Integrating omics to characterize eco‐physiological adaptations: How moose diet and metabolism differ across biogeographic zones

• Published in: Ecology and Evolution
• Main Authors: Christian Fohringer, Ilona Dudka, Robert Spitzer, Fredrik Stenbacka, Olena Rzhepishevska, Joris P. G. M. Cromsigt, Gerhard Gröbner, Göran Ericsson, Navinder J. Singh
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2021
• Subjects: biomarker; DNA‐metabarcoding; energy metabolism; metabolomics; nutritional ecology; starvation; Ecology; QH540-549.5; Alces alces; Northern Sweden
• Online Access: https://doi.org/10.1002/ece3.7265; https://doaj.org/article/4afa598bb07d417e827357994d8aff2b

Abstract With accelerated land conversion and global heating at northern latitudes, it becomes crucial to understand, how life histories of animals in extreme environments adapt to these changes. Animals may either adapt by adjusting foraging behavior or through physiological responses, including adjusting their energy metabolism or both. Until now, it has been difficult to study such adaptations in free‐ranging animals due to methodological constraints that prevent extensive spatiotemporal coverage of ecological and physiological data. Through a novel approach of combining DNA‐metabarcoding and nuclear magnetic resonance (NMR)‐based metabolomics, we aim to elucidate the links between diets and metabolism in Scandinavian moose Alces alces over three biogeographic zones using a unique dataset of 265 marked individuals. Based on 17 diet items, we identified four different classes of diet types that match browse species availability in respective ecoregions in northern Sweden. Individuals in the boreal zone consumed predominantly pine and had the least diverse diets, while individuals with highest diet diversity occurred in the coastal areas. Males exhibited lower average diet diversity than females. We identified several molecular markers indicating metabolic constraints linked to diet constraints in terms of food availability during winter. While animals consuming pine had higher lipid, phospocholine, and glycerophosphocholine concentrations in their serum than other diet types, birch‐ and willow/aspen‐rich diets exhibit elevated concentrations of several amino acids. The individuals with highest diet diversity had increased levels of ketone bodies, indicating extensive periods of starvation for these individuals. Our results show how the adaptive capacity of moose at the eco‐physiological level varies over a large eco‐geographic scale and how it responds to land use pressures. In light of extensive ongoing climate and land use changes, these findings pave the way for future scenario building for animal adaptive ...