Assessing Serratia spp. pathogenic potential from cryogenic habitats
The genus Serratia are opportunistic bacteria widely spread in natural environment. At the same time, this bacterial genus consists of the species associated with outbreaks of nosocomial infections. Serratia species are found in extreme habitats, but pathogenic potential of polyextremophilic strains...
Published in: | Russian Journal of Infection and Immunity |
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Language: | Russian |
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Sankt-Peterburg : NIIÈM imeni Pastera
2021
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Online Access: | https://doi.org/10.15789/2220-7619-ASS-1593 https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced6 |
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ftdoajarticles:oai:doaj.org/article:4bcccb4db5c54f24b1e2fad6f8abced6 2023-05-15T13:04:55+02:00 Assessing Serratia spp. pathogenic potential from cryogenic habitats A. E. Goncharov A. P. Solomenny A. L. Panin S. E. Grigoriev M. Yu. Cheprasov Ya. A. Ahremenko V. V. Kolodzieva N. E. Goncharov L. A. Kraeva 2021-06-01T00:00:00Z https://doi.org/10.15789/2220-7619-ASS-1593 https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced6 RU rus Sankt-Peterburg : NIIÈM imeni Pastera https://www.iimmun.ru/iimm/article/view/1593 https://doaj.org/toc/2220-7619 https://doaj.org/toc/2313-7398 2220-7619 2313-7398 doi:10.15789/2220-7619-ASS-1593 https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced6 Infekciâ i Immunitet, Vol 11, Iss 3, Pp 585-590 (2021) serratia biofilms extreme environment psychrotolerant bacteria cold adaptation virulence microbiological monitoring Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.15789/2220-7619-ASS-1593 2022-12-31T12:57:32Z The genus Serratia are opportunistic bacteria widely spread in natural environment. At the same time, this bacterial genus consists of the species associated with outbreaks of nosocomial infections. Serratia species are found in extreme habitats, but pathogenic potential of polyextremophilic strains in this genus remains unexplored. The aim of this study was to compare the genomes of two Serratia strains isolated in polar regions, primarily examining genetic factors of virulence and adaptation to cryogenic environment. During the 56th Russian Antarctic Expedition the Serratia liquefaciens 72 strain was isolated from a guano sample of the Adelie Penguin (Pygoscelis adeliae) colony on Tokarev Island (Haswell Archipelago, East Antarctica). The Serratia fonticola 5l strain was isolated from the frozen carcass of moose (Alces alces) fossils found on the Buor-Khaya Peninsula near the Laptev Sea coast (Yakutia Region, Russia). The whole-genome sequencing of such strains allowed to reveal genetic structures evidencing about their successful adaptation to low temperatures. Thus, it was found that both genomes contain genes encoding the main cold shock proteins, phylogenetically close to the corresponding genes in the hypobarotolerant Serratia liquefaciens strain ATCC 27592. Furthermore, both strains bear a cluster of tc-fABCD genes determining the bacterial adhesion to epithelial tissues, and the genes for RTX toxins — adhesins, crucial factors of biofilm formation in pathogenic Gram-negative bacteria. Experimental studies confirmed the ability of Serratia liquefaciens 72 and Serratia fonticola 5l to actively form biofilms in a wide temperature range (from 6°C to 37°C). The results obtained indicate that the examined genus Serratia strains isolated in Arctica and Antarctica exert overall similar adaptation strategies to polar climate, including the ability to produce pili, show active adhesion, and biofilm formation under low temperatures. Genetic adaptive factors may also act as pathogenicity factors allowing ... Article in Journal/Newspaper Adelie penguin Alces alces Antarc* Antarctic Antarctica East Antarctica laptev Laptev Sea Pygoscelis adeliae Tokarev Island Yakutia Directory of Open Access Journals: DOAJ Articles Antarctic East Antarctica Laptev Sea Guano ENVELOPE(141.604,141.604,-66.775,-66.775) Khaya ENVELOPE(135.167,135.167,60.567,60.567) Buor-Khaya ENVELOPE(127.803,127.803,72.287,72.287) Tokarev ENVELOPE(152.433,152.433,-68.467,-68.467) Tokarev Island ENVELOPE(92.983,92.983,-66.533,-66.533) Russian Journal of Infection and Immunity 11 3 585 590 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
Russian |
topic |
serratia biofilms extreme environment psychrotolerant bacteria cold adaptation virulence microbiological monitoring Infectious and parasitic diseases RC109-216 |
spellingShingle |
