Chicken eggs as a surveillance tool for malaria and leishmaniasis vector presence

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Rarissa de Oliveira e Silva, Maria Edilene Martins de Almeida, Eric Fabrício Marialva, Antônio Alcirley da Silva Balieiro, Diogo Pereira de Castro, Cláudia Maria Rios-Velasquez, Luis André Morais Mariúba, Felipe Arley Costa Pessoa
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2019
• Subjects: Lutzomyia longipalpis; Migonemyia migonei; Anopheles aquasalis; Sentinel surveillance; Chicken; Salivary proteins; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/0037-8682-0415-2018; https://doaj.org/article/4b4a2157c172481481de9512eebfbc63

Abstract INTRODUCTION: Malaria and leishmaniases are transmitted by vectors during blood-feeding. Vector-infected animals develop antibodies against the vector’s saliva. This study evaluated IgY antibody detection in the chicken eggs exposed to bites from Migonemyia migonei, Lutzomyia longipalpis and Anopheles aquasalis. METHODS: We used ELISA to quantify the antibody levels in the sera and exposed chicken eggs. RESULTS: High IgY levels were observed following immunization; furthermore, higher reactivity was observed in the eggs and species-specific immune response was observed post final immunization. CONCLUSIONS: Chicken eggs can be used as sentinels to surveil vector saliva antibodies.