Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus
Abstract Background: Amphibians inhabit the terrestrial environment, a conquest achieved after several evolutionary steps, which were still insufficient to make them completely independent of the aquatic environment. These processes gave rise to many morphological and physiological changes, making t...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2020-0105 https://doaj.org/article/4cacad0817854665a7204de3d743b9bb |
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ftdoajarticles:oai:doaj.org/article:4cacad0817854665a7204de3d743b9bb 2023-05-15T15:12:47+02:00 Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus Douglas O. Mariano Juliana M. Sciani Marta M. Antoniazzi Carlos Jared Katia Conceição Daniel C. Pimenta 2021-04-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2020-0105 https://doaj.org/article/4cacad0817854665a7204de3d743b9bb EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100306&tlng=en http://www.scielo.br/pdf/jvatitd/v27/1678-9199-jvatitd-27-e20200105.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0105 https://doaj.org/article/4cacad0817854665a7204de3d743b9bb Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) Peptides Peptidomics Proteins Proteomics Pithecopus nordestinus Tree frog Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2021 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2020-0105 2022-12-31T16:06:25Z Abstract Background: Amphibians inhabit the terrestrial environment, a conquest achieved after several evolutionary steps, which were still insufficient to make them completely independent of the aquatic environment. These processes gave rise to many morphological and physiological changes, making their skin (and cutaneous secretion) rich in bioactive molecules. Among the tree frogs, the secretion is composed mainly of peptides; but alkaloids, proteins and steroids can also be found depending on the species. The most known class of biologically active molecules is the antimicrobial peptides (AMPs) that act against bacteria, fungi and protozoans. Although these molecules are well-studied among the hylids, AMPs ontogeny remains unknown. Therefore, we performed peptidomic and proteomic analyses of Pithecopus nordestinus (formerly Phyllomedusa nordestina) in order to evaluate the peptide content in post-metamorphosed juveniles and adult individuals. Methods: Cutaneous secretion of both life stages of individuals was obtained and analyzed by LC-MS/MS after reduction and alkylation of disulfide bonds or reduction, alkylation and hydrolysis by trypsin. Results: Differences in the TIC profile of juveniles and adults in both treatments were observed. Moreover, the proteomic data revealed known proteins and peptides, with slight differences in the composition, according to the life stage and the treatment. AMPs were identified, and bradykinin-potentiating peptides were observed in trypsin-treated samples, which suggests a protein source of such peptide (cryptide). Conclusion: In general, skin secretion contents were similar between juveniles and adults, varying in quantity, indicating that the different stages of life are reflected in the number of molecules and not on their diversity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 27 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Peptides Peptidomics Proteins Proteomics Pithecopus nordestinus Tree frog Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Peptides Peptidomics Proteins Proteomics Pithecopus nordestinus Tree frog Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Douglas O. Mariano Juliana M. Sciani Marta M. Antoniazzi Carlos Jared Katia Conceição Daniel C. Pimenta Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus |
topic_facet |
Peptides Peptidomics Proteins Proteomics Pithecopus nordestinus Tree frog Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Amphibians inhabit the terrestrial environment, a conquest achieved after several evolutionary steps, which were still insufficient to make them completely independent of the aquatic environment. These processes gave rise to many morphological and physiological changes, making their skin (and cutaneous secretion) rich in bioactive molecules. Among the tree frogs, the secretion is composed mainly of peptides; but alkaloids, proteins and steroids can also be found depending on the species. The most known class of biologically active molecules is the antimicrobial peptides (AMPs) that act against bacteria, fungi and protozoans. Although these molecules are well-studied among the hylids, AMPs ontogeny remains unknown. Therefore, we performed peptidomic and proteomic analyses of Pithecopus nordestinus (formerly Phyllomedusa nordestina) in order to evaluate the peptide content in post-metamorphosed juveniles and adult individuals. Methods: Cutaneous secretion of both life stages of individuals was obtained and analyzed by LC-MS/MS after reduction and alkylation of disulfide bonds or reduction, alkylation and hydrolysis by trypsin. Results: Differences in the TIC profile of juveniles and adults in both treatments were observed. Moreover, the proteomic data revealed known proteins and peptides, with slight differences in the composition, according to the life stage and the treatment. AMPs were identified, and bradykinin-potentiating peptides were observed in trypsin-treated samples, which suggests a protein source of such peptide (cryptide). Conclusion: In general, skin secretion contents were similar between juveniles and adults, varying in quantity, indicating that the different stages of life are reflected in the number of molecules and not on their diversity. |
format |
Article in Journal/Newspaper |
author |
Douglas O. Mariano Juliana M. Sciani Marta M. Antoniazzi Carlos Jared Katia Conceição Daniel C. Pimenta |
author_facet |
Douglas O. Mariano Juliana M. Sciani Marta M. Antoniazzi Carlos Jared Katia Conceição Daniel C. Pimenta |
author_sort |
Douglas O. Mariano |
title |
Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus |
title_short |
Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus |
title_full |
Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus |
title_fullStr |
Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus |
title_full_unstemmed |
Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus |
title_sort |
quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog pithecopus nordestinus |
publisher |
SciELO |
publishDate |
2021 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0105 https://doaj.org/article/4cacad0817854665a7204de3d743b9bb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100306&tlng=en http://www.scielo.br/pdf/jvatitd/v27/1678-9199-jvatitd-27-e20200105.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0105 https://doaj.org/article/4cacad0817854665a7204de3d743b9bb |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0105 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
27 |
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1766343426459041792 |