Post-diapause transcriptomic restarts: insight from a high-latitude copepod
Abstract Background Diapause is a seasonal dormancy that allows organisms to survive unfavorable conditions and optimizes the timing of reproduction and growth. Emergence from diapause reverses the state of arrested development and metabolic suppression returning the organism to an active state. The...
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ftdoajarticles:oai:doaj.org/article:4ee914aea10f453ea6020292c782ebc6 2023-05-15T15:15:46+02:00 Post-diapause transcriptomic restarts: insight from a high-latitude copepod Vittoria Roncalli Matthew C. Cieslak Ann M. Castelfranco Russell R. Hopcroft Daniel K. Hartline Petra H. Lenz 2021-06-01T00:00:00Z https://doi.org/10.1186/s12864-021-07557-7 https://doaj.org/article/4ee914aea10f453ea6020292c782ebc6 EN eng BMC https://doi.org/10.1186/s12864-021-07557-7 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-021-07557-7 1471-2164 https://doaj.org/article/4ee914aea10f453ea6020292c782ebc6 BMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021) Copepod Gulf of Alaska Neocalanus flemingeri Transcriptomics Diapause Respiration Biotechnology TP248.13-248.65 Genetics QH426-470 article 2021 ftdoajarticles https://doi.org/10.1186/s12864-021-07557-7 2022-12-31T11:36:20Z Abstract Background Diapause is a seasonal dormancy that allows organisms to survive unfavorable conditions and optimizes the timing of reproduction and growth. Emergence from diapause reverses the state of arrested development and metabolic suppression returning the organism to an active state. The physiological mechanisms that regulate the transition from diapause to post-diapause are still unknown. In this study, this transition has been characterized for the sub-arctic calanoid copepod Neocalanus flemingeri, a key crustacean zooplankter that supports the highly productive North Pacific fisheries. Transcriptional profiling of females, determined over a two-week time series starting with diapausing females collected from > 400 m depth, characterized the molecular mechanisms that regulate the post-diapause trajectory. Results A complex set of transitions in relative gene expression defined the transcriptomic changes from diapause to post-diapause. Despite low temperatures (5–6 °C), the switch from a “diapause” to a “post-diapause” transcriptional profile occurred within 12 h of the termination stimulus. Transcriptional changes signaling the end of diapause were activated within one-hour post collection and included the up-regulation of genes involved in the 20E cascade pathway, the TCA cycle and RNA metabolism in combination with the down-regulation of genes associated with chromatin silencing. By 12 h, females exhibited a post-diapause phenotype characterized by the up-regulation of genes involved in cell division, cell differentiation and multiple developmental processes. By seven days post collection, the reproductive program was fully activated as indicated by up-regulation of genes involved in oogenesis and energy metabolism, processes that were enriched among the differentially expressed genes. Conclusions The analysis revealed a finely structured, precisely orchestrated sequence of transcriptional changes that led to rapid changes in the activation of biological processes paving the way to the ... Article in Journal/Newspaper Arctic Alaska Directory of Open Access Journals: DOAJ Articles Arctic Gulf of Alaska Pacific BMC Genomics 22 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Copepod Gulf of Alaska Neocalanus flemingeri Transcriptomics Diapause Respiration Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Copepod Gulf of Alaska Neocalanus flemingeri Transcriptomics Diapause Respiration Biotechnology TP248.13-248.65 Genetics QH426-470 Vittoria Roncalli Matthew C. Cieslak Ann M. Castelfranco Russell R. Hopcroft Daniel K. Hartline Petra H. Lenz Post-diapause transcriptomic restarts: insight from a high-latitude copepod |
topic_facet |
Copepod Gulf of Alaska Neocalanus flemingeri Transcriptomics Diapause Respiration Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background Diapause is a seasonal dormancy that allows organisms to survive unfavorable conditions and optimizes the timing of reproduction and growth. Emergence from diapause reverses the state of arrested development and metabolic suppression returning the organism to an active state. The physiological mechanisms that regulate the transition from diapause to post-diapause are still unknown. In this study, this transition has been characterized for the sub-arctic calanoid copepod Neocalanus flemingeri, a key crustacean zooplankter that supports the highly productive North Pacific fisheries. Transcriptional profiling of females, determined over a two-week time series starting with diapausing females collected from > 400 m depth, characterized the molecular mechanisms that regulate the post-diapause trajectory. Results A complex set of transitions in relative gene expression defined the transcriptomic changes from diapause to post-diapause. Despite low temperatures (5–6 °C), the switch from a “diapause” to a “post-diapause” transcriptional profile occurred within 12 h of the termination stimulus. Transcriptional changes signaling the end of diapause were activated within one-hour post collection and included the up-regulation of genes involved in the 20E cascade pathway, the TCA cycle and RNA metabolism in combination with the down-regulation of genes associated with chromatin silencing. By 12 h, females exhibited a post-diapause phenotype characterized by the up-regulation of genes involved in cell division, cell differentiation and multiple developmental processes. By seven days post collection, the reproductive program was fully activated as indicated by up-regulation of genes involved in oogenesis and energy metabolism, processes that were enriched among the differentially expressed genes. Conclusions The analysis revealed a finely structured, precisely orchestrated sequence of transcriptional changes that led to rapid changes in the activation of biological processes paving the way to the ... |
format |
Article in Journal/Newspaper |
author |
Vittoria Roncalli Matthew C. Cieslak Ann M. Castelfranco Russell R. Hopcroft Daniel K. Hartline Petra H. Lenz |
author_facet |
Vittoria Roncalli Matthew C. Cieslak Ann M. Castelfranco Russell R. Hopcroft Daniel K. Hartline Petra H. Lenz |
author_sort |
Vittoria Roncalli |
title |
Post-diapause transcriptomic restarts: insight from a high-latitude copepod |
title_short |
Post-diapause transcriptomic restarts: insight from a high-latitude copepod |
title_full |
Post-diapause transcriptomic restarts: insight from a high-latitude copepod |
title_fullStr |
Post-diapause transcriptomic restarts: insight from a high-latitude copepod |
title_full_unstemmed |
Post-diapause transcriptomic restarts: insight from a high-latitude copepod |
title_sort |
post-diapause transcriptomic restarts: insight from a high-latitude copepod |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12864-021-07557-7 https://doaj.org/article/4ee914aea10f453ea6020292c782ebc6 |
geographic |
Arctic Gulf of Alaska Pacific |
geographic_facet |
Arctic Gulf of Alaska Pacific |
genre |
Arctic Alaska |
genre_facet |
Arctic Alaska |
op_source |
BMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021) |
op_relation |
https://doi.org/10.1186/s12864-021-07557-7 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-021-07557-7 1471-2164 https://doaj.org/article/4ee914aea10f453ea6020292c782ebc6 |
op_doi |
https://doi.org/10.1186/s12864-021-07557-7 |
container_title |
BMC Genomics |
container_volume |
22 |
container_issue |
1 |
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1766346117811798016 |