Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes
Abstract The shells of window pane oyster Placuna placenta are very thin and exhibit excellent optical transparency and mechanical robustness. However, little is known about the biomineralization-related proteins of the shells of P. placenta. In this work, we report the comprehensive transcriptome o...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Portfolio
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1038/s41598-022-08610-5 https://doaj.org/article/4d9d3fa40ecb49d2bce3cd09623b5989 |
id |
ftdoajarticles:oai:doaj.org/article:4d9d3fa40ecb49d2bce3cd09623b5989 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:4d9d3fa40ecb49d2bce3cd09623b5989 2023-05-15T15:58:58+02:00 Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes Ningjing Song Jiangfeng Li Baosheng Li Ercai Pan Yurong Ma 2022-03-01T00:00:00Z https://doi.org/10.1038/s41598-022-08610-5 https://doaj.org/article/4d9d3fa40ecb49d2bce3cd09623b5989 EN eng Nature Portfolio https://doi.org/10.1038/s41598-022-08610-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-08610-5 2045-2322 https://doaj.org/article/4d9d3fa40ecb49d2bce3cd09623b5989 Scientific Reports, Vol 12, Iss 1, Pp 1-19 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1038/s41598-022-08610-5 2022-12-31T07:03:12Z Abstract The shells of window pane oyster Placuna placenta are very thin and exhibit excellent optical transparency and mechanical robustness. However, little is known about the biomineralization-related proteins of the shells of P. placenta. In this work, we report the comprehensive transcriptome of the mantle tissue of P. placenta for the first time. The unigenes of the mantle tissue of P. placenta were annotated by using the public databases such as nr, GO, KOG, KEGG, and Pfam. 24,343 unigenes were annotated according to Pfam database, accounting for 21.48% of the total unigenes. We find that half of the annotated unigenes of the mantle tissue of P. placenta are consistent to the annotated unigenes from pacific oyster Crassostrea gigas according to nr database. The unigene sequence analysis from the mantle tissue of P. placenta indicates that 465,392 potential single nucleotide polymorphisms (SNPs) and 62,103 potential indel markers were identified from 60,371 unigenes. 178 unigenes of the mantle tissue of P. placenta are found to be homologous to those reported proteins related to the biomineralization process of molluscan shells, while 18 of them are highly expressed unigenes in the mantle tissue. It is proposed that four unigenes with the highest expression levels in the mantle tissue are very often related to the biomineralization process, while another three unigenes are potentially related to the biomineralization process according to the Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) analysis. In summary, the transcriptome analysis of the mantle tissue of P. Placenta shows the potential biomineralization-related proteins and this work may shed light for the shell formation mechanism of bivalves. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Indel’ ENVELOPE(35.282,35.282,66.963,66.963) Pacific Scientific Reports 12 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Ningjing Song Jiangfeng Li Baosheng Li Ercai Pan Yurong Ma Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes |
topic_facet |
Medicine R Science Q |
description |
Abstract The shells of window pane oyster Placuna placenta are very thin and exhibit excellent optical transparency and mechanical robustness. However, little is known about the biomineralization-related proteins of the shells of P. placenta. In this work, we report the comprehensive transcriptome of the mantle tissue of P. placenta for the first time. The unigenes of the mantle tissue of P. placenta were annotated by using the public databases such as nr, GO, KOG, KEGG, and Pfam. 24,343 unigenes were annotated according to Pfam database, accounting for 21.48% of the total unigenes. We find that half of the annotated unigenes of the mantle tissue of P. placenta are consistent to the annotated unigenes from pacific oyster Crassostrea gigas according to nr database. The unigene sequence analysis from the mantle tissue of P. placenta indicates that 465,392 potential single nucleotide polymorphisms (SNPs) and 62,103 potential indel markers were identified from 60,371 unigenes. 178 unigenes of the mantle tissue of P. placenta are found to be homologous to those reported proteins related to the biomineralization process of molluscan shells, while 18 of them are highly expressed unigenes in the mantle tissue. It is proposed that four unigenes with the highest expression levels in the mantle tissue are very often related to the biomineralization process, while another three unigenes are potentially related to the biomineralization process according to the Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) analysis. In summary, the transcriptome analysis of the mantle tissue of P. Placenta shows the potential biomineralization-related proteins and this work may shed light for the shell formation mechanism of bivalves. |
format |
Article in Journal/Newspaper |
author |
Ningjing Song Jiangfeng Li Baosheng Li Ercai Pan Yurong Ma |
author_facet |
Ningjing Song Jiangfeng Li Baosheng Li Ercai Pan Yurong Ma |
author_sort |
Ningjing Song |
title |
Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes |
title_short |
Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes |
title_full |
Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes |
title_fullStr |
Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes |
title_full_unstemmed |
Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes |
title_sort |
transcriptome analysis of the bivalve placuna placenta mantle reveals potential biomineralization-related genes |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41598-022-08610-5 https://doaj.org/article/4d9d3fa40ecb49d2bce3cd09623b5989 |
long_lat |
ENVELOPE(35.282,35.282,66.963,66.963) |
geographic |
Indel’ Pacific |
geographic_facet |
Indel’ Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Scientific Reports, Vol 12, Iss 1, Pp 1-19 (2022) |
op_relation |
https://doi.org/10.1038/s41598-022-08610-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-08610-5 2045-2322 https://doaj.org/article/4d9d3fa40ecb49d2bce3cd09623b5989 |
op_doi |
https://doi.org/10.1038/s41598-022-08610-5 |
container_title |
Scientific Reports |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766394747747827712 |