Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice
Abstract Background Malaria is a worldwide problem that affects millions of people yearly. In rural areas where anti-malarial drugs are not easily accessible, many people use herbal treatments, such as Moringa oleifera, to treat a variety of diseases and ailments including malaria. While Moringa is...
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ftdoajarticles:oai:doaj.org/article:4cae3cd7a49143c9be2bf46e5e6700c8 2023-05-15T15:17:21+02:00 Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice Jennifer Pilotos Kadra Abdu Ibrahim Chishimba Nathan Mowa Michael Makokha Opata 2020-02-01T00:00:00Z https://doi.org/10.1186/s12936-020-3129-8 https://doaj.org/article/4cae3cd7a49143c9be2bf46e5e6700c8 EN eng BMC https://doi.org/10.1186/s12936-020-3129-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-3129-8 1475-2875 https://doaj.org/article/4cae3cd7a49143c9be2bf46e5e6700c8 Malaria Journal, Vol 19, Iss 1, Pp 1-16 (2020) Malaria Immunity Effector T cells Moringa Malnutrition Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-3129-8 2022-12-31T04:53:45Z Abstract Background Malaria is a worldwide problem that affects millions of people yearly. In rural areas where anti-malarial drugs are not easily accessible, many people use herbal treatments, such as Moringa oleifera, to treat a variety of diseases and ailments including malaria. While Moringa is reported to possess potent and curative anti-malarial properties, previous studies have mostly been restricted to assessment of parasitaemia. In this study, the effect of Moringa on malaria immunity in a murine model was investigated. Methods Using a high dose (60 mg/mouse) for a short time (7 days) or low dose Moringa (30 mg/mouse) for a longer time (3 weeks), cytokine production, and Tbet expression by effector CD4+ T cells (Teff) were determined. Mice were also treated with Moringa after infection (curatively) or before infection (prophylactically) to determine the effect of the plant extract on parasitaemia and immunity. Given that Moringa also possess many nutritional benefits, the contribution of Moringa on malnourished malaria infected mice was determined. Malnutrition was induced by limiting access to food to only 4 h a day for 4 weeks, while control mice had unlimited access to mouse laboratory chow. All data was collected by flow cytometry and analysed using one-Way ANOVA or two tailed Student’s t test. Results Moringa-treated mice had increased numbers of effector CD4+ T cells accompanied by an increase in Tbet expression compared to control untreated mice. Mice that were treated with Moringa curatively also exhibited increased effector CD4+ T cell numbers, IFN-gamma and TNF secretion. Interestingly, the mice that were treated prophylactically had significantly higher Tbet expression. In the absence of adaptive immunity, high parasitaemia was observed in the RAG1 knockout mice. The food limited mice (malnourished) had reduced numbers of CD4+ T cells, TNF proportions, and significantly greater Tbet expression compared to the control group. Supplementation with Moringa in the limited group slightly restored ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Immunity Effector T cells Moringa Malnutrition Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Immunity Effector T cells Moringa Malnutrition Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jennifer Pilotos Kadra Abdu Ibrahim Chishimba Nathan Mowa Michael Makokha Opata Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice |
topic_facet |
Malaria Immunity Effector T cells Moringa Malnutrition Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is a worldwide problem that affects millions of people yearly. In rural areas where anti-malarial drugs are not easily accessible, many people use herbal treatments, such as Moringa oleifera, to treat a variety of diseases and ailments including malaria. While Moringa is reported to possess potent and curative anti-malarial properties, previous studies have mostly been restricted to assessment of parasitaemia. In this study, the effect of Moringa on malaria immunity in a murine model was investigated. Methods Using a high dose (60 mg/mouse) for a short time (7 days) or low dose Moringa (30 mg/mouse) for a longer time (3 weeks), cytokine production, and Tbet expression by effector CD4+ T cells (Teff) were determined. Mice were also treated with Moringa after infection (curatively) or before infection (prophylactically) to determine the effect of the plant extract on parasitaemia and immunity. Given that Moringa also possess many nutritional benefits, the contribution of Moringa on malnourished malaria infected mice was determined. Malnutrition was induced by limiting access to food to only 4 h a day for 4 weeks, while control mice had unlimited access to mouse laboratory chow. All data was collected by flow cytometry and analysed using one-Way ANOVA or two tailed Student’s t test. Results Moringa-treated mice had increased numbers of effector CD4+ T cells accompanied by an increase in Tbet expression compared to control untreated mice. Mice that were treated with Moringa curatively also exhibited increased effector CD4+ T cell numbers, IFN-gamma and TNF secretion. Interestingly, the mice that were treated prophylactically had significantly higher Tbet expression. In the absence of adaptive immunity, high parasitaemia was observed in the RAG1 knockout mice. The food limited mice (malnourished) had reduced numbers of CD4+ T cells, TNF proportions, and significantly greater Tbet expression compared to the control group. Supplementation with Moringa in the limited group slightly restored ... |
format |
Article in Journal/Newspaper |
author |
Jennifer Pilotos Kadra Abdu Ibrahim Chishimba Nathan Mowa Michael Makokha Opata |
author_facet |
Jennifer Pilotos Kadra Abdu Ibrahim Chishimba Nathan Mowa Michael Makokha Opata |
author_sort |
Jennifer Pilotos |
title |
Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice |
title_short |
Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice |
title_full |
Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice |
title_fullStr |
Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice |
title_full_unstemmed |
Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice |
title_sort |
moringa oleifera treatment increases tbet expression in cd4+ t cells and remediates immune defects of malnutrition in plasmodium chabaudi-infected mice |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-3129-8 https://doaj.org/article/4cae3cd7a49143c9be2bf46e5e6700c8 |
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Arctic |
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Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-16 (2020) |
op_relation |
https://doi.org/10.1186/s12936-020-3129-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-3129-8 1475-2875 https://doaj.org/article/4cae3cd7a49143c9be2bf46e5e6700c8 |
op_doi |
https://doi.org/10.1186/s12936-020-3129-8 |
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Malaria Journal |
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19 |
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1766347605620555776 |