A microbial feed additive abates intestinal inflammation in Atlantic salmon
The efficacy of a microbial feed additive (Bactocell®) in countering intestinal inflammation in Atlantic salmon was examined in this study. Fish were fed either the additive-coated feed (probiotic) or feed without it (control). After an initial 3-week feeding, an inflammatory condition was induced b...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2015
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fimmu.2015.00409 https://doaj.org/article/4e21de892fa944c1808b924e4c01aae2 |
id |
ftdoajarticles:oai:doaj.org/article:4e21de892fa944c1808b924e4c01aae2 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:4e21de892fa944c1808b924e4c01aae2 2023-05-15T15:29:56+02:00 A microbial feed additive abates intestinal inflammation in Atlantic salmon Ghana eVasanth Viswanath eKiron Amod eKulkarni Dalia eDahle Jep eLokesh Yoichiro eKitani 2015-08-01T00:00:00Z https://doi.org/10.3389/fimmu.2015.00409 https://doaj.org/article/4e21de892fa944c1808b924e4c01aae2 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00409/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2015.00409 https://doaj.org/article/4e21de892fa944c1808b924e4c01aae2 Frontiers in Immunology, Vol 6 (2015) Genes Histology Proteins probiotic intestinal inflammation Atlantic salmon Immunologic diseases. Allergy RC581-607 article 2015 ftdoajarticles https://doi.org/10.3389/fimmu.2015.00409 2022-12-30T20:57:38Z The efficacy of a microbial feed additive (Bactocell®) in countering intestinal inflammation in Atlantic salmon was examined in this study. Fish were fed either the additive-coated feed (probiotic) or feed without it (control). After an initial 3-week feeding, an inflammatory condition was induced by anally intubating all the fish with oxazolone. The fish were offered the feeds for 3 more weeks. Distal intestine from the groups was obtained at 4, 24 h, and 3 weeks, after oxazolone treatment.Inflammatory responses were prominent in both groups at 24 h, documented by changes in intestinal micromorphology, expression of inflammation-related genes and intestinal proteome. The control group was characterized by oedema, widening of intestinal villi and lamina propria, infiltration of granulocytes and lymphocytes, and higher expression of genes related to inflammatory responses, mul1b, il1b, tnfa, ifng, compared to the probiotic group or other time points of the control group. Further, the protein expression in the probiotic group at 24 h after inducing inflammation revealed 5 differentially regulated proteins - Calr, Psma5, Trp1, Ctsb and Naga. At 3 weeks after intubation, the inflammatory responses subsided in the probiotic group. The findings provide evidence that the microbial additive contributes to intestinal homeostasis in Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 6 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Genes Histology Proteins probiotic intestinal inflammation Atlantic salmon Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Genes Histology Proteins probiotic intestinal inflammation Atlantic salmon Immunologic diseases. Allergy RC581-607 Ghana eVasanth Viswanath eKiron Amod eKulkarni Dalia eDahle Jep eLokesh Yoichiro eKitani A microbial feed additive abates intestinal inflammation in Atlantic salmon |
topic_facet |
Genes Histology Proteins probiotic intestinal inflammation Atlantic salmon Immunologic diseases. Allergy RC581-607 |
description |
The efficacy of a microbial feed additive (Bactocell®) in countering intestinal inflammation in Atlantic salmon was examined in this study. Fish were fed either the additive-coated feed (probiotic) or feed without it (control). After an initial 3-week feeding, an inflammatory condition was induced by anally intubating all the fish with oxazolone. The fish were offered the feeds for 3 more weeks. Distal intestine from the groups was obtained at 4, 24 h, and 3 weeks, after oxazolone treatment.Inflammatory responses were prominent in both groups at 24 h, documented by changes in intestinal micromorphology, expression of inflammation-related genes and intestinal proteome. The control group was characterized by oedema, widening of intestinal villi and lamina propria, infiltration of granulocytes and lymphocytes, and higher expression of genes related to inflammatory responses, mul1b, il1b, tnfa, ifng, compared to the probiotic group or other time points of the control group. Further, the protein expression in the probiotic group at 24 h after inducing inflammation revealed 5 differentially regulated proteins - Calr, Psma5, Trp1, Ctsb and Naga. At 3 weeks after intubation, the inflammatory responses subsided in the probiotic group. The findings provide evidence that the microbial additive contributes to intestinal homeostasis in Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Ghana eVasanth Viswanath eKiron Amod eKulkarni Dalia eDahle Jep eLokesh Yoichiro eKitani |
author_facet |
Ghana eVasanth Viswanath eKiron Amod eKulkarni Dalia eDahle Jep eLokesh Yoichiro eKitani |
author_sort |
Ghana eVasanth |
title |
A microbial feed additive abates intestinal inflammation in Atlantic salmon |
title_short |
A microbial feed additive abates intestinal inflammation in Atlantic salmon |
title_full |
A microbial feed additive abates intestinal inflammation in Atlantic salmon |
title_fullStr |
A microbial feed additive abates intestinal inflammation in Atlantic salmon |
title_full_unstemmed |
A microbial feed additive abates intestinal inflammation in Atlantic salmon |
title_sort |
microbial feed additive abates intestinal inflammation in atlantic salmon |
publisher |
Frontiers Media S.A. |
publishDate |
2015 |
url |
https://doi.org/10.3389/fimmu.2015.00409 https://doaj.org/article/4e21de892fa944c1808b924e4c01aae2 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Frontiers in Immunology, Vol 6 (2015) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00409/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2015.00409 https://doaj.org/article/4e21de892fa944c1808b924e4c01aae2 |
op_doi |
https://doi.org/10.3389/fimmu.2015.00409 |
container_title |
Frontiers in Immunology |
container_volume |
6 |
_version_ |
1766360387602612224 |