HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements
High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is o...
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MDPI AG
2021
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Online Access: | https://doi.org/10.3390/w13213030 https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f |
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ftdoajarticles:oai:doaj.org/article:4c47ff8a1caa4b30bfab358be052389f 2023-05-15T13:46:37+02:00 HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni 2021-10-01T00:00:00Z https://doi.org/10.3390/w13213030 https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f EN eng MDPI AG https://www.mdpi.com/2073-4441/13/21/3030 https://doaj.org/toc/2073-4441 doi:10.3390/w13213030 2073-4441 https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f Water, Vol 13, Iss 3030, p 3030 (2021) pH meta-cresol purple HPLC ocean acidification Ross Sea Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 article 2021 ftdoajarticles https://doi.org/10.3390/w13213030 2022-12-31T05:06:29Z High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and therefore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH 3 CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface. Article in Journal/Newspaper Antarc* Antarctica Ocean acidification Ross Sea Directory of Open Access Journals: DOAJ Articles Ross Sea Water 13 21 3030 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
pH meta-cresol purple HPLC ocean acidification Ross Sea Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 |
spellingShingle |
pH meta-cresol purple HPLC ocean acidification Ross Sea Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
topic_facet |
pH meta-cresol purple HPLC ocean acidification Ross Sea Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 |
description |
High-quality pH measurements are essential for observing ocean acidification and interpreting its chemical and ecological effects. Spectrophotometric measure is the preferred method for pH analysis of seawater because of its high reproducibility and excellent precision. Meta-cresol purple (mCP) is one of the most used indicator dyes for spectrophotometric pH determination. Impurities in indicator salts can significantly bias spectrophotometric pH determinations, and therefore the use of purified mCP is recommended. This work describes the use of a fast, simple, and green-friendly High-Performance-Liquid Chromatography (HPLC) procedure for purification of mCP dye solution. A C18 reversed phase column was used, and different compositions of the mobile phase were considered. The selected mobile phase was 85% Milli-Q water and 15% CH 3 CN pH 8.0 that allowed reasonable run time and a suitable purified mCP retention time (4.65 ± 0.005 min). Aliquots of purified mCP were mixed and evaporated by rotary evaporator that resulted in the fastest preconcentration procedure among those considered in this study. Purified mCP was used for measuring the pH in seawater samples collected in the Ross Sea (Antarctica) and the values ranged between 8.202 and 7.983, with the highest values at the surface. |
format |
Article in Journal/Newspaper |
author |
Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni |
author_facet |
Paola Rivaro Davide Vivado Pierpaolo Falco Carmela Ianni |
author_sort |
Paola Rivaro |
title |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_short |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_full |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_fullStr |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_full_unstemmed |
HPLC-DAD Purification and Characterization of Meta-Cresol-Purple for Spectrophotometric Seawater pH Measurements |
title_sort |
hplc-dad purification and characterization of meta-cresol-purple for spectrophotometric seawater ph measurements |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/w13213030 https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ocean acidification Ross Sea |
genre_facet |
Antarc* Antarctica Ocean acidification Ross Sea |
op_source |
Water, Vol 13, Iss 3030, p 3030 (2021) |
op_relation |
https://www.mdpi.com/2073-4441/13/21/3030 https://doaj.org/toc/2073-4441 doi:10.3390/w13213030 2073-4441 https://doaj.org/article/4c47ff8a1caa4b30bfab358be052389f |
op_doi |
https://doi.org/10.3390/w13213030 |
container_title |
Water |
container_volume |
13 |
container_issue |
21 |
container_start_page |
3030 |
_version_ |
1766244706344239104 |