Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment.
Epidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated,...
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ftdoajarticles:oai:doaj.org/article:4e97c666c9cc457eb48530f44b0ac7a6 2023-05-15T15:11:31+02:00 Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. Leopold D Tientcheu Mariëlle C Haks Schadrac C Agbla Jayne S Sutherland Ifedayo M Adetifa Simon Donkor Edwin Quinten Mohammed Daramy Martin Antonio Beate Kampmann Tom H M Ottenhoff Hazel M Dockrell Martin O Ota 2016-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004701 https://doaj.org/article/4e97c666c9cc457eb48530f44b0ac7a6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4871581?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004701 https://doaj.org/article/4e97c666c9cc457eb48530f44b0ac7a6 PLoS Neglected Tropical Diseases, Vol 10, Iss 5, p e0004701 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004701 2022-12-31T08:30:45Z Epidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated, and ESAT6/CFP-10-, whole-Maf- and Mtb-stimulated blood samples of 26 Maf- and 49 Mtb-HIV-negative tuberculosis patients before, and after 2 and 6 months of anti-tuberculosis therapy. Before treatment, both groups had similar clinical parameters, but differed in few cytokines concentration and gene expression profiles. Following treatment the body mass index, skinfold thickness and chest X-ray scores showed greater improvement in the Mtb- compared to Maf-infected patients, after adjusting for age, sex and ethnicity (p = 0.02; 0.04 and 0.007, respectively). In addition, in unstimulated blood, IL-12p70, IL12A and TLR9 were significantly higher in Maf-infected patients, while IL-15, IL-8 and MIP-1α were higher in Mtb-infected patients. Overnight stimulation with ESAT-6/CFP-10 induced significantly higher levels of IFN-γ and TNF-α production, as well as gene expression of CCL4, IL1B and TLR4 in Mtb- compared to Maf-infected patients. Our study confirms differences in clinical features and immune genes expression and concentration of proteins associated with inflammatory processes between Mtb- and Maf-infected patients following anti-tuberculosis treatment These findings have public health implications for treatment regimens, and biomarkers for tuberculosis diagnosis and susceptibility. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 5 e0004701 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Leopold D Tientcheu Mariëlle C Haks Schadrac C Agbla Jayne S Sutherland Ifedayo M Adetifa Simon Donkor Edwin Quinten Mohammed Daramy Martin Antonio Beate Kampmann Tom H M Ottenhoff Hazel M Dockrell Martin O Ota Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Epidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated, and ESAT6/CFP-10-, whole-Maf- and Mtb-stimulated blood samples of 26 Maf- and 49 Mtb-HIV-negative tuberculosis patients before, and after 2 and 6 months of anti-tuberculosis therapy. Before treatment, both groups had similar clinical parameters, but differed in few cytokines concentration and gene expression profiles. Following treatment the body mass index, skinfold thickness and chest X-ray scores showed greater improvement in the Mtb- compared to Maf-infected patients, after adjusting for age, sex and ethnicity (p = 0.02; 0.04 and 0.007, respectively). In addition, in unstimulated blood, IL-12p70, IL12A and TLR9 were significantly higher in Maf-infected patients, while IL-15, IL-8 and MIP-1α were higher in Mtb-infected patients. Overnight stimulation with ESAT-6/CFP-10 induced significantly higher levels of IFN-γ and TNF-α production, as well as gene expression of CCL4, IL1B and TLR4 in Mtb- compared to Maf-infected patients. Our study confirms differences in clinical features and immune genes expression and concentration of proteins associated with inflammatory processes between Mtb- and Maf-infected patients following anti-tuberculosis treatment These findings have public health implications for treatment regimens, and biomarkers for tuberculosis diagnosis and susceptibility. |
format |
Article in Journal/Newspaper |
author |
Leopold D Tientcheu Mariëlle C Haks Schadrac C Agbla Jayne S Sutherland Ifedayo M Adetifa Simon Donkor Edwin Quinten Mohammed Daramy Martin Antonio Beate Kampmann Tom H M Ottenhoff Hazel M Dockrell Martin O Ota |
author_facet |
Leopold D Tientcheu Mariëlle C Haks Schadrac C Agbla Jayne S Sutherland Ifedayo M Adetifa Simon Donkor Edwin Quinten Mohammed Daramy Martin Antonio Beate Kampmann Tom H M Ottenhoff Hazel M Dockrell Martin O Ota |
author_sort |
Leopold D Tientcheu |
title |
Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
title_short |
Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
title_full |
Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
title_fullStr |
Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
title_full_unstemmed |
Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment. |
title_sort |
host immune responses differ between m. africanum- and m. tuberculosis-infected patients following standard anti-tuberculosis treatment. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004701 https://doaj.org/article/4e97c666c9cc457eb48530f44b0ac7a6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 5, p e0004701 (2016) |
op_relation |
http://europepmc.org/articles/PMC4871581?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004701 https://doaj.org/article/4e97c666c9cc457eb48530f44b0ac7a6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004701 |
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PLOS Neglected Tropical Diseases |
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10 |
container_issue |
5 |
container_start_page |
e0004701 |
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