Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known a...
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ftdoajarticles:oai:doaj.org/article:4bf60445a5b04e1e8c5d7db0990ac4e5 2024-01-07T09:45:03+01:00 Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe Peter Shum Lauren Moore Christophe Pampoulie Cristina Di Muri Sara Vandamme Stefano Mariani 2017-10-01T00:00:00Z https://doi.org/10.7717/peerj.3746 https://doaj.org/article/4bf60445a5b04e1e8c5d7db0990ac4e5 EN eng PeerJ Inc. https://peerj.com/articles/3746.pdf https://peerj.com/articles/3746/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.3746 2167-8359 https://doaj.org/article/4bf60445a5b04e1e8c5d7db0990ac4e5 PeerJ, Vol 5, p e3746 (2017) Sebastes Redfish Barcoding Mislabelling Seafood COI Medicine R Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.7717/peerj.3746 2023-12-10T01:49:45Z Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Pacific PeerJ 5 e3746 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Sebastes Redfish Barcoding Mislabelling Seafood COI Medicine R Biology (General) QH301-705.5 |
spellingShingle |
Sebastes Redfish Barcoding Mislabelling Seafood COI Medicine R Biology (General) QH301-705.5 Peter Shum Lauren Moore Christophe Pampoulie Cristina Di Muri Sara Vandamme Stefano Mariani Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
topic_facet |
Sebastes Redfish Barcoding Mislabelling Seafood COI Medicine R Biology (General) QH301-705.5 |
description |
Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples. |
format |
Article in Journal/Newspaper |
author |
Peter Shum Lauren Moore Christophe Pampoulie Cristina Di Muri Sara Vandamme Stefano Mariani |
author_facet |
Peter Shum Lauren Moore Christophe Pampoulie Cristina Di Muri Sara Vandamme Stefano Mariani |
author_sort |
Peter Shum |
title |
Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_short |
Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_full |
Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_fullStr |
Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_full_unstemmed |
Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_sort |
harnessing mtdna variation to resolve ambiguity in ‘redfish’ sold in europe |
publisher |
PeerJ Inc. |
publishDate |
2017 |
url |
https://doi.org/10.7717/peerj.3746 https://doaj.org/article/4bf60445a5b04e1e8c5d7db0990ac4e5 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
PeerJ, Vol 5, p e3746 (2017) |
op_relation |
https://peerj.com/articles/3746.pdf https://peerj.com/articles/3746/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.3746 2167-8359 https://doaj.org/article/4bf60445a5b04e1e8c5d7db0990ac4e5 |
op_doi |
https://doi.org/10.7717/peerj.3746 |
container_title |
PeerJ |
container_volume |
5 |
container_start_page |
e3746 |
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1787426499929833472 |