DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
Abstract We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m3 of air were pumped through a 0.22 µm membrane. Snow obtained shortly after depositi...
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ftdoajarticles:oai:doaj.org/article:4cf6e031d4464d74a37024887aef92ae 2023-05-15T13:59:54+02:00 DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica Luiz Henrique Rosa Otávio Henrique Bezerra Pinto Tina Šantl-Temkiv Peter Convey Micheline Carvalho-Silva Carlos Augusto Rosa Paulo E. A. S. Câmara 2020-12-01T00:00:00Z https://doi.org/10.1038/s41598-020-78630-6 https://doaj.org/article/4cf6e031d4464d74a37024887aef92ae EN eng Nature Portfolio https://doi.org/10.1038/s41598-020-78630-6 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-020-78630-6 2045-2322 https://doaj.org/article/4cf6e031d4464d74a37024887aef92ae Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Medicine R Science Q article 2020 ftdoajarticles https://doi.org/10.1038/s41598-020-78630-6 2022-12-31T05:24:32Z Abstract We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m3 of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was kept at room temperature and yielded 3.760 L of water, which was filtered using Sterivex membranes of 0.22 µm mesh size. The total DNA present was extracted and sequenced. We detected 171 fungal amplicon sequence variants (ASVs), 70 from the air and 142 from the snow. They were dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Mucoromycota. Pseudogymnoascus, Cladosporium, Mortierella and Penicillium sp. were the most dominant ASVs detected in the air in rank order. In snow, Cladosporium, Pseudogymnoascus, Penicillium, Meyerozyma, Lecidea, Malassezia, Hanseniaspora, Austroplaca, Mortierella, Rhodotorula, Penicillium, Thelebolus, Aspergillus, Poaceicola, Glarea and Lecanora were the dominant ASVs present. In general, the two fungal assemblages displayed high diversity, richness, and dominance indices, with the assemblage found in snow having the highest diversity indices. Of the total fungal ASVs detected, 29 were only present in the air sample and 101 in the snow sample, with only 41 present in both samples; however, when only the dominant taxa from both samples were compared none occurred only in the air and, among the rare portion, 26 taxa occurred in both air and snow. Application of HTS revealed the presence of a more diverse fungal community in the air and snow of Livingston Island in comparison with studies using traditional isolation methods. The assemblages were dominated by cold-adapted and cosmopolitan fungal taxa, including members of the genera Pseudogymnoascus, Malassezia and Rhodotorula, which include some taxa reported as opportunistic. Our results support the hypothesis that the presence of microbiota in the airspora indicates the possibility of dispersal around Antarctica ... Article in Journal/Newspaper Antarc* Antarctica Livingston Island South Shetland Islands Directory of Open Access Journals: DOAJ Articles South Shetland Islands Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Scientific Reports 10 1 |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Luiz Henrique Rosa Otávio Henrique Bezerra Pinto Tina Šantl-Temkiv Peter Convey Micheline Carvalho-Silva Carlos Augusto Rosa Paulo E. A. S. Câmara DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
topic_facet |
Medicine R Science Q |
description |
Abstract We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m3 of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was kept at room temperature and yielded 3.760 L of water, which was filtered using Sterivex membranes of 0.22 µm mesh size. The total DNA present was extracted and sequenced. We detected 171 fungal amplicon sequence variants (ASVs), 70 from the air and 142 from the snow. They were dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Mucoromycota. Pseudogymnoascus, Cladosporium, Mortierella and Penicillium sp. were the most dominant ASVs detected in the air in rank order. In snow, Cladosporium, Pseudogymnoascus, Penicillium, Meyerozyma, Lecidea, Malassezia, Hanseniaspora, Austroplaca, Mortierella, Rhodotorula, Penicillium, Thelebolus, Aspergillus, Poaceicola, Glarea and Lecanora were the dominant ASVs present. In general, the two fungal assemblages displayed high diversity, richness, and dominance indices, with the assemblage found in snow having the highest diversity indices. Of the total fungal ASVs detected, 29 were only present in the air sample and 101 in the snow sample, with only 41 present in both samples; however, when only the dominant taxa from both samples were compared none occurred only in the air and, among the rare portion, 26 taxa occurred in both air and snow. Application of HTS revealed the presence of a more diverse fungal community in the air and snow of Livingston Island in comparison with studies using traditional isolation methods. The assemblages were dominated by cold-adapted and cosmopolitan fungal taxa, including members of the genera Pseudogymnoascus, Malassezia and Rhodotorula, which include some taxa reported as opportunistic. Our results support the hypothesis that the presence of microbiota in the airspora indicates the possibility of dispersal around Antarctica ... |
format |
Article in Journal/Newspaper |
author |
Luiz Henrique Rosa Otávio Henrique Bezerra Pinto Tina Šantl-Temkiv Peter Convey Micheline Carvalho-Silva Carlos Augusto Rosa Paulo E. A. S. Câmara |
author_facet |
Luiz Henrique Rosa Otávio Henrique Bezerra Pinto Tina Šantl-Temkiv Peter Convey Micheline Carvalho-Silva Carlos Augusto Rosa Paulo E. A. S. Câmara |
author_sort |
Luiz Henrique Rosa |
title |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_short |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_full |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_fullStr |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_full_unstemmed |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_sort |
dna metabarcoding of fungal diversity in air and snow of livingston island, south shetland islands, antarctica |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doi.org/10.1038/s41598-020-78630-6 https://doaj.org/article/4cf6e031d4464d74a37024887aef92ae |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
South Shetland Islands Livingston Island |
geographic_facet |
South Shetland Islands Livingston Island |
genre |
Antarc* Antarctica Livingston Island South Shetland Islands |
genre_facet |
Antarc* Antarctica Livingston Island South Shetland Islands |
op_source |
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
op_relation |
https://doi.org/10.1038/s41598-020-78630-6 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-020-78630-6 2045-2322 https://doaj.org/article/4cf6e031d4464d74a37024887aef92ae |
op_doi |
https://doi.org/10.1038/s41598-020-78630-6 |
container_title |
Scientific Reports |
container_volume |
10 |
container_issue |
1 |
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1766268831712411648 |