De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites
Abstract Understanding the genomic underpinnings of thermal adaptation is a hot topic in eco-evolutionary studies of parasites. Marine heteroxenous parasites have complex life cycles encompassing a free-living larval stage, an ectothermic intermediate host and a homeothermic definitive host, thus re...
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ftdoajarticles:oai:doaj.org/article:4d28a669bc0f46f880dd55e933f4589c 2023-11-12T04:08:42+01:00 De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites Marialetizia Palomba Pietro Libro Jessica Di Martino Xavier Roca-Geronès Armando Macali Tiziana Castrignanò Daniele Canestrelli Simonetta Mattiucci 2023-10-01T00:00:00Z https://doi.org/10.1038/s41597-023-02591-4 https://doaj.org/article/4d28a669bc0f46f880dd55e933f4589c EN eng Nature Portfolio https://doi.org/10.1038/s41597-023-02591-4 https://doaj.org/toc/2052-4463 doi:10.1038/s41597-023-02591-4 2052-4463 https://doaj.org/article/4d28a669bc0f46f880dd55e933f4589c Scientific Data, Vol 10, Iss 1, Pp 1-10 (2023) Science Q article 2023 ftdoajarticles https://doi.org/10.1038/s41597-023-02591-4 2023-10-29T00:42:10Z Abstract Understanding the genomic underpinnings of thermal adaptation is a hot topic in eco-evolutionary studies of parasites. Marine heteroxenous parasites have complex life cycles encompassing a free-living larval stage, an ectothermic intermediate host and a homeothermic definitive host, thus representing compelling systems for the study of thermal adaptation. The Antarctic anisakid Contracaecum osculatum sp. D is a marine parasite able to survive and thrive both at very cold and warm temperatures within the environment and its hosts. Here, a de novo transcriptome of C. osculatum sp. D was generated for the first time, by performing RNA-Seq experiments on a set of individuals exposed to temperatures experienced by the nematode during its life cycle. The analysis generated 425,954,724 reads, which were assembled and then annotated. The high-quality assembly was validated, achieving over 88% mapping against the transcriptome. The transcriptome of this parasite will represent a valuable genomic resource for future studies aimed at disentangling the genomic architecture of thermal tolerance and metabolic pathways related to temperature stress. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Scientific Data 10 1 |
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Science Q Marialetizia Palomba Pietro Libro Jessica Di Martino Xavier Roca-Geronès Armando Macali Tiziana Castrignanò Daniele Canestrelli Simonetta Mattiucci De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites |
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Abstract Understanding the genomic underpinnings of thermal adaptation is a hot topic in eco-evolutionary studies of parasites. Marine heteroxenous parasites have complex life cycles encompassing a free-living larval stage, an ectothermic intermediate host and a homeothermic definitive host, thus representing compelling systems for the study of thermal adaptation. The Antarctic anisakid Contracaecum osculatum sp. D is a marine parasite able to survive and thrive both at very cold and warm temperatures within the environment and its hosts. Here, a de novo transcriptome of C. osculatum sp. D was generated for the first time, by performing RNA-Seq experiments on a set of individuals exposed to temperatures experienced by the nematode during its life cycle. The analysis generated 425,954,724 reads, which were assembled and then annotated. The high-quality assembly was validated, achieving over 88% mapping against the transcriptome. The transcriptome of this parasite will represent a valuable genomic resource for future studies aimed at disentangling the genomic architecture of thermal tolerance and metabolic pathways related to temperature stress. |
format |
Article in Journal/Newspaper |
author |
Marialetizia Palomba Pietro Libro Jessica Di Martino Xavier Roca-Geronès Armando Macali Tiziana Castrignanò Daniele Canestrelli Simonetta Mattiucci |
author_facet |
Marialetizia Palomba Pietro Libro Jessica Di Martino Xavier Roca-Geronès Armando Macali Tiziana Castrignanò Daniele Canestrelli Simonetta Mattiucci |
author_sort |
Marialetizia Palomba |
title |
De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites |
title_short |
De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites |
title_full |
De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites |
title_fullStr |
De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites |
title_full_unstemmed |
De novo transcriptome assembly of an Antarctic nematode for the study of thermal adaptation in marine parasites |
title_sort |
de novo transcriptome assembly of an antarctic nematode for the study of thermal adaptation in marine parasites |
publisher |
Nature Portfolio |
publishDate |
2023 |
url |
https://doi.org/10.1038/s41597-023-02591-4 https://doaj.org/article/4d28a669bc0f46f880dd55e933f4589c |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Scientific Data, Vol 10, Iss 1, Pp 1-10 (2023) |
op_relation |
https://doi.org/10.1038/s41597-023-02591-4 https://doaj.org/toc/2052-4463 doi:10.1038/s41597-023-02591-4 2052-4463 https://doaj.org/article/4d28a669bc0f46f880dd55e933f4589c |
op_doi |
https://doi.org/10.1038/s41597-023-02591-4 |
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Scientific Data |
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10 |
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1 |
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1782328926020829184 |