Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis.
Leptospirosis is a re-emerging tropical infectious disease caused by pathogenic Leptospira spp. The different host innate immune responses are partially related to the different severities of leptospirosis. In this study, we employed transcriptomics and cytokine arrays to comparatively calculate the...
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ftdoajarticles:oai:doaj.org/article:4ceef98e8296467597e19760aba879c0 2023-05-15T15:12:43+02:00 Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. Feng Xue Xinghui Zhao Yingchao Yang Jinping Zhao Yutao Yang Yongguo Cao Cailing Hong Yuan Liu Lan Sun Minjun Huang Junchao Gu 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002477 https://doaj.org/article/4ceef98e8296467597e19760aba879c0 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3794915?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002477 https://doaj.org/article/4ceef98e8296467597e19760aba879c0 PLoS Neglected Tropical Diseases, Vol 7, Iss 10, p e2477 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002477 2022-12-31T12:40:38Z Leptospirosis is a re-emerging tropical infectious disease caused by pathogenic Leptospira spp. The different host innate immune responses are partially related to the different severities of leptospirosis. In this study, we employed transcriptomics and cytokine arrays to comparatively calculate the responses of murine peritoneal macrophages (MPMs) and human peripheral blood monocytes (HBMs) to leptospiral infection. We uncovered a series of different expression profiles of these two immune cells. The percentages of regulated genes in several biological processes of MPMs, such as antigen processing and presentation, membrane potential regulation, and the innate immune response, etc., were much greater than those of HBMs (>2-fold). In MPMs and HBMs, the caspase-8 and Fas-associated protein with death domain (FADD)-like apoptosis regulator genes were significantly up-regulated, which supported previous results that the caspase-8 and caspase-3 pathways play an important role in macrophage apoptosis during leptospiral infection. In addition, the key component of the complement pathway, C3, was only up-regulated in MPMs. Furthermore, several cytokines, e.g. interleukin 10 (IL-10) and tumor necrosis factor alpha (TNF-alpha), were differentially expressed at both mRNA and protein levels in MPMs and HBMs. Some of the differential expressions were proved to be pathogenic Leptospira-specific regulations at mRNA level or protein level. Though it is still unclear why some animals are resistant and others are susceptible to leptospiral infection, this comparative study based on transcriptomics and cytokine arrays partially uncovered the differences of murine resistance and human susceptibility to leptospirosis. Taken together, these findings will facilitate further molecular studies on the innate immune response to leptospiral infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 10 e2477 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Feng Xue Xinghui Zhao Yingchao Yang Jinping Zhao Yutao Yang Yongguo Cao Cailing Hong Yuan Liu Lan Sun Minjun Huang Junchao Gu Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leptospirosis is a re-emerging tropical infectious disease caused by pathogenic Leptospira spp. The different host innate immune responses are partially related to the different severities of leptospirosis. In this study, we employed transcriptomics and cytokine arrays to comparatively calculate the responses of murine peritoneal macrophages (MPMs) and human peripheral blood monocytes (HBMs) to leptospiral infection. We uncovered a series of different expression profiles of these two immune cells. The percentages of regulated genes in several biological processes of MPMs, such as antigen processing and presentation, membrane potential regulation, and the innate immune response, etc., were much greater than those of HBMs (>2-fold). In MPMs and HBMs, the caspase-8 and Fas-associated protein with death domain (FADD)-like apoptosis regulator genes were significantly up-regulated, which supported previous results that the caspase-8 and caspase-3 pathways play an important role in macrophage apoptosis during leptospiral infection. In addition, the key component of the complement pathway, C3, was only up-regulated in MPMs. Furthermore, several cytokines, e.g. interleukin 10 (IL-10) and tumor necrosis factor alpha (TNF-alpha), were differentially expressed at both mRNA and protein levels in MPMs and HBMs. Some of the differential expressions were proved to be pathogenic Leptospira-specific regulations at mRNA level or protein level. Though it is still unclear why some animals are resistant and others are susceptible to leptospiral infection, this comparative study based on transcriptomics and cytokine arrays partially uncovered the differences of murine resistance and human susceptibility to leptospirosis. Taken together, these findings will facilitate further molecular studies on the innate immune response to leptospiral infection. |
format |
Article in Journal/Newspaper |
author |
Feng Xue Xinghui Zhao Yingchao Yang Jinping Zhao Yutao Yang Yongguo Cao Cailing Hong Yuan Liu Lan Sun Minjun Huang Junchao Gu |
author_facet |
Feng Xue Xinghui Zhao Yingchao Yang Jinping Zhao Yutao Yang Yongguo Cao Cailing Hong Yuan Liu Lan Sun Minjun Huang Junchao Gu |
author_sort |
Feng Xue |
title |
Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. |
title_short |
Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. |
title_full |
Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. |
title_fullStr |
Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. |
title_full_unstemmed |
Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. |
title_sort |
responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002477 https://doaj.org/article/4ceef98e8296467597e19760aba879c0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 10, p e2477 (2013) |
op_relation |
http://europepmc.org/articles/PMC3794915?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002477 https://doaj.org/article/4ceef98e8296467597e19760aba879c0 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002477 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
10 |
container_start_page |
e2477 |
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1766343360005537792 |