In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume
Objective: To investigate the fungistatic activity and synergistic effects of natural products and their constituents, including traditional oriental medicines (TOMs). Methods: Fungistatic activities of TOMs prepared by hot-water (115 °C) or ethanol (70%; 40 °C) extraction were determined by their m...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:4b6f32d264114b9a90518114612b3fbe 2023-05-15T15:12:35+02:00 In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume Young Soo Kim Jin Yeul Ma 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.225017 https://doaj.org/article/4b6f32d264114b9a90518114612b3fbe EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=109;epage=115;aulast=Kim https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.225017 https://doaj.org/article/4b6f32d264114b9a90518114612b3fbe Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 2, Pp 109-115 (2018) fungistatic activity hot-water and ethanol extracts traditional oriental medicine tinea trichophyton rubrum synergistic effect Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.225017 2022-12-31T01:36:41Z Objective: To investigate the fungistatic activity and synergistic effects of natural products and their constituents, including traditional oriental medicines (TOMs). Methods: Fungistatic activities of TOMs prepared by hot-water (115 °C) or ethanol (70%; 40 °C) extraction were determined by their minimum inhibitory concentration. To assess possible synergistic effects, minimum inhibitory concentrations of various combinations were evaluated. Results: By evaluating antifungal susceptibility of Trichophyton rubrum, which is a major causative fungus for several types of dermatophytosis, we confirmed that ethanol extracts were more active than hot-water extracts in 25 of the 36 TOMs, suggesting that the constituents with high hydrophobicity tend to contribute significantly to fungistatic activity. We selected four TOMs with high fungistatic activity, including Aucklandiae radix, Gentianae macrophyllae radix, Scutellariae radix, and Galla rhois, and their synergistic effects were investigated through the combination studies between TOMs or TOM-conventional drug terbinafine. In combinations between four TOMs, partial synergistic effects were observed in Aucklandiae radix–Galla rhois and Gentianae macrophyllae radix–Galla rhois combinations, as supported by the lowest fractional inhibitory concentration index value of 0.66 for both combinations. Furthermore, Galla rhois showed the strongest synergistic effect on growth inhibition of Trichophyton rubrum with a fractional inhibitory concentration index value of 0.50 in combination with terbinafine. Conclusions: Our findings indicate that the combination of TOMs and TOM-terbinafine may be effective on treatment for chronic and recurrent dermatophytosis by improving fungistatic activity and led to decrease systemic toxicity in clinical practice. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 2 109 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fungistatic activity hot-water and ethanol extracts traditional oriental medicine tinea trichophyton rubrum synergistic effect Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
fungistatic activity hot-water and ethanol extracts traditional oriental medicine tinea trichophyton rubrum synergistic effect Arctic medicine. Tropical medicine RC955-962 Young Soo Kim Jin Yeul Ma In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume |
topic_facet |
fungistatic activity hot-water and ethanol extracts traditional oriental medicine tinea trichophyton rubrum synergistic effect Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the fungistatic activity and synergistic effects of natural products and their constituents, including traditional oriental medicines (TOMs). Methods: Fungistatic activities of TOMs prepared by hot-water (115 °C) or ethanol (70%; 40 °C) extraction were determined by their minimum inhibitory concentration. To assess possible synergistic effects, minimum inhibitory concentrations of various combinations were evaluated. Results: By evaluating antifungal susceptibility of Trichophyton rubrum, which is a major causative fungus for several types of dermatophytosis, we confirmed that ethanol extracts were more active than hot-water extracts in 25 of the 36 TOMs, suggesting that the constituents with high hydrophobicity tend to contribute significantly to fungistatic activity. We selected four TOMs with high fungistatic activity, including Aucklandiae radix, Gentianae macrophyllae radix, Scutellariae radix, and Galla rhois, and their synergistic effects were investigated through the combination studies between TOMs or TOM-conventional drug terbinafine. In combinations between four TOMs, partial synergistic effects were observed in Aucklandiae radix–Galla rhois and Gentianae macrophyllae radix–Galla rhois combinations, as supported by the lowest fractional inhibitory concentration index value of 0.66 for both combinations. Furthermore, Galla rhois showed the strongest synergistic effect on growth inhibition of Trichophyton rubrum with a fractional inhibitory concentration index value of 0.50 in combination with terbinafine. Conclusions: Our findings indicate that the combination of TOMs and TOM-terbinafine may be effective on treatment for chronic and recurrent dermatophytosis by improving fungistatic activity and led to decrease systemic toxicity in clinical practice. |
format |
Article in Journal/Newspaper |
author |
Young Soo Kim Jin Yeul Ma |
author_facet |
Young Soo Kim Jin Yeul Ma |
author_sort |
Young Soo Kim |
title |
In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume |
title_short |
In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume |
title_full |
In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume |
title_fullStr |
In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume |
title_full_unstemmed |
In vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against Trichophyton rubrume |
title_sort |
in vitro fungistatic activity of 36 traditional oriental medicines and their synergistic effect against trichophyton rubrume |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.225017 https://doaj.org/article/4b6f32d264114b9a90518114612b3fbe |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 2, Pp 109-115 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=109;epage=115;aulast=Kim https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.225017 https://doaj.org/article/4b6f32d264114b9a90518114612b3fbe |
op_doi |
https://doi.org/10.4103/1995-7645.225017 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
2 |
container_start_page |
109 |
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1766343253134671872 |