WNV H8912 induced constitutive IL-10 production, upregulation of IFN-β and IL-1β expression, and a specific IgM response on day 10 post-infection. WNV H8912 persisted preferentially in kidneys with mild renal inflammation, and less frequently in spleen for up to 2.5 months post infection. This was concurrent with detectable serum WNV-specific IgM and IgG production. There were also significantly fewer WNV- specific T cells and lower inflammatory responses in kidneys than in spleen. Previous studies have shown that systemic wild-type WNV NY99 infection induced virus persistence preferentially in spleen than in mouse kidneys. Here, we noted that splenocytes of WNV H8912-infected mice produced significantly less IL-10 than those of WNV NY99-infected mice. Finally, WNV H8912 was also attenuated in neurovirulence. Following intracranial inoculation, WNV persisted in the brain at a low frequency, concurrent with neither inflammatory responses nor neuronal damage in the brain.WNV H8912 is highly attenuated in both neuroinvasiveness and neurovirulence in mice. It induces a low and delayed anti-viral response in mice and preferentially persists in the kidneys. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 6 e2275 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vandana Saxena Guorui Xie Bei Li Tierra Farris Thomas Welte Bin Gong Paul Boor Ping Wu Shao-Jun Tang Robert Tesh Tian Wang A hamster-derived West Nile virus isolate induces persistent renal infection in mice. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
West Nile virus (WNV) can persist long term in the brain and kidney tissues of humans, non-human primates, and hamsters. In this study, mice were infected with WNV strain H8912, previously cultured from the urine of a persistently infected hamster, to determine its pathogenesis in a murine host.We found that WNV H8912 was highly attenuated for neuroinvasiveness in mice. Following a systemic infection, viral RNA could be detected quickly in blood and spleen and much later in kidneys. WNV H8912 induced constitutive IL-10 production, upregulation of IFN-β and IL-1β expression, and a specific IgM response on day 10 post-infection. WNV H8912 persisted preferentially in kidneys with mild renal inflammation, and less frequently in spleen for up to 2.5 months post infection. This was concurrent with detectable serum WNV-specific IgM and IgG production. There were also significantly fewer WNV- specific T cells and lower inflammatory responses in kidneys than in spleen. Previous studies have shown that systemic wild-type WNV NY99 infection induced virus persistence preferentially in spleen than in mouse kidneys. Here, we noted that splenocytes of WNV H8912-infected mice produced significantly less IL-10 than those of WNV NY99-infected mice. Finally, WNV H8912 was also attenuated in neurovirulence. Following intracranial inoculation, WNV persisted in the brain at a low frequency, concurrent with neither inflammatory responses nor neuronal damage in the brain.WNV H8912 is highly attenuated in both neuroinvasiveness and neurovirulence in mice. It induces a low and delayed anti-viral response in mice and preferentially persists in the kidneys. |
format |
Article in Journal/Newspaper |
author |
Vandana Saxena Guorui Xie Bei Li Tierra Farris Thomas Welte Bin Gong Paul Boor Ping Wu Shao-Jun Tang Robert Tesh Tian Wang |
author_facet |
Vandana Saxena Guorui Xie Bei Li Tierra Farris Thomas Welte Bin Gong Paul Boor Ping Wu Shao-Jun Tang Robert Tesh Tian Wang |
author_sort |
Vandana Saxena |
title |
A hamster-derived West Nile virus isolate induces persistent renal infection in mice. |
title_short |
A hamster-derived West Nile virus isolate induces persistent renal infection in mice. |
title_full |
A hamster-derived West Nile virus isolate induces persistent renal infection in mice. |
title_fullStr |
A hamster-derived West Nile virus isolate induces persistent renal infection in mice. |
title_full_unstemmed |
A hamster-derived West Nile virus isolate induces persistent renal infection in mice. |
title_sort |
hamster-derived west nile virus isolate induces persistent renal infection in mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002275 https://doaj.org/article/4f087291af7345b7a35f052214df6ed7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 6, p e2275 (2013) |
op_relation |
http://europepmc.org/articles/PMC3681636?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002275 https://doaj.org/article/4f087291af7345b7a35f052214df6ed7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002275 |
container_title |
PLoS Neglected Tropical Diseases |
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6 |
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e2275 |
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