Purification and biological effects of a C-type lectin isolated from Bothrops moojeni
Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and i...
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ftdoajarticles:oai:doaj.org/article:4cb7eff92c314b5e810099eb0ddc0e41 2023-05-15T15:09:44+02:00 Purification and biological effects of a C-type lectin isolated from Bothrops moojeni PSF Barbosa AMC Martins MH Toyama PP Joazeiro LOS Beriam MC Fonteles HSA Monteiro 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000300016 https://doaj.org/article/4cb7eff92c314b5e810099eb0ddc0e41 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300016 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300016 1678-9199 https://doaj.org/article/4cb7eff92c314b5e810099eb0ddc0e41 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 493-504 (2010) Bothrops moojeni kidney platelet aggregation insulin antibacterial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000300016 2022-12-31T14:56:08Z Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis pv. passiflorae treated with lectin disclosed a high vesiculation and membrane rupture. BmLec induced a strong and significant increase in insulin secretion at 2.8 and 16.7 mM glucose concentrations, and this effect was seen in the presence of EGTA in both experiments. BmLec (10 µg/mL) increased the perfusion pressure, renal vascular resistance and urinary flow. The glomerular filtration rate and percentages of sodium, potassium and chloride tubular transport were reduced at 60 minutes of perfusion. Renal alterations caused by BmLec were completely inhibited by indomethacin in all evaluated parameters. In conclusion, the C-type lectin isolated from Bothrops moojeni affected platelet aggregation, insulin secretion, antibacterial activity and isolated kidney function. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 3 493 504 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bothrops moojeni kidney platelet aggregation insulin antibacterial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Bothrops moojeni kidney platelet aggregation insulin antibacterial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 PSF Barbosa AMC Martins MH Toyama PP Joazeiro LOS Beriam MC Fonteles HSA Monteiro Purification and biological effects of a C-type lectin isolated from Bothrops moojeni |
topic_facet |
Bothrops moojeni kidney platelet aggregation insulin antibacterial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis pv. passiflorae treated with lectin disclosed a high vesiculation and membrane rupture. BmLec induced a strong and significant increase in insulin secretion at 2.8 and 16.7 mM glucose concentrations, and this effect was seen in the presence of EGTA in both experiments. BmLec (10 µg/mL) increased the perfusion pressure, renal vascular resistance and urinary flow. The glomerular filtration rate and percentages of sodium, potassium and chloride tubular transport were reduced at 60 minutes of perfusion. Renal alterations caused by BmLec were completely inhibited by indomethacin in all evaluated parameters. In conclusion, the C-type lectin isolated from Bothrops moojeni affected platelet aggregation, insulin secretion, antibacterial activity and isolated kidney function. |
format |
Article in Journal/Newspaper |
author |
PSF Barbosa AMC Martins MH Toyama PP Joazeiro LOS Beriam MC Fonteles HSA Monteiro |
author_facet |
PSF Barbosa AMC Martins MH Toyama PP Joazeiro LOS Beriam MC Fonteles HSA Monteiro |
author_sort |
PSF Barbosa |
title |
Purification and biological effects of a C-type lectin isolated from Bothrops moojeni |
title_short |
Purification and biological effects of a C-type lectin isolated from Bothrops moojeni |
title_full |
Purification and biological effects of a C-type lectin isolated from Bothrops moojeni |
title_fullStr |
Purification and biological effects of a C-type lectin isolated from Bothrops moojeni |
title_full_unstemmed |
Purification and biological effects of a C-type lectin isolated from Bothrops moojeni |
title_sort |
purification and biological effects of a c-type lectin isolated from bothrops moojeni |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000300016 https://doaj.org/article/4cb7eff92c314b5e810099eb0ddc0e41 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 493-504 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300016 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300016 1678-9199 https://doaj.org/article/4cb7eff92c314b5e810099eb0ddc0e41 |
op_doi |
https://doi.org/10.1590/S1678-91992010000300016 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
16 |
container_issue |
3 |
container_start_page |
493 |
op_container_end_page |
504 |
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1766340867207987200 |