Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes

BACKGROUND Hyperglycemia and oxidative stress cause phagocytosis dysfunction in patients with diabetes. A combination of lycopene and metformin can reduce oxidative stress and blood glucose. This study aimed to determine the effect of combined lycopene and metformin on phagocytosis function, glycate...

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Published in:Medical Journal of Indonesia
Main Authors: Medina Sianturi, Neni Susilaningsih, Heri Nugroho, Nyoman Suci, Tri Nur Kristina, Maria Suryani
Format: Article in Journal/Newspaper
Language:English
Published: Faculty of Medicine Universitas Indonesia 2023
Subjects:
DML
Online Access:https://doi.org/10.13181/mji.oa.236774
https://doaj.org/article/4b00ee1c1301440bb313d3f004bf4dae
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spelling ftdoajarticles:oai:doaj.org/article:4b00ee1c1301440bb313d3f004bf4dae 2023-08-20T04:06:09+02:00 Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes Medina Sianturi Neni Susilaningsih Heri Nugroho Nyoman Suci Tri Nur Kristina Maria Suryani 2023-07-01T00:00:00Z https://doi.org/10.13181/mji.oa.236774 https://doaj.org/article/4b00ee1c1301440bb313d3f004bf4dae EN eng Faculty of Medicine Universitas Indonesia http://mji.ui.ac.id/journal/index.php/mji/article/view/6774 https://doaj.org/toc/0853-1773 https://doaj.org/toc/2252-8083 doi:10.13181/mji.oa.236774 0853-1773 2252-8083 https://doaj.org/article/4b00ee1c1301440bb313d3f004bf4dae Medical Journal of Indonesia, Vol 1, Iss 1 (2023) glycemic control lycopene metformin oxidative stress phagocytosis Medicine (General) R5-920 article 2023 ftdoajarticles https://doi.org/10.13181/mji.oa.236774 2023-07-30T00:39:06Z BACKGROUND Hyperglycemia and oxidative stress cause phagocytosis dysfunction in patients with diabetes. A combination of lycopene and metformin can reduce oxidative stress and blood glucose. This study aimed to determine the effect of combined lycopene and metformin on phagocytosis function, glycated hemoglobin A1c (HbA1c), nitric oxide (NO), reactive oxygen species (ROS), and advanced glycation end products (AGEs). METHODS A randomized controlled study was conducted in rats at the Center for Food and Nutrition Studies, Universitas Gadjah Mada, Yogyakarta, Indonesia, from August to September 2022. 30 rats were divided into control (n = 5) and type 2 diabetes mellitus (T2DM) (n = 25) groups. Rats in the T2DM group were induced by a high-fat diet combined with streptozotocin-nicotinamide. The 25 rats were then divided into five subgroups: 1 ml coconut oil (DM), 250 mg/kg metformin in 1 ml coconut oil (DMet), 250 mg/kg metformin + 10 mg/kg lycopene in 1 ml coconut oil (DML-10), 250 mg/kg metformin + 20 mg/kg lycopene in 1 ml coconut oil (DML-20), and 250 mg/kg metformin + 40 mg/kg lycopene in 1 ml coconut oil (DML-40). Treatments were administered daily for 4 weeks. The macrophage phagocytosis index (PI), HbA1c levels, ROS, NO, and AGEs serum were evaluated. RESULTS There was a significant difference in the PI, HbA1c, NO, ROS, and AGEs between the groups (p<0.001). The DML-20 and DML-40 groups had significantly increased PI and decreased NO, ROS, and AGEs levels than metformin alone (p<0.05). CONCLUSIONS Lycopene combined with metformin could improve phagocytosis function, glycemic control, and oxidative stress. Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles Medical Journal of Indonesia 32 1 1 6
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic glycemic control
lycopene
metformin
oxidative stress
phagocytosis
Medicine (General)
R5-920
spellingShingle glycemic control
lycopene
metformin
oxidative stress
phagocytosis
Medicine (General)
R5-920
Medina Sianturi
Neni Susilaningsih
Heri Nugroho
Nyoman Suci
Tri Nur Kristina
Maria Suryani
Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes
topic_facet glycemic control
lycopene
metformin
oxidative stress
phagocytosis
Medicine (General)
R5-920
description BACKGROUND Hyperglycemia and oxidative stress cause phagocytosis dysfunction in patients with diabetes. A combination of lycopene and metformin can reduce oxidative stress and blood glucose. This study aimed to determine the effect of combined lycopene and metformin on phagocytosis function, glycated hemoglobin A1c (HbA1c), nitric oxide (NO), reactive oxygen species (ROS), and advanced glycation end products (AGEs). METHODS A randomized controlled study was conducted in rats at the Center for Food and Nutrition Studies, Universitas Gadjah Mada, Yogyakarta, Indonesia, from August to September 2022. 30 rats were divided into control (n = 5) and type 2 diabetes mellitus (T2DM) (n = 25) groups. Rats in the T2DM group were induced by a high-fat diet combined with streptozotocin-nicotinamide. The 25 rats were then divided into five subgroups: 1 ml coconut oil (DM), 250 mg/kg metformin in 1 ml coconut oil (DMet), 250 mg/kg metformin + 10 mg/kg lycopene in 1 ml coconut oil (DML-10), 250 mg/kg metformin + 20 mg/kg lycopene in 1 ml coconut oil (DML-20), and 250 mg/kg metformin + 40 mg/kg lycopene in 1 ml coconut oil (DML-40). Treatments were administered daily for 4 weeks. The macrophage phagocytosis index (PI), HbA1c levels, ROS, NO, and AGEs serum were evaluated. RESULTS There was a significant difference in the PI, HbA1c, NO, ROS, and AGEs between the groups (p<0.001). The DML-20 and DML-40 groups had significantly increased PI and decreased NO, ROS, and AGEs levels than metformin alone (p<0.05). CONCLUSIONS Lycopene combined with metformin could improve phagocytosis function, glycemic control, and oxidative stress.
format Article in Journal/Newspaper
author Medina Sianturi
Neni Susilaningsih
Heri Nugroho
Nyoman Suci
Tri Nur Kristina
Maria Suryani
author_facet Medina Sianturi
Neni Susilaningsih
Heri Nugroho
Nyoman Suci
Tri Nur Kristina
Maria Suryani
author_sort Medina Sianturi
title Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes
title_short Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes
title_full Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes
title_fullStr Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes
title_full_unstemmed Effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes
title_sort effect of lycopene and metformin combination on phagocytosis, glycemic control, and oxidative stress in rats with type 2 diabetes
publisher Faculty of Medicine Universitas Indonesia
publishDate 2023
url https://doi.org/10.13181/mji.oa.236774
https://doaj.org/article/4b00ee1c1301440bb313d3f004bf4dae
genre DML
genre_facet DML
op_source Medical Journal of Indonesia, Vol 1, Iss 1 (2023)
op_relation http://mji.ui.ac.id/journal/index.php/mji/article/view/6774
https://doaj.org/toc/0853-1773
https://doaj.org/toc/2252-8083
doi:10.13181/mji.oa.236774
0853-1773
2252-8083
https://doaj.org/article/4b00ee1c1301440bb313d3f004bf4dae
op_doi https://doi.org/10.13181/mji.oa.236774
container_title Medical Journal of Indonesia
container_volume 32
container_issue 1
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