serratia biofilms extreme environment psychrotolerant bacteria cold adaptation virulence microbiological monitoring Infectious and parasitic diseases RC109-216 A. E. Goncharov A. P. Solomenny A. L. Panin S. E. Grigoriev M. Yu. Cheprasov Ya. A. Ahremenko V. V. Kolodzieva N. E. Goncharov L. A. Kraeva Assessing Serratia spp. pathogenic potential from cryogenic habitats |
topic_facet |
serratia biofilms extreme environment psychrotolerant bacteria cold adaptation virulence microbiological monitoring Infectious and parasitic diseases RC109-216 |
description |
The genus Serratia are opportunistic bacteria widely spread in natural environment. At the same time, this bacterial genus consists of the species associated with outbreaks of nosocomial infections. Serratia species are found in extreme habitats, but pathogenic potential of polyextremophilic strains in this genus remains unexplored. The aim of this study was to compare the genomes of two Serratia strains isolated in polar regions, primarily examining genetic factors of virulence and adaptation to cryogenic environment. During the 56th Russian Antarctic Expedition the Serratia liquefaciens 72 strain was isolated from a guano sample of the Adelie Penguin (Pygoscelis adeliae) colony on Tokarev Island (Haswell Archipelago, East Antarctica). The Serratia fonticola 5l strain was isolated from the frozen carcass of moose (Alces alces) fossils found on the Buor-Khaya Peninsula near the Laptev Sea coast (Yakutia Region, Russia). The whole-genome sequencing of such strains allowed to reveal genetic structures evidencing about their successful adaptation to low temperatures. Thus, it was found that both genomes contain genes encoding the main cold shock proteins, phylogenetically close to the corresponding genes in the hypobarotolerant Serratia liquefaciens strain ATCC 27592. Furthermore, both strains bear a cluster of tc-fABCD genes determining the bacterial adhesion to epithelial tissues, and the genes for RTX toxins — adhesins, crucial factors of biofilm formation in pathogenic Gram-negative bacteria. Experimental studies confirmed the ability of Serratia liquefaciens 72 and Serratia fonticola 5l to actively form biofilms in a wide temperature range (from 6°C to 37°C). The results obtained indicate that the examined genus Serratia strains isolated in Arctica and Antarctica exert overall similar adaptation strategies to polar climate, including the ability to produce pili, show active adhesion, and biofilm formation under low temperatures. Genetic adaptive factors may also act as pathogenicity factors allowing ... |
format |
Article in Journal/Newspaper |
author |
A. E. Goncharov A. P. Solomenny A. L. Panin S. E. Grigoriev M. Yu. Cheprasov Ya. A. Ahremenko V. V. Kolodzieva N. E. Goncharov L. A. Kraeva |
author_facet |
A. E. Goncharov A. P. Solomenny A. L. Panin S. E. Grigoriev M. Yu. Cheprasov Ya. A. Ahremenko V. V. Kolodzieva N. E. Goncharov L. A. Kraeva |
author_sort |
A. E. Goncharov |
title |
Assessing Serratia spp. pathogenic potential from cryogenic habitats |
title_short |
Assessing Serratia spp. pathogenic potential from cryogenic habitats |
title_full |
Assessing Serratia spp. pathogenic potential from cryogenic habitats |
title_fullStr |
Assessing Serratia spp. pathogenic potential from cryogenic habitats |
title_full_unstemmed |
Assessing Serratia spp. pathogenic potential from cryogenic habitats |
title_sort |
assessing serratia spp. pathogenic potential from cryogenic habitats |
publisher |
Sankt-Peterburg : NIIÈM imeni Pastera |
publishDate |
2021 |
url |
https://doi.org/10.15789/2220-7619-ASS-1593 https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced6 |
long_lat |
ENVELOPE(141.604,141.604,-66.775,-66.775) ENVELOPE(135.167,135.167,60.567,60.567) ENVELOPE(127.803,127.803,72.287,72.287) ENVELOPE(152.433,152.433,-68.467,-68.467) ENVELOPE(92.983,92.983,-66.533,-66.533) |
geographic |
Antarctic East Antarctica Laptev Sea Guano Khaya Buor-Khaya Tokarev Tokarev Island |
geographic_facet |
Antarctic East Antarctica Laptev Sea Guano Khaya Buor-Khaya Tokarev Tokarev Island |
genre |
Adelie penguin Alces alces Antarc* Antarctic Antarctica East Antarctica laptev Laptev Sea Pygoscelis adeliae Tokarev Island Yakutia |
genre_facet |
Adelie penguin Alces alces Antarc* Antarctic Antarctica East Antarctica laptev Laptev Sea Pygoscelis adeliae Tokarev Island Yakutia |
op_source |
Infekciâ i Immunitet, Vol 11, Iss 3, Pp 585-590 (2021) |
op_relation |
https://www.iimmun.ru/iimm/article/view/1593 https://doaj.org/toc/2220-7619 https://doaj.org/toc/2313-7398 2220-7619 2313-7398 doi:10.15789/2220-7619-ASS-1593 https://doaj.org/article/4bcccb4db5c54f24b1e2fad6f8abced6 |
op_doi |
https://doi.org/10.15789/2220-7619-ASS-1593 |
container_title |
Russian Journal of Infection and Immunity |
container_volume |
11 |
container_issue |
3 |
container_start_page |
585 |
op_container_end_page |
590 |
_version_ |
1766374950047842304 |