Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin
Leprosy is a disease caused by Mycobacterium leprae that carries a high risk of disability, making early diagnosis mandatory. This study aimed to determine the applicability of anti-PGL-1 IgM antibody detection, using the ML FLOW technique, as an assistant tool for the detection of leprosy infection...
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ftdoajarticles:oai:doaj.org/article:4b1de30593fa47bc8a2dec68c07869bf 2024-09-09T19:28:27+00:00 Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin Omesina Maroja Limeira Ciro Martins Gomes Orlando Oliveira de Morais Mariana Vicente Cesetti Rosicler Rocha Aiza Alvarez 2013-06-01T00:00:00Z https://doaj.org/article/4b1de30593fa47bc8a2dec68c07869bf EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000300173 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/4b1de30593fa47bc8a2dec68c07869bf Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 3, Pp 173-177 (2013) Leprosy Mycobacterium leprae Diagnosis Prevention & control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2013 ftdoajarticles 2024-08-05T17:49:30Z Leprosy is a disease caused by Mycobacterium leprae that carries a high risk of disability, making early diagnosis mandatory. This study aimed to determine the applicability of anti-PGL-1 IgM antibody detection, using the ML FLOW technique, as an assistant tool for the detection of leprosy infection in asymptomatic household contacts (AHHC) of multibacillary leprosy index cases from Midwest Brazil. Serological changes induced by the prophylaxis of these household contacts with Bacillus Calmette-Guérin (BCG) were also verified. A total of 91 AHHC were assessed, among which, 18.68% (n = 17) presented both positive bacilloscopy and positive anti-PGL-1 IgM serology. Positivity concordance between these two laboratorial exams (Kappa Index = 1; p < 0.001) was indicated, however, one case did not demonstrate concordance between the semiquantitative assessment of anti-PGL-1 IgM and the bacilloscopy index (Kappa Index = 0.96; p < 0.001). Among the 17 AHHC with positive bacilloscopy, eight were reassessed after prophylaxis with BCG and two of them presented negative anti-PGL-1 IgM serology, being these patients who had presented a bacilloscopy index of < 2[+] in the initial assessment. This study shows that anti-PGL-1 IgM detection may be used as a tool to determine the bacillary load in AHHC and to detect immune changes related to prophylaxis by nonspecific vaccination. A hanseníase é doença causada pelo Mycobacterium leprae, apresentando elevado potencial incapacitante, o que torna indispensável seu diagnóstico precoce. O estudo visa determinar a aplicabilidade da detecção de anticorpos anti-PGL1-IgM por meio da técnica do ML FLOW como ferramenta adjuvante ao diagnóstico de hanseníase em contatos domiciliares assintomáticos (AHHC) de pacientes multibacilares procedentes da região Centro-Oeste do Brasil, bem como, documentar o comportamento sorológico após a profilaxia com a vacina Bacillus Calmette-Guérin (BCG). Foram avaliados 91 AHHC atendidos no Hospital Universitário de Brasília - Brasil, dos quais 18,68% ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Elevado ENVELOPE(-63.267,-63.267,-64.683,-64.683) Torna ENVELOPE(44.209,44.209,68.045,68.045) Calmette ENVELOPE(-67.150,-67.150,-68.050,-68.050) |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Leprosy Mycobacterium leprae Diagnosis Prevention & control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Leprosy Mycobacterium leprae Diagnosis Prevention & control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Omesina Maroja Limeira Ciro Martins Gomes Orlando Oliveira de Morais Mariana Vicente Cesetti Rosicler Rocha Aiza Alvarez Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin |
topic_facet |
Leprosy Mycobacterium leprae Diagnosis Prevention & control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Leprosy is a disease caused by Mycobacterium leprae that carries a high risk of disability, making early diagnosis mandatory. This study aimed to determine the applicability of anti-PGL-1 IgM antibody detection, using the ML FLOW technique, as an assistant tool for the detection of leprosy infection in asymptomatic household contacts (AHHC) of multibacillary leprosy index cases from Midwest Brazil. Serological changes induced by the prophylaxis of these household contacts with Bacillus Calmette-Guérin (BCG) were also verified. A total of 91 AHHC were assessed, among which, 18.68% (n = 17) presented both positive bacilloscopy and positive anti-PGL-1 IgM serology. Positivity concordance between these two laboratorial exams (Kappa Index = 1; p < 0.001) was indicated, however, one case did not demonstrate concordance between the semiquantitative assessment of anti-PGL-1 IgM and the bacilloscopy index (Kappa Index = 0.96; p < 0.001). Among the 17 AHHC with positive bacilloscopy, eight were reassessed after prophylaxis with BCG and two of them presented negative anti-PGL-1 IgM serology, being these patients who had presented a bacilloscopy index of < 2[+] in the initial assessment. This study shows that anti-PGL-1 IgM detection may be used as a tool to determine the bacillary load in AHHC and to detect immune changes related to prophylaxis by nonspecific vaccination. A hanseníase é doença causada pelo Mycobacterium leprae, apresentando elevado potencial incapacitante, o que torna indispensável seu diagnóstico precoce. O estudo visa determinar a aplicabilidade da detecção de anticorpos anti-PGL1-IgM por meio da técnica do ML FLOW como ferramenta adjuvante ao diagnóstico de hanseníase em contatos domiciliares assintomáticos (AHHC) de pacientes multibacilares procedentes da região Centro-Oeste do Brasil, bem como, documentar o comportamento sorológico após a profilaxia com a vacina Bacillus Calmette-Guérin (BCG). Foram avaliados 91 AHHC atendidos no Hospital Universitário de Brasília - Brasil, dos quais 18,68% ... |
format |
Article in Journal/Newspaper |
author |
Omesina Maroja Limeira Ciro Martins Gomes Orlando Oliveira de Morais Mariana Vicente Cesetti Rosicler Rocha Aiza Alvarez |
author_facet |
Omesina Maroja Limeira Ciro Martins Gomes Orlando Oliveira de Morais Mariana Vicente Cesetti Rosicler Rocha Aiza Alvarez |
author_sort |
Omesina Maroja Limeira |
title |
Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin |
title_short |
Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin |
title_full |
Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin |
title_fullStr |
Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin |
title_full_unstemmed |
Active search for leprosy cases in Midwestern Brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus Calmette-Guérin Busca ativa por casos de hanseníase no Centro-Oeste do Brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com Bacillus Calmette-Guérin |
title_sort |
active search for leprosy cases in midwestern brazil: a serological evaluation of asymptomatic household contacts before and after prophylaxis with bacillus calmette-guérin busca ativa por casos de hanseníase no centro-oeste do brasil: avaliação sorológica dos contatos domiciliares assintomáticos antes e após a profilaxia com bacillus calmette-guérin |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2013 |
url |
https://doaj.org/article/4b1de30593fa47bc8a2dec68c07869bf |
long_lat |
ENVELOPE(-63.267,-63.267,-64.683,-64.683) ENVELOPE(44.209,44.209,68.045,68.045) ENVELOPE(-67.150,-67.150,-68.050,-68.050) |
geographic |
Arctic Elevado Torna Calmette |
geographic_facet |
Arctic Elevado Torna Calmette |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 3, Pp 173-177 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000300173 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/4b1de30593fa47bc8a2dec68c07869bf |
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1809897763871129600 |