Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities
ABSTRACT Microbial activity in Arctic soils controls the cycling of significant stores of organic carbon and nutrients. We studied in situ processes in Alaskan soils using original metaproteomic methods in order to relate important heterotrophic functions to microbial taxa and to understand the micr...
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American Society for Microbiology
2023
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ftdoajarticles:oai:doaj.org/article:4c8b698f6cf94a5aa247645e88f15178 2023-07-23T04:16:48+02:00 Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities Samuel E. Miller Albert S. Colman Jacob R. Waldbauer 2023-06-01T00:00:00Z https://doi.org/10.1128/msystems.01238-22 https://doaj.org/article/4c8b698f6cf94a5aa247645e88f15178 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/msystems.01238-22 https://doaj.org/toc/2379-5077 doi:10.1128/msystems.01238-22 2379-5077 https://doaj.org/article/4c8b698f6cf94a5aa247645e88f15178 mSystems, Vol 8, Iss 3 (2023) soil arctic metaproteomics permafrost metabolism microbial ecology Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.1128/msystems.01238-22 2023-07-02T00:35:10Z ABSTRACT Microbial activity in Arctic soils controls the cycling of significant stores of organic carbon and nutrients. We studied in situ processes in Alaskan soils using original metaproteomic methods in order to relate important heterotrophic functions to microbial taxa and to understand the microbial response to Arctic greening. Major bacterial groups show strong metabolic specialization in organic topsoils. α-/β-/γ-Proteobacteria specialized in the acquisition of small, soluble compounds, whereas Acidobacteria, Actinobacteria, and other detritosphere groups specialized in the degradation of plant-derived polymers. α-/β-/γ-Proteobacteria dominated the expression of transporters for common root exudates and limiting nitrogenous compounds, supporting an ecological model of dependence upon plants for carbon and competition with plants for nitrogen. Detritosphere groups specialized in distinct substrates, with Acidobacteria producing the most enzymes for hemicellulose depolymerization. Acidobacteria was the most active group across the three plant ecotypes sampled—the largely nonvascular, lower biomass intertussock and the largely vascular, higher biomass tussock and shrub. Functional partitioning among bacterial groups was stable between plant ecotypes, but certain functions associated with α-/β-/γ-Proteobacteria were more strongly expressed in higher biomass ecotypes. We show that refined metaproteomic approaches can elucidate soil microbial ecology as well as biogeochemical trajectories of major carbon stocks. IMPORTANCE The Arctic is warming twice as fast as the rest of the planet, and Arctic soils currently store twice as much carbon as the entire atmosphere—two facts that make understanding how Arctic soil microbial communities are responding to climate change particularly urgent. Greening of vegetation cover across the Arctic landscape is one of the most prominent climate-driven shifts in Arctic terrestrial ecology, with potentially profound effects on biogeochemical cycling by the soil microbiome. Here ... Article in Journal/Newspaper Arctic Greening Arctic Climate change permafrost Directory of Open Access Journals: DOAJ Articles Arctic mSystems 8 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
soil arctic metaproteomics permafrost metabolism microbial ecology Microbiology QR1-502 |
spellingShingle |
soil arctic metaproteomics permafrost metabolism microbial ecology Microbiology QR1-502 Samuel E. Miller Albert S. Colman Jacob R. Waldbauer Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
topic_facet |
soil arctic metaproteomics permafrost metabolism microbial ecology Microbiology QR1-502 |
description |
ABSTRACT Microbial activity in Arctic soils controls the cycling of significant stores of organic carbon and nutrients. We studied in situ processes in Alaskan soils using original metaproteomic methods in order to relate important heterotrophic functions to microbial taxa and to understand the microbial response to Arctic greening. Major bacterial groups show strong metabolic specialization in organic topsoils. α-/β-/γ-Proteobacteria specialized in the acquisition of small, soluble compounds, whereas Acidobacteria, Actinobacteria, and other detritosphere groups specialized in the degradation of plant-derived polymers. α-/β-/γ-Proteobacteria dominated the expression of transporters for common root exudates and limiting nitrogenous compounds, supporting an ecological model of dependence upon plants for carbon and competition with plants for nitrogen. Detritosphere groups specialized in distinct substrates, with Acidobacteria producing the most enzymes for hemicellulose depolymerization. Acidobacteria was the most active group across the three plant ecotypes sampled—the largely nonvascular, lower biomass intertussock and the largely vascular, higher biomass tussock and shrub. Functional partitioning among bacterial groups was stable between plant ecotypes, but certain functions associated with α-/β-/γ-Proteobacteria were more strongly expressed in higher biomass ecotypes. We show that refined metaproteomic approaches can elucidate soil microbial ecology as well as biogeochemical trajectories of major carbon stocks. IMPORTANCE The Arctic is warming twice as fast as the rest of the planet, and Arctic soils currently store twice as much carbon as the entire atmosphere—two facts that make understanding how Arctic soil microbial communities are responding to climate change particularly urgent. Greening of vegetation cover across the Arctic landscape is one of the most prominent climate-driven shifts in Arctic terrestrial ecology, with potentially profound effects on biogeochemical cycling by the soil microbiome. Here ... |
format |
Article in Journal/Newspaper |
author |
Samuel E. Miller Albert S. Colman Jacob R. Waldbauer |
author_facet |
Samuel E. Miller Albert S. Colman Jacob R. Waldbauer |
author_sort |
Samuel E. Miller |
title |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_short |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_full |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_fullStr |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_full_unstemmed |
Metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected Arctic soil bacterial communities |
title_sort |
metaproteomics reveals functional partitioning and vegetational variation among permafrost-affected arctic soil bacterial communities |
publisher |
American Society for Microbiology |
publishDate |
2023 |
url |
https://doi.org/10.1128/msystems.01238-22 https://doaj.org/article/4c8b698f6cf94a5aa247645e88f15178 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Greening Arctic Climate change permafrost |
genre_facet |
Arctic Greening Arctic Climate change permafrost |
op_source |
mSystems, Vol 8, Iss 3 (2023) |
op_relation |
https://journals.asm.org/doi/10.1128/msystems.01238-22 https://doaj.org/toc/2379-5077 doi:10.1128/msystems.01238-22 2379-5077 https://doaj.org/article/4c8b698f6cf94a5aa247645e88f15178 |
op_doi |
https://doi.org/10.1128/msystems.01238-22 |
container_title |
mSystems |
container_volume |
8 |
container_issue |
3 |
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1772177919287754752 |