Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem
E. coli was isolated from the Salish Sea (Puget Sound) ecosystem, including samples of marine and fresh water, and wildlife dependent on this environment. E. coli isolates were assessed for phenotypic and genotypic resistance to antibiotics. A total of 305 E. coli isolates was characterized from sam...
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ftdoajarticles:oai:doaj.org/article:4eecdf8f050c43388aaa9e98b0923aeb 2023-05-15T17:58:56+02:00 Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem Alexandria Vingino Marilyn C. Roberts Michelle Wainstein James West Stephanie A. Norman Dyanna Lambourn Jeffery Lahti Ryan Ruiz Marisa D’Angeli Scott J. Weissman Peter Rabinowitz 2021-10-01T00:00:00Z https://doi.org/10.3390/antibiotics10101201 https://doaj.org/article/4eecdf8f050c43388aaa9e98b0923aeb EN eng MDPI AG https://www.mdpi.com/2079-6382/10/10/1201 https://doaj.org/toc/2079-6382 doi:10.3390/antibiotics10101201 2079-6382 https://doaj.org/article/4eecdf8f050c43388aaa9e98b0923aeb Antibiotics, Vol 10, Iss 1201, p 1201 (2021) antibiotic resistance E. coli marine mammals environment river otters Therapeutics. Pharmacology RM1-950 article 2021 ftdoajarticles https://doi.org/10.3390/antibiotics10101201 2022-12-31T11:49:38Z E. coli was isolated from the Salish Sea (Puget Sound) ecosystem, including samples of marine and fresh water, and wildlife dependent on this environment. E. coli isolates were assessed for phenotypic and genotypic resistance to antibiotics. A total of 305 E. coli isolates was characterized from samples collected from: marine water obtained in four quadrants of the Salish Sea; select locations near beaches; fresh water from streams near marine beaches; and fecal samples from harbor porpoises ( Phocoena phocoena ), harbor seals ( Phoca vitulina ), river otters ( Lontra canadensis ), and English sole ( Parophrys vetulus ). Isolates were evaluated using antimicrobial susceptibility typing, whole-genome sequencing, fumC , and multilocus sequence typing. Resistance and virulence genes were identified from sequence data. Of the 305 isolates from Salish Sea samples, 20 (6.6%) of the E. coli were intermediate, and 31 (10.2%) were resistant to ≥1 class of antibiotics, with 26.9% of nonsusceptible (resistant and intermediate resistant) E. coli isolates from marine mammals and 70% from river otters. The proportion of nonsusceptible isolates from animals was significantly higher than samples taken from marine water ( p < 0.0001). A total of 196 unique STs was identified including 37 extraintestinal pathogenic E. coli (ExPEC)-associated STs [ST10, ST38, ST58, ST69, ST73, ST117, ST131, and ST405]. The study suggests that animals may be potential sentinels for antibiotic-resistant and ExPEC E. coli in the Salish Sea ecosystem. Article in Journal/Newspaper Phoca vitulina Phocoena phocoena Lontra Directory of Open Access Journals: DOAJ Articles Antibiotics 10 10 1201 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antibiotic resistance E. coli marine mammals environment river otters Therapeutics. Pharmacology RM1-950 |
spellingShingle |
antibiotic resistance E. coli marine mammals environment river otters Therapeutics. Pharmacology RM1-950 Alexandria Vingino Marilyn C. Roberts Michelle Wainstein James West Stephanie A. Norman Dyanna Lambourn Jeffery Lahti Ryan Ruiz Marisa D’Angeli Scott J. Weissman Peter Rabinowitz Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem |
topic_facet |
antibiotic resistance E. coli marine mammals environment river otters Therapeutics. Pharmacology RM1-950 |
description |
E. coli was isolated from the Salish Sea (Puget Sound) ecosystem, including samples of marine and fresh water, and wildlife dependent on this environment. E. coli isolates were assessed for phenotypic and genotypic resistance to antibiotics. A total of 305 E. coli isolates was characterized from samples collected from: marine water obtained in four quadrants of the Salish Sea; select locations near beaches; fresh water from streams near marine beaches; and fecal samples from harbor porpoises ( Phocoena phocoena ), harbor seals ( Phoca vitulina ), river otters ( Lontra canadensis ), and English sole ( Parophrys vetulus ). Isolates were evaluated using antimicrobial susceptibility typing, whole-genome sequencing, fumC , and multilocus sequence typing. Resistance and virulence genes were identified from sequence data. Of the 305 isolates from Salish Sea samples, 20 (6.6%) of the E. coli were intermediate, and 31 (10.2%) were resistant to ≥1 class of antibiotics, with 26.9% of nonsusceptible (resistant and intermediate resistant) E. coli isolates from marine mammals and 70% from river otters. The proportion of nonsusceptible isolates from animals was significantly higher than samples taken from marine water ( p < 0.0001). A total of 196 unique STs was identified including 37 extraintestinal pathogenic E. coli (ExPEC)-associated STs [ST10, ST38, ST58, ST69, ST73, ST117, ST131, and ST405]. The study suggests that animals may be potential sentinels for antibiotic-resistant and ExPEC E. coli in the Salish Sea ecosystem. |
format |
Article in Journal/Newspaper |
author |
Alexandria Vingino Marilyn C. Roberts Michelle Wainstein James West Stephanie A. Norman Dyanna Lambourn Jeffery Lahti Ryan Ruiz Marisa D’Angeli Scott J. Weissman Peter Rabinowitz |
author_facet |
Alexandria Vingino Marilyn C. Roberts Michelle Wainstein James West Stephanie A. Norman Dyanna Lambourn Jeffery Lahti Ryan Ruiz Marisa D’Angeli Scott J. Weissman Peter Rabinowitz |
author_sort |
Alexandria Vingino |
title |
Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem |
title_short |
Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem |
title_full |
Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem |
title_fullStr |
Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem |
title_full_unstemmed |
Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem |
title_sort |
surveillance for antibiotic-resistant e. coli in the salish sea ecosystem |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/antibiotics10101201 https://doaj.org/article/4eecdf8f050c43388aaa9e98b0923aeb |
genre |
Phoca vitulina Phocoena phocoena Lontra |
genre_facet |
Phoca vitulina Phocoena phocoena Lontra |
op_source |
Antibiotics, Vol 10, Iss 1201, p 1201 (2021) |
op_relation |
https://www.mdpi.com/2079-6382/10/10/1201 https://doaj.org/toc/2079-6382 doi:10.3390/antibiotics10101201 2079-6382 https://doaj.org/article/4eecdf8f050c43388aaa9e98b0923aeb |
op_doi |
https://doi.org/10.3390/antibiotics10101201 |
container_title |
Antibiotics |
container_volume |
10 |
container_issue |
10 |
container_start_page |
1201 |
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1766167666512363520 |