Analysis of innate defences against Plasmodium falciparum in immunodeficient mice
Abstract Background Mice with genetic deficiencies in adaptive immunity are used for the grafting of human cells or pathogens, to study human diseases, however, the innate immune responses to xenografts in these mice has received little attention. Using the NOD/SCID Plasmodium falciparum mouse model...
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ftdoajarticles:oai:doaj.org/article:4d037727bb4042eca1b7472c954b1a46 2023-05-15T15:13:36+02:00 Analysis of innate defences against Plasmodium falciparum in immunodeficient mice Van Rooijen Nico Mejia Pedro Tyagi Rajeev Arnold Ludovic Pérignon Jean-Louis Druilhe Pierre 2010-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-197 https://doaj.org/article/4d037727bb4042eca1b7472c954b1a46 EN eng BMC http://www.malariajournal.com/content/9/1/197 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-197 1475-2875 https://doaj.org/article/4d037727bb4042eca1b7472c954b1a46 Malaria Journal, Vol 9, Iss 1, p 197 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-197 2022-12-31T01:42:30Z Abstract Background Mice with genetic deficiencies in adaptive immunity are used for the grafting of human cells or pathogens, to study human diseases, however, the innate immune responses to xenografts in these mice has received little attention. Using the NOD/SCID Plasmodium falciparum mouse model an analysis of innate defences responsible for the substantial control of P. falciparum which remains in such mice, was performed. Methods NOD/SCID mice undergoing an immunomodulatory protocol that includes, clodronate-loaded liposomes to deplete macrophages and an anti-polymorphonuclear leukocytes antibody, were grafted with human red blood cells and P. falciparum . The systematic and kinetic analysis of the remaining innate immune responses included the number and phenotype of peripheral blood leukocytes as well as inflammatory cytokines/chemokines released in periphery. The innate responses towards the murine parasite Plasmodium yoelii were used as a control. Results Results show that 1) P. falciparum induces a strong inflammation characterized by an increase in circulating leukocytes and the release of inflammatory cytokines; 2) in contrast, the rodent parasite P. yoelii , induces a far more moderate inflammation; 3) human red blood cells and the anti-inflammatory agents employed induce low-grade inflammation; and 4) macrophages seem to bear the most critical function in controlling P. falciparum survival in those mice, whereas polymorphonuclear and NK cells have only a minor role. Conclusions Despite the use of an immunomodulatory treatment, immunodeficient NOD/SCID mice are still able to mount substantial innate responses that seem to be correlated with parasite clearance. Those results bring new insights on the ability of innate immunity from immunodeficient mice to control xenografts of cells of human origin and human pathogens. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 197 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Van Rooijen Nico Mejia Pedro Tyagi Rajeev Arnold Ludovic Pérignon Jean-Louis Druilhe Pierre Analysis of innate defences against Plasmodium falciparum in immunodeficient mice |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Mice with genetic deficiencies in adaptive immunity are used for the grafting of human cells or pathogens, to study human diseases, however, the innate immune responses to xenografts in these mice has received little attention. Using the NOD/SCID Plasmodium falciparum mouse model an analysis of innate defences responsible for the substantial control of P. falciparum which remains in such mice, was performed. Methods NOD/SCID mice undergoing an immunomodulatory protocol that includes, clodronate-loaded liposomes to deplete macrophages and an anti-polymorphonuclear leukocytes antibody, were grafted with human red blood cells and P. falciparum . The systematic and kinetic analysis of the remaining innate immune responses included the number and phenotype of peripheral blood leukocytes as well as inflammatory cytokines/chemokines released in periphery. The innate responses towards the murine parasite Plasmodium yoelii were used as a control. Results Results show that 1) P. falciparum induces a strong inflammation characterized by an increase in circulating leukocytes and the release of inflammatory cytokines; 2) in contrast, the rodent parasite P. yoelii , induces a far more moderate inflammation; 3) human red blood cells and the anti-inflammatory agents employed induce low-grade inflammation; and 4) macrophages seem to bear the most critical function in controlling P. falciparum survival in those mice, whereas polymorphonuclear and NK cells have only a minor role. Conclusions Despite the use of an immunomodulatory treatment, immunodeficient NOD/SCID mice are still able to mount substantial innate responses that seem to be correlated with parasite clearance. Those results bring new insights on the ability of innate immunity from immunodeficient mice to control xenografts of cells of human origin and human pathogens. |
format |
Article in Journal/Newspaper |
author |
Van Rooijen Nico Mejia Pedro Tyagi Rajeev Arnold Ludovic Pérignon Jean-Louis Druilhe Pierre |
author_facet |
Van Rooijen Nico Mejia Pedro Tyagi Rajeev Arnold Ludovic Pérignon Jean-Louis Druilhe Pierre |
author_sort |
Van Rooijen Nico |
title |
Analysis of innate defences against Plasmodium falciparum in immunodeficient mice |
title_short |
Analysis of innate defences against Plasmodium falciparum in immunodeficient mice |
title_full |
Analysis of innate defences against Plasmodium falciparum in immunodeficient mice |
title_fullStr |
Analysis of innate defences against Plasmodium falciparum in immunodeficient mice |
title_full_unstemmed |
Analysis of innate defences against Plasmodium falciparum in immunodeficient mice |
title_sort |
analysis of innate defences against plasmodium falciparum in immunodeficient mice |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-197 https://doaj.org/article/4d037727bb4042eca1b7472c954b1a46 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 197 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/197 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-197 1475-2875 https://doaj.org/article/4d037727bb4042eca1b7472c954b1a46 |
op_doi |
https://doi.org/10.1186/1475-2875-9-197 |
container_title |
Malaria Journal |
container_volume |
9 |
container_issue |
1 |
container_start_page |
197 |
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1766344137672491008 |