qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records
Lake sediment serves as a natural archive of historical biological information. The use of sedimentary DNA (sedDNA), a form of environmental DNA (eDNA) shed by aquatic organisms and preserved in sediment, has been instrumental in reconstructing past faunal composition in aquatic communities. However...
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2023
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Online Access: | https://doi.org/10.1016/j.ecolind.2023.111014 https://doaj.org/article/4cf0eac8de24409fae52fb6da696f54e |
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ftdoajarticles:oai:doaj.org/article:4cf0eac8de24409fae52fb6da696f54e 2023-11-12T04:15:52+01:00 qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records Mark Louie D. Lopez Matthew Bonderud Michael J. Allison Findlay MacDermid Erin J. Ussery Mark E. McMaster Ave Dersch Kasia J. Staniszewska Colin A. Cooke Paul Drevnick Caren C. Helbing 2023-11-01T00:00:00Z https://doi.org/10.1016/j.ecolind.2023.111014 https://doaj.org/article/4cf0eac8de24409fae52fb6da696f54e EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1470160X23011561 https://doaj.org/toc/1470-160X 1470-160X doi:10.1016/j.ecolind.2023.111014 https://doaj.org/article/4cf0eac8de24409fae52fb6da696f54e Ecological Indicators, Vol 155, Iss , Pp 111014- (2023) Fish fauna eDNA assay environmental DNA PCR inhibitors First Nations knowledge Historical biodiversity reconstruction Ecology QH540-549.5 article 2023 ftdoajarticles https://doi.org/10.1016/j.ecolind.2023.111014 2023-10-22T00:36:36Z Lake sediment serves as a natural archive of historical biological information. The use of sedimentary DNA (sedDNA), a form of environmental DNA (eDNA) shed by aquatic organisms and preserved in sediment, has been instrumental in reconstructing past faunal composition in aquatic communities. However, the low abundance of fish sedDNA and the often humic-rich nature of lake sediments create methodological challenges for the accurate detection of target sedDNA using quantitative polymerase chain reaction (qPCR)-based approaches. Herein, we present a consolidated qPCR-based eDNA workflow to reconstruct past and current fish fauna in Cowpar Lake located in the Oil Sands region in Alberta (Canada), which were then validated using Indigenous Knowledge from Chipewyan Prairie First Nation community members. The present study highlights the importance of combining column- and precipitation-based PCR inhibitor clean-up, nucleic acid concentration, incorporating endogenous chloroplast DNA as a sample integrity control. Robust qPCR-based eDNA assays were also useful in preventing the false-negative detection of low copies of target fish DNA. The presence of Northern pike (1905 to 2019) and Cisco (1919 to 1942) in Cowpar Lake was confirmed based on detected sedDNA from sediment core. The reconstructed fish records from sedDNA-inferred data aligned with the Indigenous accounts of natural and human-mediated changes in land use around the lake. Overall, the present study addresses common methodological concerns in processing lake sediment samples for fish eDNA detection and demonstrates the great potential of combined eDNA-inferred data and Indigenous Knowledge in reconstructing historical fish records in aquatic communities. Article in Journal/Newspaper Chipewyan First Nations Directory of Open Access Journals: DOAJ Articles Canada Ecological Indicators 155 111014 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Fish fauna eDNA assay environmental DNA PCR inhibitors First Nations knowledge Historical biodiversity reconstruction Ecology QH540-549.5 |
spellingShingle |
Fish fauna eDNA assay environmental DNA PCR inhibitors First Nations knowledge Historical biodiversity reconstruction Ecology QH540-549.5 Mark Louie D. Lopez Matthew Bonderud Michael J. Allison Findlay MacDermid Erin J. Ussery Mark E. McMaster Ave Dersch Kasia J. Staniszewska Colin A. Cooke Paul Drevnick Caren C. Helbing qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records |
topic_facet |
Fish fauna eDNA assay environmental DNA PCR inhibitors First Nations knowledge Historical biodiversity reconstruction Ecology QH540-549.5 |
description |
Lake sediment serves as a natural archive of historical biological information. The use of sedimentary DNA (sedDNA), a form of environmental DNA (eDNA) shed by aquatic organisms and preserved in sediment, has been instrumental in reconstructing past faunal composition in aquatic communities. However, the low abundance of fish sedDNA and the often humic-rich nature of lake sediments create methodological challenges for the accurate detection of target sedDNA using quantitative polymerase chain reaction (qPCR)-based approaches. Herein, we present a consolidated qPCR-based eDNA workflow to reconstruct past and current fish fauna in Cowpar Lake located in the Oil Sands region in Alberta (Canada), which were then validated using Indigenous Knowledge from Chipewyan Prairie First Nation community members. The present study highlights the importance of combining column- and precipitation-based PCR inhibitor clean-up, nucleic acid concentration, incorporating endogenous chloroplast DNA as a sample integrity control. Robust qPCR-based eDNA assays were also useful in preventing the false-negative detection of low copies of target fish DNA. The presence of Northern pike (1905 to 2019) and Cisco (1919 to 1942) in Cowpar Lake was confirmed based on detected sedDNA from sediment core. The reconstructed fish records from sedDNA-inferred data aligned with the Indigenous accounts of natural and human-mediated changes in land use around the lake. Overall, the present study addresses common methodological concerns in processing lake sediment samples for fish eDNA detection and demonstrates the great potential of combined eDNA-inferred data and Indigenous Knowledge in reconstructing historical fish records in aquatic communities. |
format |
Article in Journal/Newspaper |
author |
Mark Louie D. Lopez Matthew Bonderud Michael J. Allison Findlay MacDermid Erin J. Ussery Mark E. McMaster Ave Dersch Kasia J. Staniszewska Colin A. Cooke Paul Drevnick Caren C. Helbing |
author_facet |
Mark Louie D. Lopez Matthew Bonderud Michael J. Allison Findlay MacDermid Erin J. Ussery Mark E. McMaster Ave Dersch Kasia J. Staniszewska Colin A. Cooke Paul Drevnick Caren C. Helbing |
author_sort |
Mark Louie D. Lopez |
title |
qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records |
title_short |
qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records |
title_full |
qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records |
title_fullStr |
qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records |
title_full_unstemmed |
qPCR-based eDNA workflow for humic-rich lake sediments: Combined use of sedimentary DNA (sedDNA) and Indigenous Knowledge in reconstructing historical fish records |
title_sort |
qpcr-based edna workflow for humic-rich lake sediments: combined use of sedimentary dna (seddna) and indigenous knowledge in reconstructing historical fish records |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://doi.org/10.1016/j.ecolind.2023.111014 https://doaj.org/article/4cf0eac8de24409fae52fb6da696f54e |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Chipewyan First Nations |
genre_facet |
Chipewyan First Nations |
op_source |
Ecological Indicators, Vol 155, Iss , Pp 111014- (2023) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1470160X23011561 https://doaj.org/toc/1470-160X 1470-160X doi:10.1016/j.ecolind.2023.111014 https://doaj.org/article/4cf0eac8de24409fae52fb6da696f54e |
op_doi |
https://doi.org/10.1016/j.ecolind.2023.111014 |
container_title |
Ecological Indicators |
container_volume |
155 |
container_start_page |
111014 |
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1782333116568829952 |