Trypanosoma cruzi and experimental Chagas' disease: characterization of a stock isolated from a patient with associated digestive and cardiac form

• Main Authors: E.C. Oliveira, M.M.A. Stefani, A.O. Luquetti, E.F. Vêncio, M.A.R. Moreira, C. Souza, J.M. Rezende
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 1993
• Subjects: Cepa de Trypanosoma cruzi; Doença de Chagas; Infecção experimental; Sorologia; Histopatologia; Trypanosoma cruzi strain; Chagas'disease; Experimental infection; Serology; Histopathology; Arctic medicine. Tropical medicine; RC955-962; Arctic; Aguda
• Online Access: https://doaj.org/article/4cd8d4da9c2146858356e4c6dc1d9285

A new Trypanosoma cruzi stock isolated from a patient in the chronic phase of Chagas' disease with the digestive and cardiac fortn of the disease was characterized by experimental infection in isogenic, susceptible, A/Sn strain mice. Parasitemia curves showed up to 1.7x10(6) parasites/ml and no mortality was observed up to 300 days post infection. Specific IgM was found in mice in the acute phase up to 40 days and also in the chronic phase. IgG antibodies yvere detected in the acute and chronic phase. Histopathology examination demonstrated myotropism to the digestive tract muscle layers and to the heart. Um novo estoque de Trypanosoma cruzi isolado de paciente chagásico crônico, com a forma digestiva e cardiaca da doença, foi caracterizado através de infecção experimental em camundongos isogênicosA/Sn suscetíveis à infecção chagásica. As curvas de parasitemia mostraram picos de até 1,7x10(6) parasitas/ml não se observando mortalidade até 300 dias pós infecção. Anticorpos da classe IgM foram encontrados na fase aguda até 40 dias e também na fase crônica e IgG foi detectada nas fases aguda e crônica. O exame histopatológico mostrou miotropismo para músculo liso do tubo digestivo e cardíaco.