Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru
Abstract Background Erythrocyte invasion by Plasmodium falciparum is a complex process that involves two families; Erythrocyte Binding-Like (EBL) and the Reticulocyte Binding-Like (PfRh) proteins. Antibodies that inhibit merozoite attachment and invasion are believed to be important in mediating nat...
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ftdoajarticles:oai:doaj.org/article:4c7e24866bc14adaa08a0d36797f8def 2023-05-15T15:15:49+02:00 Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru Villasis Elizabeth Lopez-Perez Mary Torres Katherine Gamboa Dionicia Neyra Victor Bendezu Jorge Tricoche Nancy Lobo Cheryl Vinetz Joseph M Lustigman Sara 2012-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-361 https://doaj.org/article/4c7e24866bc14adaa08a0d36797f8def EN eng BMC http://www.malariajournal.com/content/11/1/361 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-361 1475-2875 https://doaj.org/article/4c7e24866bc14adaa08a0d36797f8def Malaria Journal, Vol 11, Iss 1, p 361 (2012) Antibodies Invasion Plasmodium falciparum Malaria Peru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-361 2022-12-31T09:32:22Z Abstract Background Erythrocyte invasion by Plasmodium falciparum is a complex process that involves two families; Erythrocyte Binding-Like (EBL) and the Reticulocyte Binding-Like (PfRh) proteins. Antibodies that inhibit merozoite attachment and invasion are believed to be important in mediating naturally acquired immunity and immunity generated by parasite blood stage vaccine candidates. The hypotheses tested in this study were 1) that antibody responses against specific P. falciparum invasion ligands (EBL and PfRh) differ between symptomatic and asymptomatic individuals living in the low-transmission region of the Peruvian Amazon and 2), such antibody responses might have an association, either direct or indirect, with clinical immunity observed in asymptomatically parasitaemic individuals. Methods ELISA was used to assess antibody responses (IgG, IgG1 and IgG3) against recombinant P. falciparum invasion ligands of the EBL (EBA-175, EBA-181, EBA-140) and PfRh families (PfRh1, PfRh2a, PfRh2b, PfRh4 and PfRh5) in 45 individuals infected with P. falciparum from Peruvian Amazon. Individuals were classified as having symptomatic malaria (N=37) or asymptomatic infection (N=8). Results Antibody responses against both EBL and PfRh family proteins were significantly higher in asymptomatic compared to symptomatic individuals, demonstrating an association with clinical immunity. Significant differences in the total IgG responses were observed with EBA-175, EBA-181, PfRh2b, and MSP1 19 (as a control). IgG1 responses against EBA-181, PfRh2a and PfRh2b were significantly higher in the asymptomatic individuals. Total IgG antibody responses against PfRh1, PfRh2a, PfRh2b, PfRh5, EBA-175, EBA-181 and MSP1 19 proteins were negatively correlated with level of parasitaemia. IgG1 responses against EBA-181, PfRh2a and PfRh2b and IgG3 response for PfRh2a were also negatively correlated with parasitaemia. Conclusions These data suggest that falciparum malaria patients who develop clinical immunity (asymptomatic parasitaemia) in a low ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 361 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Antibodies Invasion Plasmodium falciparum Malaria Peru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Antibodies Invasion Plasmodium falciparum Malaria Peru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Villasis Elizabeth Lopez-Perez Mary Torres Katherine Gamboa Dionicia Neyra Victor Bendezu Jorge Tricoche Nancy Lobo Cheryl Vinetz Joseph M Lustigman Sara Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru |
topic_facet |
Antibodies Invasion Plasmodium falciparum Malaria Peru Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Erythrocyte invasion by Plasmodium falciparum is a complex process that involves two families; Erythrocyte Binding-Like (EBL) and the Reticulocyte Binding-Like (PfRh) proteins. Antibodies that inhibit merozoite attachment and invasion are believed to be important in mediating naturally acquired immunity and immunity generated by parasite blood stage vaccine candidates. The hypotheses tested in this study were 1) that antibody responses against specific P. falciparum invasion ligands (EBL and PfRh) differ between symptomatic and asymptomatic individuals living in the low-transmission region of the Peruvian Amazon and 2), such antibody responses might have an association, either direct or indirect, with clinical immunity observed in asymptomatically parasitaemic individuals. Methods ELISA was used to assess antibody responses (IgG, IgG1 and IgG3) against recombinant P. falciparum invasion ligands of the EBL (EBA-175, EBA-181, EBA-140) and PfRh families (PfRh1, PfRh2a, PfRh2b, PfRh4 and PfRh5) in 45 individuals infected with P. falciparum from Peruvian Amazon. Individuals were classified as having symptomatic malaria (N=37) or asymptomatic infection (N=8). Results Antibody responses against both EBL and PfRh family proteins were significantly higher in asymptomatic compared to symptomatic individuals, demonstrating an association with clinical immunity. Significant differences in the total IgG responses were observed with EBA-175, EBA-181, PfRh2b, and MSP1 19 (as a control). IgG1 responses against EBA-181, PfRh2a and PfRh2b were significantly higher in the asymptomatic individuals. Total IgG antibody responses against PfRh1, PfRh2a, PfRh2b, PfRh5, EBA-175, EBA-181 and MSP1 19 proteins were negatively correlated with level of parasitaemia. IgG1 responses against EBA-181, PfRh2a and PfRh2b and IgG3 response for PfRh2a were also negatively correlated with parasitaemia. Conclusions These data suggest that falciparum malaria patients who develop clinical immunity (asymptomatic parasitaemia) in a low ... |
format |
Article in Journal/Newspaper |
author |
Villasis Elizabeth Lopez-Perez Mary Torres Katherine Gamboa Dionicia Neyra Victor Bendezu Jorge Tricoche Nancy Lobo Cheryl Vinetz Joseph M Lustigman Sara |
author_facet |
Villasis Elizabeth Lopez-Perez Mary Torres Katherine Gamboa Dionicia Neyra Victor Bendezu Jorge Tricoche Nancy Lobo Cheryl Vinetz Joseph M Lustigman Sara |
author_sort |
Villasis Elizabeth |
title |
Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru |
title_short |
Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru |
title_full |
Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru |
title_fullStr |
Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru |
title_full_unstemmed |
Anti- Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru |
title_sort |
anti- plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, loreto, peru |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-361 https://doaj.org/article/4c7e24866bc14adaa08a0d36797f8def |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 361 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/361 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-361 1475-2875 https://doaj.org/article/4c7e24866bc14adaa08a0d36797f8def |
op_doi |
https://doi.org/10.1186/1475-2875-11-361 |
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Malaria Journal |
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11 |
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361 |
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1766346154393468928 |