A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation.
The cestode Echinococcus granulosus--the agent of cystic echinococcosis, a zoonosis affecting humans and domestic animals worldwide--is an excellent model for the study of host-parasite cross-talk that interfaces with two mammalian hosts. To develop the molecular analysis of these interactions, we c...
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ftdoajarticles:oai:doaj.org/article:4afee9c839974efd9a80f36ade1a1881 2023-05-15T15:16:02+02:00 A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation. John Parkinson James D Wasmuth Gustavo Salinas Cristiano V Bizarro Chris Sanford Matthew Berriman Henrique B Ferreira Arnaldo Zaha Mark L Blaxter Rick M Maizels Cecilia Fernández 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001897 https://doaj.org/article/4afee9c839974efd9a80f36ade1a1881 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3510090?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001897 https://doaj.org/article/4afee9c839974efd9a80f36ade1a1881 PLoS Neglected Tropical Diseases, Vol 6, Iss 11, p e1897 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001897 2022-12-30T21:15:58Z The cestode Echinococcus granulosus--the agent of cystic echinococcosis, a zoonosis affecting humans and domestic animals worldwide--is an excellent model for the study of host-parasite cross-talk that interfaces with two mammalian hosts. To develop the molecular analysis of these interactions, we carried out an EST survey of E. granulosus larval stages. We report the salient features of this study with a focus on genes reflecting physiological adaptations of different parasite stages.We generated ~10,000 ESTs from two sets of full-length enriched libraries (derived from oligo-capped and trans-spliced cDNAs) prepared with three parasite materials: hydatid cyst wall, larval worms (protoscoleces), and pepsin/H(+)-activated protoscoleces. The ESTs were clustered into 2700 distinct gene products. In the context of the biology of E. granulosus, our analyses reveal: (i) a diverse group of abundant long non-protein coding transcripts showing homology to a middle repetitive element (EgBRep) that could either be active molecular species or represent precursors of small RNAs (like piRNAs); (ii) an up-regulation of fermentative pathways in the tissue of the cyst wall; (iii) highly expressed thiol- and selenol-dependent antioxidant enzyme targets of thioredoxin glutathione reductase, the functional hub of redox metabolism in parasitic flatworms; (iv) candidate apomucins for the external layer of the tissue-dwelling hydatid cyst, a mucin-rich structure that is critical for survival in the intermediate host; (v) a set of tetraspanins, a protein family that appears to have expanded in the cestode lineage; and (vi) a set of platyhelminth-specific gene products that may offer targets for novel pan-platyhelminth drug development.This survey has greatly increased the quality and the quantity of the molecular information on E. granulosus and constitutes a valuable resource for gene prediction on the parasite genome and for further genomic and proteomic analyses focused on cestodes and platyhelminths. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 11 e1897 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 John Parkinson James D Wasmuth Gustavo Salinas Cristiano V Bizarro Chris Sanford Matthew Berriman Henrique B Ferreira Arnaldo Zaha Mark L Blaxter Rick M Maizels Cecilia Fernández A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The cestode Echinococcus granulosus--the agent of cystic echinococcosis, a zoonosis affecting humans and domestic animals worldwide--is an excellent model for the study of host-parasite cross-talk that interfaces with two mammalian hosts. To develop the molecular analysis of these interactions, we carried out an EST survey of E. granulosus larval stages. We report the salient features of this study with a focus on genes reflecting physiological adaptations of different parasite stages.We generated ~10,000 ESTs from two sets of full-length enriched libraries (derived from oligo-capped and trans-spliced cDNAs) prepared with three parasite materials: hydatid cyst wall, larval worms (protoscoleces), and pepsin/H(+)-activated protoscoleces. The ESTs were clustered into 2700 distinct gene products. In the context of the biology of E. granulosus, our analyses reveal: (i) a diverse group of abundant long non-protein coding transcripts showing homology to a middle repetitive element (EgBRep) that could either be active molecular species or represent precursors of small RNAs (like piRNAs); (ii) an up-regulation of fermentative pathways in the tissue of the cyst wall; (iii) highly expressed thiol- and selenol-dependent antioxidant enzyme targets of thioredoxin glutathione reductase, the functional hub of redox metabolism in parasitic flatworms; (iv) candidate apomucins for the external layer of the tissue-dwelling hydatid cyst, a mucin-rich structure that is critical for survival in the intermediate host; (v) a set of tetraspanins, a protein family that appears to have expanded in the cestode lineage; and (vi) a set of platyhelminth-specific gene products that may offer targets for novel pan-platyhelminth drug development.This survey has greatly increased the quality and the quantity of the molecular information on E. granulosus and constitutes a valuable resource for gene prediction on the parasite genome and for further genomic and proteomic analyses focused on cestodes and platyhelminths. |
format |
Article in Journal/Newspaper |
author |
John Parkinson James D Wasmuth Gustavo Salinas Cristiano V Bizarro Chris Sanford Matthew Berriman Henrique B Ferreira Arnaldo Zaha Mark L Blaxter Rick M Maizels Cecilia Fernández |
author_facet |
John Parkinson James D Wasmuth Gustavo Salinas Cristiano V Bizarro Chris Sanford Matthew Berriman Henrique B Ferreira Arnaldo Zaha Mark L Blaxter Rick M Maizels Cecilia Fernández |
author_sort |
John Parkinson |
title |
A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation. |
title_short |
A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation. |
title_full |
A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation. |
title_fullStr |
A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation. |
title_full_unstemmed |
A transcriptomic analysis of Echinococcus granulosus larval stages: implications for parasite biology and host adaptation. |
title_sort |
transcriptomic analysis of echinococcus granulosus larval stages: implications for parasite biology and host adaptation. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001897 https://doaj.org/article/4afee9c839974efd9a80f36ade1a1881 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 11, p e1897 (2012) |
op_relation |
http://europepmc.org/articles/PMC3510090?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001897 https://doaj.org/article/4afee9c839974efd9a80f36ade1a1881 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001897 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
11 |
container_start_page |
e1897 |
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1766346343354204160 |