Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica.
Mitochondrion-related organelles, mitosomes and hydrogenosomes, are found in a phylogenetically broad range of organisms. Their components and functions are highly diverse. We have previously shown that mitosomes of the anaerobic/microaerophilic intestinal protozoan parasite Entamoeba histolytica ha...
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ftdoajarticles:oai:doaj.org/article:4e8663756d9745c3b4c8f2ff3f4d1b35 2023-05-15T15:06:34+02:00 Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica. Fumika Mi-ichi Takashi Makiuchi Atsushi Furukawa Dan Sato Tomoyoshi Nozaki 2011-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001263 https://doaj.org/article/4e8663756d9745c3b4c8f2ff3f4d1b35 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3149026?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0001263 1935-2727 1935-2735 https://doaj.org/article/4e8663756d9745c3b4c8f2ff3f4d1b35 PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1263 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001263 2022-12-31T06:01:40Z Mitochondrion-related organelles, mitosomes and hydrogenosomes, are found in a phylogenetically broad range of organisms. Their components and functions are highly diverse. We have previously shown that mitosomes of the anaerobic/microaerophilic intestinal protozoan parasite Entamoeba histolytica have uniquely evolved and compartmentalized a sulfate activation pathway. Although this confined metabolic pathway is the major function in E. histolytica mitosomes, their physiological role remains unknown. In this study, we examined the phenotypes of the parasites in which genes involved in the mitosome functions were suppressed by gene silencing, and showed that sulfate activation in mitosomes is important for sulfolipid synthesis and cell proliferation. We also demonstrated that both Cpn60 and unusual mitochondrial ADP/ATP transporter (mitochondria carrier family, MCF) are important for the mitosome functions. Immunoelectron microscopy demonstrated that the enzymes involved in sulfate activation, Cpn60, and mitochondrial carrier family were differentially distributed within the electron dense, double membrane-bounded organelles. The importance and topology of the components in E. histolytica mitosomes reinforce the notion that they are not "rudimentary" or "residual" mitochondria, but represent a uniquely evolved crucial organelle in E. histolytica. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 8 e1263 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Fumika Mi-ichi Takashi Makiuchi Atsushi Furukawa Dan Sato Tomoyoshi Nozaki Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mitochondrion-related organelles, mitosomes and hydrogenosomes, are found in a phylogenetically broad range of organisms. Their components and functions are highly diverse. We have previously shown that mitosomes of the anaerobic/microaerophilic intestinal protozoan parasite Entamoeba histolytica have uniquely evolved and compartmentalized a sulfate activation pathway. Although this confined metabolic pathway is the major function in E. histolytica mitosomes, their physiological role remains unknown. In this study, we examined the phenotypes of the parasites in which genes involved in the mitosome functions were suppressed by gene silencing, and showed that sulfate activation in mitosomes is important for sulfolipid synthesis and cell proliferation. We also demonstrated that both Cpn60 and unusual mitochondrial ADP/ATP transporter (mitochondria carrier family, MCF) are important for the mitosome functions. Immunoelectron microscopy demonstrated that the enzymes involved in sulfate activation, Cpn60, and mitochondrial carrier family were differentially distributed within the electron dense, double membrane-bounded organelles. The importance and topology of the components in E. histolytica mitosomes reinforce the notion that they are not "rudimentary" or "residual" mitochondria, but represent a uniquely evolved crucial organelle in E. histolytica. |
format |
Article in Journal/Newspaper |
author |
Fumika Mi-ichi Takashi Makiuchi Atsushi Furukawa Dan Sato Tomoyoshi Nozaki |
author_facet |
Fumika Mi-ichi Takashi Makiuchi Atsushi Furukawa Dan Sato Tomoyoshi Nozaki |
author_sort |
Fumika Mi-ichi |
title |
Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica. |
title_short |
Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica. |
title_full |
Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica. |
title_fullStr |
Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica. |
title_full_unstemmed |
Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica. |
title_sort |
sulfate activation in mitosomes plays an important role in the proliferation of entamoeba histolytica. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001263 https://doaj.org/article/4e8663756d9745c3b4c8f2ff3f4d1b35 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1263 (2011) |
op_relation |
http://europepmc.org/articles/PMC3149026?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0001263 1935-2727 1935-2735 https://doaj.org/article/4e8663756d9745c3b4c8f2ff3f4d1b35 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001263 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
8 |
container_start_page |
e1263 |
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1766338143611518976 |