Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria
Many Arctic biomes, which are populated with abundant and diverse microbial life, are under threat: climate change and warming temperatures have raised concerns about diversity loss and possible emergence of pathogenic microorganisms. At present, there is little information on the occurrence of Arct...
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ftdoajarticles:oai:doaj.org/article:4b85800203e544bfbeadeb6d9e00b60f 2023-05-15T14:41:26+02:00 Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria Diana C. Mogrovejo Laura Perini Cene Gostinčar Kristina Sepčić Martina Turk Jerneja Ambrožič-Avguštin Florian H. H. Brill Nina Gunde-Cimerman 2020-04-01T00:00:00Z https://doi.org/10.3389/fmicb.2020.00570 https://doaj.org/article/4b85800203e544bfbeadeb6d9e00b60f EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2020.00570/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00570 https://doaj.org/article/4b85800203e544bfbeadeb6d9e00b60f Frontiers in Microbiology, Vol 11 (2020) Arctic extremely cold environment pathogens hemolysis antimicrobial resistance Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3389/fmicb.2020.00570 2022-12-31T05:11:36Z Many Arctic biomes, which are populated with abundant and diverse microbial life, are under threat: climate change and warming temperatures have raised concerns about diversity loss and possible emergence of pathogenic microorganisms. At present, there is little information on the occurrence of Arctic virulence-associated phenotypes. In this study we worked with 118 strains of bacteria (from 10 sampling sites in the Arctic region, located in Greenland and the Svalbard Archipelago) isolated using R2A medium. These strains belong to 4 phyla and represent 36 different bacterial genera. Phenotypic resistance to 8 clinically important antimicrobials (ampicillin, chloramphenicol, ciprofloxacin, cefotaxime, erythromycin, imipenem, kanamycin, and tetracycline) and thermotolerance range were determined. In addition, a screening of all isolates on blood agar media and erythrocytes suspension of bovine and sheep erythrocytes for virulence-linked hemolytic activity was performed. Although antimicrobial resistance profiles varied among the isolates, they were consistent within bacterial families and genera. Interestingly, a high number of isolates (83/104) were resistant to the tested concentration of imipenem (4 mg/L). In addition, one third of the isolates showed hemolytic activity on blood agar, however, in only 5% of the isolates hemolytic activity was also observed in the cell extracts when added to erythrocyte suspensions for 60 min. The observed microbial phenotypes contribute to our understanding of the presence of virulence-associated factors in the Arctic environments, while highlighting the potential risks associated with changes in the polar areas in the light of climate change. Article in Journal/Newspaper Arctic Climate change Greenland Svalbard Directory of Open Access Journals: DOAJ Articles Arctic Svalbard Svalbard Archipelago Greenland Frontiers in Microbiology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic extremely cold environment pathogens hemolysis antimicrobial resistance Microbiology QR1-502 |
spellingShingle |
Arctic extremely cold environment pathogens hemolysis antimicrobial resistance Microbiology QR1-502 Diana C. Mogrovejo Laura Perini Cene Gostinčar Kristina Sepčić Martina Turk Jerneja Ambrožič-Avguštin Florian H. H. Brill Nina Gunde-Cimerman Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria |
topic_facet |
Arctic extremely cold environment pathogens hemolysis antimicrobial resistance Microbiology QR1-502 |
description |
Many Arctic biomes, which are populated with abundant and diverse microbial life, are under threat: climate change and warming temperatures have raised concerns about diversity loss and possible emergence of pathogenic microorganisms. At present, there is little information on the occurrence of Arctic virulence-associated phenotypes. In this study we worked with 118 strains of bacteria (from 10 sampling sites in the Arctic region, located in Greenland and the Svalbard Archipelago) isolated using R2A medium. These strains belong to 4 phyla and represent 36 different bacterial genera. Phenotypic resistance to 8 clinically important antimicrobials (ampicillin, chloramphenicol, ciprofloxacin, cefotaxime, erythromycin, imipenem, kanamycin, and tetracycline) and thermotolerance range were determined. In addition, a screening of all isolates on blood agar media and erythrocytes suspension of bovine and sheep erythrocytes for virulence-linked hemolytic activity was performed. Although antimicrobial resistance profiles varied among the isolates, they were consistent within bacterial families and genera. Interestingly, a high number of isolates (83/104) were resistant to the tested concentration of imipenem (4 mg/L). In addition, one third of the isolates showed hemolytic activity on blood agar, however, in only 5% of the isolates hemolytic activity was also observed in the cell extracts when added to erythrocyte suspensions for 60 min. The observed microbial phenotypes contribute to our understanding of the presence of virulence-associated factors in the Arctic environments, while highlighting the potential risks associated with changes in the polar areas in the light of climate change. |
format |
Article in Journal/Newspaper |
author |
Diana C. Mogrovejo Laura Perini Cene Gostinčar Kristina Sepčić Martina Turk Jerneja Ambrožič-Avguštin Florian H. H. Brill Nina Gunde-Cimerman |
author_facet |
Diana C. Mogrovejo Laura Perini Cene Gostinčar Kristina Sepčić Martina Turk Jerneja Ambrožič-Avguštin Florian H. H. Brill Nina Gunde-Cimerman |
author_sort |
Diana C. Mogrovejo |
title |
Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria |
title_short |
Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria |
title_full |
Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria |
title_fullStr |
Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria |
title_full_unstemmed |
Prevalence of Antimicrobial Resistance and Hemolytic Phenotypes in Culturable Arctic Bacteria |
title_sort |
prevalence of antimicrobial resistance and hemolytic phenotypes in culturable arctic bacteria |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.00570 https://doaj.org/article/4b85800203e544bfbeadeb6d9e00b60f |
geographic |
Arctic Svalbard Svalbard Archipelago Greenland |
geographic_facet |
Arctic Svalbard Svalbard Archipelago Greenland |
genre |
Arctic Climate change Greenland Svalbard |
genre_facet |
Arctic Climate change Greenland Svalbard |
op_source |
Frontiers in Microbiology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00570/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00570 https://doaj.org/article/4b85800203e544bfbeadeb6d9e00b60f |
op_doi |
https://doi.org/10.3389/fmicb.2020.00570 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
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1766313200825925632 |