Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005
INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health inf...
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2011
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ftdoajarticles:oai:doaj.org/article:4ec9c4d64a9a4910a159d50b94ee681b 2023-05-15T15:11:28+02:00 Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz 2011-06-01T00:00:00Z https://doi.org/10.1590/s0037-86822011005000040 https://doaj.org/article/4ec9c4d64a9a4910a159d50b94ee681b EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300016&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000040 https://doaj.org/article/4ec9c4d64a9a4910a159d50b94ee681b Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 339-343 (2011) Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/s0037-86822011005000040 2022-12-31T03:06:27Z INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health information. Interviews and blood sample collections by finger prick on Whatman filter paper were performed from August 2004 to May 2005. Observation determined that 850 samples were suitable for analysis and were tested by ELISA using vesicular fluid of Taenia crassiceps heterologous antigen. To ensure the reliability of the results, 77 samples of the dried blood were matched with sera. The reactive samples were submitted to a serum confirmatory immunoblot (IB) test using purified Taenia crassiceps glycoproteins. RESULTS: The ELISA results for the dried blood and serum samples were statistically consistent. ELISA was positive in 186 (21.9%) out of 850 individuals. A group of 213 individuals were asked to collect vein blood for IB (186 with positive result in ELISA and 27 with inappropriate whole blood samples) and 130 attended the request. The IB was positive in 29 (3.4%) out of 850 individuals. A significant correlation (p = 0.0364) was determined among individuals who tested positive in the IB assay who practiced both pig rearing and kitchen gardening. CONCLUSIONS: ELISA with dried blood eluted from filter paper was suitable for cysticercosis population surveys. In Lages, human infection was associated with pig rearing and kitchen gardening. The prevalence index was compatible with other Latin American endemic areas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 3 339 343 |
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Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Arctic medicine. Tropical medicine RC955-962 |
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Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Arctic medicine. Tropical medicine RC955-962 Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
topic_facet |
Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health information. Interviews and blood sample collections by finger prick on Whatman filter paper were performed from August 2004 to May 2005. Observation determined that 850 samples were suitable for analysis and were tested by ELISA using vesicular fluid of Taenia crassiceps heterologous antigen. To ensure the reliability of the results, 77 samples of the dried blood were matched with sera. The reactive samples were submitted to a serum confirmatory immunoblot (IB) test using purified Taenia crassiceps glycoproteins. RESULTS: The ELISA results for the dried blood and serum samples were statistically consistent. ELISA was positive in 186 (21.9%) out of 850 individuals. A group of 213 individuals were asked to collect vein blood for IB (186 with positive result in ELISA and 27 with inappropriate whole blood samples) and 130 attended the request. The IB was positive in 29 (3.4%) out of 850 individuals. A significant correlation (p = 0.0364) was determined among individuals who tested positive in the IB assay who practiced both pig rearing and kitchen gardening. CONCLUSIONS: ELISA with dried blood eluted from filter paper was suitable for cysticercosis population surveys. In Lages, human infection was associated with pig rearing and kitchen gardening. The prevalence index was compatible with other Latin American endemic areas. |
format |
Article in Journal/Newspaper |
author |
Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz |
author_facet |
Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz |
author_sort |
Maria Márcia Imenes Ishida |
title |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
title_short |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
title_full |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
title_fullStr |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
title_full_unstemmed |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
title_sort |
seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in lages, state of santa catarina, brazil, 2004-2005 |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/s0037-86822011005000040 https://doaj.org/article/4ec9c4d64a9a4910a159d50b94ee681b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 339-343 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300016&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000040 https://doaj.org/article/4ec9c4d64a9a4910a159d50b94ee681b |
op_doi |
https://doi.org/10.1590/s0037-86822011005000040 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
44 |
container_issue |
3 |
container_start_page |
339 |
op_container_end_page |
343 |
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1766342312773812224 |