Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal
Abstract Background Malaria surveillance requires powerful tools and strategies to achieve malaria elimination. Rapid diagnostic tests for malaria (RDTs) are easily deployed on a large scale and are helpful sources of parasite DNA. The application of sensitive molecular techniques to these RDTs is a...
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ftdoajarticles:oai:doaj.org/article:4d34de6173f34c23a3c15eb19ebb8864 2023-05-15T15:12:55+02:00 Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal Kiswendsida Thierry Guiguemde Yakou Dieye Aminata Collé Lô Magatte Ndiaye Aminata Lam Isaac Akhénaton Manga Gnagna Dieng Sow Moussa Diop Tamba Souané Marie Pièrre Diouf Roger Clément Kouly Tine Babacar Faye 2020-03-01T00:00:00Z https://doi.org/10.1186/s12936-020-03204-w https://doaj.org/article/4d34de6173f34c23a3c15eb19ebb8864 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03204-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03204-w 1475-2875 https://doaj.org/article/4d34de6173f34c23a3c15eb19ebb8864 Malaria Journal, Vol 19, Iss 1, Pp 1-7 (2020) Malaria RDT Gametocytes DNA extraction Quantification Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03204-w 2022-12-31T00:06:56Z Abstract Background Malaria surveillance requires powerful tools and strategies to achieve malaria elimination. Rapid diagnostic tests for malaria (RDTs) are easily deployed on a large scale and are helpful sources of parasite DNA. The application of sensitive molecular techniques to these RDTs is a modern tool for improving malaria case detection and drug resistance surveillance. Several studies have made it possible to extract the DNA of Plasmodium falciparum from RDTs. The knowledge of gametocyte carriage in the population is important to better assess the level of parasite transmission in elimination settings. The aim of this study was to detect P. falciparum gametocytes from used RDTs by quantitative PCR for molecular monitoring of malaria transmission. Methods DNA was extracted from 303 RDT devices (SD Bioline Malaria Pf) using the Chelex-100 protocol. qPCR was performed in a 20 μL reaction to detect and quantify transcripts of the pfs25 gene. The cycle threshold (Ct) was determined by the emission fluorescence corresponding to the initial amount of amplified DNA. Results The study found an overall prevalence of 53.47% with an average Ct of 32.12 ± 4.28 cycles. In 2018, the prevalence of gametocytes was higher in the Ranérou district (76.24%) than in the Saint-Louis district (67.33%) where an increase in the number of gametocyte carriers in 2018 was noted, in comparison with 2017. Conclusions RDTs are a good source of DNA for molecular monitoring of gametocyte carriage. This method is a simple and effective tool to better understand the level of malaria transmission with a view to elimination. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria RDT Gametocytes DNA extraction Quantification Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria RDT Gametocytes DNA extraction Quantification Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Kiswendsida Thierry Guiguemde Yakou Dieye Aminata Collé Lô Magatte Ndiaye Aminata Lam Isaac Akhénaton Manga Gnagna Dieng Sow Moussa Diop Tamba Souané Marie Pièrre Diouf Roger Clément Kouly Tine Babacar Faye Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal |
topic_facet |
Malaria RDT Gametocytes DNA extraction Quantification Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria surveillance requires powerful tools and strategies to achieve malaria elimination. Rapid diagnostic tests for malaria (RDTs) are easily deployed on a large scale and are helpful sources of parasite DNA. The application of sensitive molecular techniques to these RDTs is a modern tool for improving malaria case detection and drug resistance surveillance. Several studies have made it possible to extract the DNA of Plasmodium falciparum from RDTs. The knowledge of gametocyte carriage in the population is important to better assess the level of parasite transmission in elimination settings. The aim of this study was to detect P. falciparum gametocytes from used RDTs by quantitative PCR for molecular monitoring of malaria transmission. Methods DNA was extracted from 303 RDT devices (SD Bioline Malaria Pf) using the Chelex-100 protocol. qPCR was performed in a 20 μL reaction to detect and quantify transcripts of the pfs25 gene. The cycle threshold (Ct) was determined by the emission fluorescence corresponding to the initial amount of amplified DNA. Results The study found an overall prevalence of 53.47% with an average Ct of 32.12 ± 4.28 cycles. In 2018, the prevalence of gametocytes was higher in the Ranérou district (76.24%) than in the Saint-Louis district (67.33%) where an increase in the number of gametocyte carriers in 2018 was noted, in comparison with 2017. Conclusions RDTs are a good source of DNA for molecular monitoring of gametocyte carriage. This method is a simple and effective tool to better understand the level of malaria transmission with a view to elimination. |
format |
Article in Journal/Newspaper |
author |
Kiswendsida Thierry Guiguemde Yakou Dieye Aminata Collé Lô Magatte Ndiaye Aminata Lam Isaac Akhénaton Manga Gnagna Dieng Sow Moussa Diop Tamba Souané Marie Pièrre Diouf Roger Clément Kouly Tine Babacar Faye |
author_facet |
Kiswendsida Thierry Guiguemde Yakou Dieye Aminata Collé Lô Magatte Ndiaye Aminata Lam Isaac Akhénaton Manga Gnagna Dieng Sow Moussa Diop Tamba Souané Marie Pièrre Diouf Roger Clément Kouly Tine Babacar Faye |
author_sort |
Kiswendsida Thierry Guiguemde |
title |
Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal |
title_short |
Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal |
title_full |
Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal |
title_fullStr |
Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal |
title_full_unstemmed |
Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal |
title_sort |
molecular detection and quantification of plasmodium falciparum gametocytes carriage in used rdts in malaria elimination settings in northern senegal |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03204-w https://doaj.org/article/4d34de6173f34c23a3c15eb19ebb8864 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-7 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03204-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03204-w 1475-2875 https://doaj.org/article/4d34de6173f34c23a3c15eb19ebb8864 |
op_doi |
https://doi.org/10.1186/s12936-020-03204-w |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
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1766343538338955264 |