Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis.
Mast cells are innate effector cells that due to their localization in the tissue form the first line of defense against parasites. We have previously shown that specifically mucosal mast cells were essential for the termination of the intestinal Strongyloides ratti infection. Here, we analyze the i...
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ftdoajarticles:oai:doaj.org/article:4c01a216f5f94c8da75097922d414f65 2023-05-15T15:09:57+02:00 Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis. Lara Christine Linnemann Martina Reitz Thorsten B Feyerabend Minka Breloer Wiebke Hartmann 2020-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008534 https://doaj.org/article/4c01a216f5f94c8da75097922d414f65 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008534 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008534 https://doaj.org/article/4c01a216f5f94c8da75097922d414f65 PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008534 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008534 2022-12-31T09:15:21Z Mast cells are innate effector cells that due to their localization in the tissue form the first line of defense against parasites. We have previously shown that specifically mucosal mast cells were essential for the termination of the intestinal Strongyloides ratti infection. Here, we analyze the impact of mast cells on the immune response and defense against the tissue-dwelling filarial nematode Litomosoides sigmodontis using mast cell-deficient Cpa3cre mice. Despite an increase and an activation of mast cells at the site of infection in wildtype BALB/c mice the outcome of L. sigmodontis infection was not changed in mast cell-deficient BALB/c Cpa3cre mice. In Cpa3cre mice neither vascular permeability induced by blood-sucking mites nor the migration of L3 was altered compared to Cpa3 wildtype littermates. Worm burden in the thoracic cavity was alike in the presence and absence of mast cells during the entire course of infection. Although microfilaremiae in the peripheral blood increased in mast cell-deficient mice at some time points, the infection was cleared with comparable kinetics in the presence and absence of mast cells. Moreover, mast cell deficiency had no impact on the cytokine and antibody response to L. sigmodontis. In summary, our findings suggest that mast cells are not mandatory for the initiation of an appropriate immune response and host defense during L. sigmodontis infection in mice. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 7 e0008534 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lara Christine Linnemann Martina Reitz Thorsten B Feyerabend Minka Breloer Wiebke Hartmann Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mast cells are innate effector cells that due to their localization in the tissue form the first line of defense against parasites. We have previously shown that specifically mucosal mast cells were essential for the termination of the intestinal Strongyloides ratti infection. Here, we analyze the impact of mast cells on the immune response and defense against the tissue-dwelling filarial nematode Litomosoides sigmodontis using mast cell-deficient Cpa3cre mice. Despite an increase and an activation of mast cells at the site of infection in wildtype BALB/c mice the outcome of L. sigmodontis infection was not changed in mast cell-deficient BALB/c Cpa3cre mice. In Cpa3cre mice neither vascular permeability induced by blood-sucking mites nor the migration of L3 was altered compared to Cpa3 wildtype littermates. Worm burden in the thoracic cavity was alike in the presence and absence of mast cells during the entire course of infection. Although microfilaremiae in the peripheral blood increased in mast cell-deficient mice at some time points, the infection was cleared with comparable kinetics in the presence and absence of mast cells. Moreover, mast cell deficiency had no impact on the cytokine and antibody response to L. sigmodontis. In summary, our findings suggest that mast cells are not mandatory for the initiation of an appropriate immune response and host defense during L. sigmodontis infection in mice. |
format |
Article in Journal/Newspaper |
author |
Lara Christine Linnemann Martina Reitz Thorsten B Feyerabend Minka Breloer Wiebke Hartmann |
author_facet |
Lara Christine Linnemann Martina Reitz Thorsten B Feyerabend Minka Breloer Wiebke Hartmann |
author_sort |
Lara Christine Linnemann |
title |
Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis. |
title_short |
Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis. |
title_full |
Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis. |
title_fullStr |
Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis. |
title_full_unstemmed |
Limited role of mast cells during infection with the parasitic nematode Litomosoides sigmodontis. |
title_sort |
limited role of mast cells during infection with the parasitic nematode litomosoides sigmodontis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008534 https://doaj.org/article/4c01a216f5f94c8da75097922d414f65 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008534 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008534 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008534 https://doaj.org/article/4c01a216f5f94c8da75097922d414f65 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008534 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
7 |
container_start_page |
e0008534 |
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1766341046483025920 |