Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt.
Myiasis is the infestation of human tissues by dipterous fly larvae of the class Insecta. Clogmia albipunctatus, family Psychodidae, is one of the most medically important insects that cause human myiasis. The aim of the present study is the morphological identification and the molecular characteriz...
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ftdoajarticles:oai:doaj.org/article:4dd15cee52c64435844f64322b3dabbe 2023-05-15T15:03:59+02:00 Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt. Haiam Mohammed Mahmoud Farrag Enas Abdelhameed Mahmoud Huseein Amal M Almatary Ragaa A Othman 2019-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007887 https://doaj.org/article/4dd15cee52c64435844f64322b3dabbe EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007887 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007887 https://doaj.org/article/4dd15cee52c64435844f64322b3dabbe PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007887 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007887 2022-12-31T13:13:07Z Myiasis is the infestation of human tissues by dipterous fly larvae of the class Insecta. Clogmia albipunctatus, family Psychodidae, is one of the most medically important insects that cause human myiasis. The aim of the present study is the morphological identification and the molecular characterization of moth flies causing many cases of urinary myiasis in Egypt, based on sequencing of the mitochondrial DNA of the larvae. Seven urinary samples of patients complaining of urinary symptoms and giving a history of low socioeconomic level were examined. Recovered larvae were identified using light microscopy and SEM. For molecular identification, the mitochondrial genes Cytochrome B (cytB), NADH1, NADH1, and 16S were sequenced and phylogenetically analyzed. The morphological and molecular characterization could accurately diagnose our patients to have C. albipunctatus infestation. Such results provided the initial set of data on the molecular identification and phylogenetic analysis of moth flies based on DNA barcoding in Egypt. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 12 e0007887 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Haiam Mohammed Mahmoud Farrag Enas Abdelhameed Mahmoud Huseein Amal M Almatary Ragaa A Othman Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Myiasis is the infestation of human tissues by dipterous fly larvae of the class Insecta. Clogmia albipunctatus, family Psychodidae, is one of the most medically important insects that cause human myiasis. The aim of the present study is the morphological identification and the molecular characterization of moth flies causing many cases of urinary myiasis in Egypt, based on sequencing of the mitochondrial DNA of the larvae. Seven urinary samples of patients complaining of urinary symptoms and giving a history of low socioeconomic level were examined. Recovered larvae were identified using light microscopy and SEM. For molecular identification, the mitochondrial genes Cytochrome B (cytB), NADH1, NADH1, and 16S were sequenced and phylogenetically analyzed. The morphological and molecular characterization could accurately diagnose our patients to have C. albipunctatus infestation. Such results provided the initial set of data on the molecular identification and phylogenetic analysis of moth flies based on DNA barcoding in Egypt. |
format |
Article in Journal/Newspaper |
author |
Haiam Mohammed Mahmoud Farrag Enas Abdelhameed Mahmoud Huseein Amal M Almatary Ragaa A Othman |
author_facet |
Haiam Mohammed Mahmoud Farrag Enas Abdelhameed Mahmoud Huseein Amal M Almatary Ragaa A Othman |
author_sort |
Haiam Mohammed Mahmoud Farrag |
title |
Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt. |
title_short |
Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt. |
title_full |
Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt. |
title_fullStr |
Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt. |
title_full_unstemmed |
Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt. |
title_sort |
morphological and initial molecular characterization of clogmia albipunctatus larvae (diptera: psychodidae) causing urinary myiasis in egypt. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007887 https://doaj.org/article/4dd15cee52c64435844f64322b3dabbe |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007887 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007887 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007887 https://doaj.org/article/4dd15cee52c64435844f64322b3dabbe |
op_doi |
https://doi.org/10.1371/journal.pntd.0007887 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
12 |
container_start_page |
e0007887 |
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1766335821273628672 |