The microbiome in urogenital schistosomiasis and induced bladder pathologies.
BACKGROUND:Human schistosomiasis is a highly prevalent neglected tropical disease (NTD) caused by Schistosoma species. Research on the molecular mechanisms influencing the outcomes of bladder infection by Schistosoma haematobium is urgently needed to develop new diagnostics, therapeutics and infecti...
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ftdoajarticles:oai:doaj.org/article:4dda90b8ba3d4e8c92681935e740f197 2023-05-15T15:12:19+02:00 The microbiome in urogenital schistosomiasis and induced bladder pathologies. Adewale S Adebayo Mangesh Vasant Suryavanshi Shrikant Bhute Atinuke M Agunloye Raphael D Isokpehi Chiaka I Anumudu Yogesh S Shouche 2017-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005826 https://doaj.org/article/4dda90b8ba3d4e8c92681935e740f197 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5565189?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005826 https://doaj.org/article/4dda90b8ba3d4e8c92681935e740f197 PLoS Neglected Tropical Diseases, Vol 11, Iss 8, p e0005826 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005826 2022-12-31T10:38:05Z BACKGROUND:Human schistosomiasis is a highly prevalent neglected tropical disease (NTD) caused by Schistosoma species. Research on the molecular mechanisms influencing the outcomes of bladder infection by Schistosoma haematobium is urgently needed to develop new diagnostics, therapeutics and infection prevention strategies. The objective of the research study was to determine the microbiome features and changes in urine during urogenital schistosomiasis and induced bladder pathologies. METHODOLOGY:Seventy participants from Eggua, southwestern Nigeria provided morning urine samples and were screened for urogenital schistosomiasis infection and bladder pathologies in a cross-sectional study. Highthroughput NGS sequencing was carried out, targeting the 16S V3 region. Filtered reads were processed and analyzed in a bioinformatics pipeline. PRINCIPAL FINDINGS:The study participants (36 males and 34 females, between ages 15 and 65) were categorized into four groups according to status of schistosomiasis infection and bladder pathology. Data analytics of the next-generation sequencing reads revealed that Proteobacteria and Firmicutes dominated and had influence on microbiome structure of both non-infected persons and persons with urogenital schistosomiasis. Furthermore, gender and age influenced taxa abundance independent of infection or bladder pathology. Several taxa distinguished urogenital schistosomiasis induced bladder pathologies from urogenital schistosomiasis infection alone and from healthy persons, including known immune-stimulatory taxa such as Fusobacterium, Sphingobacterium and Enterococcus. Some of these significant taxa, especially Sphingobacterium were projected as markers of infection, while several genera including potentially beneficial taxa such as Trabulsiella and Weissella, were markers of the non-infected. Finally, expected changes in protein functional categories were observed to relate to cellular maintenance and lipid metabolism. CONCLUSION:The urinary microbiome is a factor to be considered ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 8 e0005826 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Adewale S Adebayo Mangesh Vasant Suryavanshi Shrikant Bhute Atinuke M Agunloye Raphael D Isokpehi Chiaka I Anumudu Yogesh S Shouche The microbiome in urogenital schistosomiasis and induced bladder pathologies. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Human schistosomiasis is a highly prevalent neglected tropical disease (NTD) caused by Schistosoma species. Research on the molecular mechanisms influencing the outcomes of bladder infection by Schistosoma haematobium is urgently needed to develop new diagnostics, therapeutics and infection prevention strategies. The objective of the research study was to determine the microbiome features and changes in urine during urogenital schistosomiasis and induced bladder pathologies. METHODOLOGY:Seventy participants from Eggua, southwestern Nigeria provided morning urine samples and were screened for urogenital schistosomiasis infection and bladder pathologies in a cross-sectional study. Highthroughput NGS sequencing was carried out, targeting the 16S V3 region. Filtered reads were processed and analyzed in a bioinformatics pipeline. PRINCIPAL FINDINGS:The study participants (36 males and 34 females, between ages 15 and 65) were categorized into four groups according to status of schistosomiasis infection and bladder pathology. Data analytics of the next-generation sequencing reads revealed that Proteobacteria and Firmicutes dominated and had influence on microbiome structure of both non-infected persons and persons with urogenital schistosomiasis. Furthermore, gender and age influenced taxa abundance independent of infection or bladder pathology. Several taxa distinguished urogenital schistosomiasis induced bladder pathologies from urogenital schistosomiasis infection alone and from healthy persons, including known immune-stimulatory taxa such as Fusobacterium, Sphingobacterium and Enterococcus. Some of these significant taxa, especially Sphingobacterium were projected as markers of infection, while several genera including potentially beneficial taxa such as Trabulsiella and Weissella, were markers of the non-infected. Finally, expected changes in protein functional categories were observed to relate to cellular maintenance and lipid metabolism. CONCLUSION:The urinary microbiome is a factor to be considered ... |
format |
Article in Journal/Newspaper |
author |
Adewale S Adebayo Mangesh Vasant Suryavanshi Shrikant Bhute Atinuke M Agunloye Raphael D Isokpehi Chiaka I Anumudu Yogesh S Shouche |
author_facet |
Adewale S Adebayo Mangesh Vasant Suryavanshi Shrikant Bhute Atinuke M Agunloye Raphael D Isokpehi Chiaka I Anumudu Yogesh S Shouche |
author_sort |
Adewale S Adebayo |
title |
The microbiome in urogenital schistosomiasis and induced bladder pathologies. |
title_short |
The microbiome in urogenital schistosomiasis and induced bladder pathologies. |
title_full |
The microbiome in urogenital schistosomiasis and induced bladder pathologies. |
title_fullStr |
The microbiome in urogenital schistosomiasis and induced bladder pathologies. |
title_full_unstemmed |
The microbiome in urogenital schistosomiasis and induced bladder pathologies. |
title_sort |
microbiome in urogenital schistosomiasis and induced bladder pathologies. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005826 https://doaj.org/article/4dda90b8ba3d4e8c92681935e740f197 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 8, p e0005826 (2017) |
op_relation |
http://europepmc.org/articles/PMC5565189?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005826 https://doaj.org/article/4dda90b8ba3d4e8c92681935e740f197 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005826 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
8 |
container_start_page |
e0005826 |
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1766343012128915456 |