A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae
The gal80 mutant of yeast Saccharomyces cerevisiae is used for the constitutive expression under strong GAL promoters without galactose induction. To enhance productivity of gal80 mutant, an alternative strain, allgal, was developed by removing all galactose-utilizing genes that consume significant...
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ftdoajarticles:oai:doaj.org/article:4e7a03336f3a449f90ddc4763b53d6c6 2023-05-15T13:31:08+02:00 A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae Mi-Jin Kim Bong Hyun Sung Hyun-Joo Park Jung-Hoon Sohn Jung-Hoon Bae 2022-12-01T00:00:00Z https://doi.org/10.1016/j.btre.2022.e00763 https://doaj.org/article/4e7a03336f3a449f90ddc4763b53d6c6 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2215017X22000613 https://doaj.org/toc/2215-017X 2215-017X doi:10.1016/j.btre.2022.e00763 https://doaj.org/article/4e7a03336f3a449f90ddc4763b53d6c6 Biotechnology Reports, Vol 36, Iss , Pp e00763- (2022) Galactose GAL10 promoter Recombinant proteins Constitutive expression Saccharomyces cerevisiae Biotechnology TP248.13-248.65 article 2022 ftdoajarticles https://doi.org/10.1016/j.btre.2022.e00763 2022-12-30T20:37:48Z The gal80 mutant of yeast Saccharomyces cerevisiae is used for the constitutive expression under strong GAL promoters without galactose induction. To enhance productivity of gal80 mutant, an alternative strain, allgal, was developed by removing all galactose-utilizing genes that consume significant cellular resources in the gal80 strain when cultured in non-galactose conditions. The efficacy of the allgal mutant (gal80, gal1, gal2, gal7, and gal10) was verified by assessing the secretory expression of three recombinant proteins, Candida antarctica lipase B (CalB), human serum albumin (HSA), and human epidermal growth factor (hEGF), using the GAL10 promoter. The growth of the allgal mutant was enhanced by 15–38% compared to the gal80 mutant, and the secretion of recombinant proteins also increased by 16–22% in fed-batch fermentation. Thus, the expression of recombinant proteins using GAL10 promoter in the allgal mutant is suitable for the economical production of recombinant proteins in S. cerevisiae. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Biotechnology Reports 36 e00763 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Galactose GAL10 promoter Recombinant proteins Constitutive expression Saccharomyces cerevisiae Biotechnology TP248.13-248.65 |
spellingShingle |
Galactose GAL10 promoter Recombinant proteins Constitutive expression Saccharomyces cerevisiae Biotechnology TP248.13-248.65 Mi-Jin Kim Bong Hyun Sung Hyun-Joo Park Jung-Hoon Sohn Jung-Hoon Bae A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae |
topic_facet |
Galactose GAL10 promoter Recombinant proteins Constitutive expression Saccharomyces cerevisiae Biotechnology TP248.13-248.65 |
description |
The gal80 mutant of yeast Saccharomyces cerevisiae is used for the constitutive expression under strong GAL promoters without galactose induction. To enhance productivity of gal80 mutant, an alternative strain, allgal, was developed by removing all galactose-utilizing genes that consume significant cellular resources in the gal80 strain when cultured in non-galactose conditions. The efficacy of the allgal mutant (gal80, gal1, gal2, gal7, and gal10) was verified by assessing the secretory expression of three recombinant proteins, Candida antarctica lipase B (CalB), human serum albumin (HSA), and human epidermal growth factor (hEGF), using the GAL10 promoter. The growth of the allgal mutant was enhanced by 15–38% compared to the gal80 mutant, and the secretion of recombinant proteins also increased by 16–22% in fed-batch fermentation. Thus, the expression of recombinant proteins using GAL10 promoter in the allgal mutant is suitable for the economical production of recombinant proteins in S. cerevisiae. |
format |
Article in Journal/Newspaper |
author |
Mi-Jin Kim Bong Hyun Sung Hyun-Joo Park Jung-Hoon Sohn Jung-Hoon Bae |
author_facet |
Mi-Jin Kim Bong Hyun Sung Hyun-Joo Park Jung-Hoon Sohn Jung-Hoon Bae |
author_sort |
Mi-Jin Kim |
title |
A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae |
title_short |
A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae |
title_full |
A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae |
title_fullStr |
A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae |
title_full_unstemmed |
A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae |
title_sort |
new platform host for strong expression under gal promoters without inducer in saccharomyces cerevisiae |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.btre.2022.e00763 https://doaj.org/article/4e7a03336f3a449f90ddc4763b53d6c6 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology Reports, Vol 36, Iss , Pp e00763- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2215017X22000613 https://doaj.org/toc/2215-017X 2215-017X doi:10.1016/j.btre.2022.e00763 https://doaj.org/article/4e7a03336f3a449f90ddc4763b53d6c6 |
op_doi |
https://doi.org/10.1016/j.btre.2022.e00763 |
container_title |
Biotechnology Reports |
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36 |
container_start_page |
e00763 |
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1766016338257510400 |