Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance
ABSTRACT The methicillin resistant Staphylococcus aureus (MRSA) is recognized by its ability to acquire and transferring resistance genes through interspecies conjugative plasmids. However, transference of plasmids from Gram-positive cocci to Gram-negative bacilli is not well characterized. In this...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:4b4160f3e9ac428ca186af8e6b285063 2024-09-09T19:27:29+00:00 Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance Taniela Marli Bes Debora Satie Nagano Ana Paula Marchi Gaspar Camilo Lauro Vieira Perdigão-Neto Roberta Ruedas Martins Anna Sara Levin Silvia Figueiredo Costa 2021-12-01T00:00:00Z https://doi.org/10.1590/s1678-9946202163082 https://doaj.org/article/4b4160f3e9ac428ca186af8e6b285063 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100249&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163082 https://doaj.org/article/4b4160f3e9ac428ca186af8e6b285063 Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) Chlorhexidine resistance Conjugation Conjugative plasmids qacA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1590/s1678-9946202163082 2024-08-05T17:49:30Z ABSTRACT The methicillin resistant Staphylococcus aureus (MRSA) is recognized by its ability to acquire and transferring resistance genes through interspecies conjugative plasmids. However, transference of plasmids from Gram-positive cocci to Gram-negative bacilli is not well characterized. In this report, we describe the transfer of a conjugative plasmid carrying qacA from MRSA to Escherichia coli C600. We performed a conjugation experiment using a chlorhexidine resistant MRSA isolate (ST-105/SCCmec type III) carrying the gene qacA and qacC as the donor and a chlorhexidine susceptible E. coli C600 isolate as the receptor. Transconjugants were selected using MacConkey agar plates containing chlorhexidine in concentrations ranging from 0.25 to 16 g.L-1. To genotypically confirm the transfer of the resistance gene, the transconjugants were screened by Polymerase Chain Reaction (PCR) and submitted to Sanger’s sequencing. MRSA isolates successfully transferred the chlorhexidine resistance gene (qacA) to the recipient E. coli strain C600. The E. coli transconjugant exhibited an important reduction of chlorhexidine susceptibility, with MICs increasing from ≤ 0.25 to ≥ 16 g.L-1 after conjugation. The qacA gene was detected by PCR as well as in the Sanger’s sequencing analysis of DNA from transconjugant plasmids. To the best of our knowledge, this is the first report of the plasmid p_8N_qac(MN687830.1) carrying qacA and its transfer by conjugation from a MRSA to an E. coli. These findings increase concerns on the emergence of resistance dissemination across the genus and emphasizes the importance of continuous antiseptic stewardship. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 63 |
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Chlorhexidine resistance Conjugation Conjugative plasmids qacA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Chlorhexidine resistance Conjugation Conjugative plasmids qacA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Taniela Marli Bes Debora Satie Nagano Ana Paula Marchi Gaspar Camilo Lauro Vieira Perdigão-Neto Roberta Ruedas Martins Anna Sara Levin Silvia Figueiredo Costa Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance |
topic_facet |
Chlorhexidine resistance Conjugation Conjugative plasmids qacA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT The methicillin resistant Staphylococcus aureus (MRSA) is recognized by its ability to acquire and transferring resistance genes through interspecies conjugative plasmids. However, transference of plasmids from Gram-positive cocci to Gram-negative bacilli is not well characterized. In this report, we describe the transfer of a conjugative plasmid carrying qacA from MRSA to Escherichia coli C600. We performed a conjugation experiment using a chlorhexidine resistant MRSA isolate (ST-105/SCCmec type III) carrying the gene qacA and qacC as the donor and a chlorhexidine susceptible E. coli C600 isolate as the receptor. Transconjugants were selected using MacConkey agar plates containing chlorhexidine in concentrations ranging from 0.25 to 16 g.L-1. To genotypically confirm the transfer of the resistance gene, the transconjugants were screened by Polymerase Chain Reaction (PCR) and submitted to Sanger’s sequencing. MRSA isolates successfully transferred the chlorhexidine resistance gene (qacA) to the recipient E. coli strain C600. The E. coli transconjugant exhibited an important reduction of chlorhexidine susceptibility, with MICs increasing from ≤ 0.25 to ≥ 16 g.L-1 after conjugation. The qacA gene was detected by PCR as well as in the Sanger’s sequencing analysis of DNA from transconjugant plasmids. To the best of our knowledge, this is the first report of the plasmid p_8N_qac(MN687830.1) carrying qacA and its transfer by conjugation from a MRSA to an E. coli. These findings increase concerns on the emergence of resistance dissemination across the genus and emphasizes the importance of continuous antiseptic stewardship. |
format |
Article in Journal/Newspaper |
author |
Taniela Marli Bes Debora Satie Nagano Ana Paula Marchi Gaspar Camilo Lauro Vieira Perdigão-Neto Roberta Ruedas Martins Anna Sara Levin Silvia Figueiredo Costa |
author_facet |
Taniela Marli Bes Debora Satie Nagano Ana Paula Marchi Gaspar Camilo Lauro Vieira Perdigão-Neto Roberta Ruedas Martins Anna Sara Levin Silvia Figueiredo Costa |
author_sort |
Taniela Marli Bes |
title |
Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance |
title_short |
Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance |
title_full |
Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance |
title_fullStr |
Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance |
title_full_unstemmed |
Conjugative transfer of plasmid p_8N_qac(MN687830.1) carrying qacA gene from Staphylococcus aureus to Escherichia coli C600: potential mechanism for spreading chlorhexidine resistance |
title_sort |
conjugative transfer of plasmid p_8n_qac(mn687830.1) carrying qaca gene from staphylococcus aureus to escherichia coli c600: potential mechanism for spreading chlorhexidine resistance |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2021 |
url |
https://doi.org/10.1590/s1678-9946202163082 https://doaj.org/article/4b4160f3e9ac428ca186af8e6b285063 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100249&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163082 https://doaj.org/article/4b4160f3e9ac428ca186af8e6b285063 |
op_doi |
https://doi.org/10.1590/s1678-9946202163082 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
63 |
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1809896906968530944 |