Environmental straits of Cryptococcus neoformans variety grubii in the city of Santos, SP, Brazil Amostras ambientais de Cryptococcus neoformans var. grubii na cidade de Santos, SP, Brasil

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Maria Cecília Bianchi Soares, Claudete Rodrigues Paula, Amanda L.T. Dias, Marcos Montani Caseiro, Sérgio Olavo Pinto da Costa
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2005
• Subjects: Cryptococcus neoformans; Pigeon droppings; Serotype; Antifungal agents; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic; Elevado
• Online Access: https://doi.org/10.1590/S0036-46652005000100006; https://doaj.org/article/4ca898894653495faa893bdd39c28bff

This study involved a total of 116 samples, 79 taken from pigeon droppings and 37 of atmospheric air taken close to accumulations of excrement. Cryptococcus neoformans var. grubii was isolated from 11 (13.9%) of these samples. Other species of Cryptococcus were also isolated from these samples, such as C. albidus (12.6%) and C. laurentii (8.9%). C. neoformans was not isolated from the air samples, though C. albidus (5.4%) was. All the strains of C. neoformans were found to belong to the A serotype (C. neoformans var. grubii). In regard to the studies with the antifungal agents 5-fluorocytosine, fluconazole, itraconazole, amphotericin B and voriconazole, by means of the microdilution method (EUCAST), we point out that one sample demonstrated resistance to fluconazole, this being especially significant because this is an environmental strain. Analisaram-se 116 amostras, sendo 79 de fezes de pombos e 37 de ar atmosférico de regiões com acúmulo de fezes. Isolou-se Cryptococcus neoformans var. neoformans de 11 (13.9%) destas amostras. Outras espécies de Cryptococcus também foram isoladas destas amostras tais como C. laurentii (8.9%) e C. albidus (12.6 %), o qual também foi isolado de amostras do ar (5.4 %). Todas as amostras de C. neoformans foram sorotipo A (C. neoformans var. grubii). Em relação à avaliação do perfil de sensibilidade às drogas antifúngicas (5-fluorocitosina, fluconazol, itraconazol, anfotericina B e voriconazol) pelo método da microdiluição (EUCAST, 2002), destacou-se a presença de uma amostra com valor de concentração inibitória mínima (CIM) elevado para fluconazol, sendo de grande significância, uma vez tratar-se de isolado ambiental.