Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3
The use of monoamine oxidases (MAOs) in amine oxidation is a great example of how biocatalysis can be applied in the agricultural or pharmaceutical industry and manufacturing of fine chemicals to make a shift from traditional chemical synthesis towards more sustainable green chemistry. This article...
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2021
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ftdoajarticles:oai:doaj.org/article:4afdaf00993640ffbe8951fadef65374 2023-05-15T13:59:09+02:00 Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3 Iga Jodłowska Aleksandra Twarda-Clapa Kamil Szymczak Aneta M. Białkowska 2021-10-01T00:00:00Z https://doi.org/10.3390/molecules26206237 https://doaj.org/article/4afdaf00993640ffbe8951fadef65374 EN eng MDPI AG https://www.mdpi.com/1420-3049/26/20/6237 https://doaj.org/toc/1420-3049 doi:10.3390/molecules26206237 1420-3049 https://doaj.org/article/4afdaf00993640ffbe8951fadef65374 Molecules, Vol 26, Iss 6237, p 6237 (2021) psychrophilic fungi monoamine oxidase oxidative deamination Organic chemistry QD241-441 article 2021 ftdoajarticles https://doi.org/10.3390/molecules26206237 2022-12-31T11:01:45Z The use of monoamine oxidases (MAOs) in amine oxidation is a great example of how biocatalysis can be applied in the agricultural or pharmaceutical industry and manufacturing of fine chemicals to make a shift from traditional chemical synthesis towards more sustainable green chemistry. This article reports the screening of fourteen Antarctic fungi strains for MAO activity and the discovery of a novel psychrozyme MAOP3 isolated from the Pseudogymnoascus sp. P3. The activity of the native enzyme was 1350 ± 10.5 U/L towards a primary ( n -butylamine) amine, and 1470 ± 10.6 U/L towards a secondary (6,6-dimethyl-3-azabicyclohexane) amine. MAO P3 has the potential for applications in biotransformations due to its wide substrate specificity (aliphatic and cyclic amines, pyrrolidine derivatives). The psychrozyme operates at an optimal temperature of 30 °C, retains 75% of activity at 20 °C, and is rather thermolabile, which is beneficial for a reduction in the overall costs of a bioprocess and offers a convenient way of heat inactivation. The reported biocatalyst is the first psychrophilic MAO; its unique biochemical properties, substrate specificity, and effectiveness predispose MAO P3 for use in environmentally friendly, low-emission biotransformations. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic Molecules 26 20 6237 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
psychrophilic fungi monoamine oxidase oxidative deamination Organic chemistry QD241-441 |
spellingShingle |
psychrophilic fungi monoamine oxidase oxidative deamination Organic chemistry QD241-441 Iga Jodłowska Aleksandra Twarda-Clapa Kamil Szymczak Aneta M. Białkowska Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3 |
topic_facet |
psychrophilic fungi monoamine oxidase oxidative deamination Organic chemistry QD241-441 |
description |
The use of monoamine oxidases (MAOs) in amine oxidation is a great example of how biocatalysis can be applied in the agricultural or pharmaceutical industry and manufacturing of fine chemicals to make a shift from traditional chemical synthesis towards more sustainable green chemistry. This article reports the screening of fourteen Antarctic fungi strains for MAO activity and the discovery of a novel psychrozyme MAOP3 isolated from the Pseudogymnoascus sp. P3. The activity of the native enzyme was 1350 ± 10.5 U/L towards a primary ( n -butylamine) amine, and 1470 ± 10.6 U/L towards a secondary (6,6-dimethyl-3-azabicyclohexane) amine. MAO P3 has the potential for applications in biotransformations due to its wide substrate specificity (aliphatic and cyclic amines, pyrrolidine derivatives). The psychrozyme operates at an optimal temperature of 30 °C, retains 75% of activity at 20 °C, and is rather thermolabile, which is beneficial for a reduction in the overall costs of a bioprocess and offers a convenient way of heat inactivation. The reported biocatalyst is the first psychrophilic MAO; its unique biochemical properties, substrate specificity, and effectiveness predispose MAO P3 for use in environmentally friendly, low-emission biotransformations. |
format |
Article in Journal/Newspaper |
author |
Iga Jodłowska Aleksandra Twarda-Clapa Kamil Szymczak Aneta M. Białkowska |
author_facet |
Iga Jodłowska Aleksandra Twarda-Clapa Kamil Szymczak Aneta M. Białkowska |
author_sort |
Iga Jodłowska |
title |
Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3 |
title_short |
Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3 |
title_full |
Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3 |
title_fullStr |
Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3 |
title_full_unstemmed |
Green Oxidation of Amines by a Novel Cold-Adapted Monoamine Oxidase MAO P3 from Psychrophilic Fungi Pseudogymnoascus sp. P3 |
title_sort |
green oxidation of amines by a novel cold-adapted monoamine oxidase mao p3 from psychrophilic fungi pseudogymnoascus sp. p3 |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/molecules26206237 https://doaj.org/article/4afdaf00993640ffbe8951fadef65374 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Molecules, Vol 26, Iss 6237, p 6237 (2021) |
op_relation |
https://www.mdpi.com/1420-3049/26/20/6237 https://doaj.org/toc/1420-3049 doi:10.3390/molecules26206237 1420-3049 https://doaj.org/article/4afdaf00993640ffbe8951fadef65374 |
op_doi |
https://doi.org/10.3390/molecules26206237 |
container_title |
Molecules |
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26 |
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20 |
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6237 |
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1766267604679262208 |