Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission
Abstract Background A vaccine that targets multiple developmental stages of malaria parasites would be an effective tool for malaria control and elimination. Methods A conserved gene in Plasmodium, the Plasmodium berghei gene (PBANKA_020570) encoding a 51 kDa protein (pb51 gene), was identified thro...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-017-2107-2 https://doaj.org/article/4e87b00e81024741b50fbf17ede34dc3 |
id |
ftdoajarticles:oai:doaj.org/article:4e87b00e81024741b50fbf17ede34dc3 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:4e87b00e81024741b50fbf17ede34dc3 2023-05-15T15:15:54+02:00 Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission Jian Wang Wenqi Zheng Fei Liu Yaru Wang Yiwen He Li Zheng Qi Fan Enjie Luo Yaming Cao Liwang Cui 2017-11-01T00:00:00Z https://doi.org/10.1186/s12936-017-2107-2 https://doaj.org/article/4e87b00e81024741b50fbf17ede34dc3 EN eng BMC http://link.springer.com/article/10.1186/s12936-017-2107-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2107-2 1475-2875 https://doaj.org/article/4e87b00e81024741b50fbf17ede34dc3 Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) Plasmodium berghei Pb51 Vaccine candidate Asexual blood stage Sexual stage Transmission-blocking Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-2107-2 2022-12-31T14:16:39Z Abstract Background A vaccine that targets multiple developmental stages of malaria parasites would be an effective tool for malaria control and elimination. Methods A conserved gene in Plasmodium, the Plasmodium berghei gene (PBANKA_020570) encoding a 51 kDa protein (pb51 gene), was identified through search of the PlasmoDB database using a combination of expression and protein localization criteria. A partial domain of the Pb51 protein was expressed in a prokaryotic expression system (rPb51) and used for immunization in mice. The protein expression profile and localization were studied by Western blot and indirect immunofluorescence assay (IFA), respectively. The inhibitory effect of the anti-rPb51 antibodies on parasite proliferation was evaluated in erythrocytes in vivo. The transmission-blocking activity of the immune sera was determined by in vitro ookinete conversion assay and by direct mosquito feeding assay (DFA). Results The rPb51 elicited specific antibodies in mice. Western blot confirmed Pb51 expression in schizonts, gametocytes and ookinetes. IFA showed localization of Pb51 on the outer membranes of schizonts, gametocytes, zygotes, retorts, ookinetes and sporozoites of P. berghei. Mice immunized with the rPb51 protein significantly reduced parasite proliferation and gametocyte conversion in vivo. Moreover, the rPb51 antisera also significantly reduced the in vitro ookinete conversion when added into the ookinete culture medium. In DFA, mice immunized with the rPb51 reduced the prevalence of mosquito infection by 21.3% and oocyst density by 54.8%. Conclusions In P. berghei, P51 was expressed in both asexual erythrocytic and sexual stages and localized on the surface of these stages with the exception of the ring stage. The anti-rPb51 antibodies inhibited both P. berghei proliferation in mice and transmission of the parasite to mosquitoes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium berghei Pb51 Vaccine candidate Asexual blood stage Sexual stage Transmission-blocking Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium berghei Pb51 Vaccine candidate Asexual blood stage Sexual stage Transmission-blocking Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jian Wang Wenqi Zheng Fei Liu Yaru Wang Yiwen He Li Zheng Qi Fan Enjie Luo Yaming Cao Liwang Cui Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission |
topic_facet |
Plasmodium berghei Pb51 Vaccine candidate Asexual blood stage Sexual stage Transmission-blocking Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background A vaccine that targets multiple developmental stages of malaria parasites would be an effective tool for malaria control and elimination. Methods A conserved gene in Plasmodium, the Plasmodium berghei gene (PBANKA_020570) encoding a 51 kDa protein (pb51 gene), was identified through search of the PlasmoDB database using a combination of expression and protein localization criteria. A partial domain of the Pb51 protein was expressed in a prokaryotic expression system (rPb51) and used for immunization in mice. The protein expression profile and localization were studied by Western blot and indirect immunofluorescence assay (IFA), respectively. The inhibitory effect of the anti-rPb51 antibodies on parasite proliferation was evaluated in erythrocytes in vivo. The transmission-blocking activity of the immune sera was determined by in vitro ookinete conversion assay and by direct mosquito feeding assay (DFA). Results The rPb51 elicited specific antibodies in mice. Western blot confirmed Pb51 expression in schizonts, gametocytes and ookinetes. IFA showed localization of Pb51 on the outer membranes of schizonts, gametocytes, zygotes, retorts, ookinetes and sporozoites of P. berghei. Mice immunized with the rPb51 protein significantly reduced parasite proliferation and gametocyte conversion in vivo. Moreover, the rPb51 antisera also significantly reduced the in vitro ookinete conversion when added into the ookinete culture medium. In DFA, mice immunized with the rPb51 reduced the prevalence of mosquito infection by 21.3% and oocyst density by 54.8%. Conclusions In P. berghei, P51 was expressed in both asexual erythrocytic and sexual stages and localized on the surface of these stages with the exception of the ring stage. The anti-rPb51 antibodies inhibited both P. berghei proliferation in mice and transmission of the parasite to mosquitoes. |
format |
Article in Journal/Newspaper |
author |
Jian Wang Wenqi Zheng Fei Liu Yaru Wang Yiwen He Li Zheng Qi Fan Enjie Luo Yaming Cao Liwang Cui |
author_facet |
Jian Wang Wenqi Zheng Fei Liu Yaru Wang Yiwen He Li Zheng Qi Fan Enjie Luo Yaming Cao Liwang Cui |
author_sort |
Jian Wang |
title |
Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission |
title_short |
Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission |
title_full |
Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission |
title_fullStr |
Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission |
title_full_unstemmed |
Characterization of Pb51 in Plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission |
title_sort |
characterization of pb51 in plasmodium berghei as a malaria vaccine candidate targeting both asexual erythrocytic proliferation and transmission |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-2107-2 https://doaj.org/article/4e87b00e81024741b50fbf17ede34dc3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-2107-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2107-2 1475-2875 https://doaj.org/article/4e87b00e81024741b50fbf17ede34dc3 |
op_doi |
https://doi.org/10.1186/s12936-017-2107-2 |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
_version_ |
1766346224789618688 |