Component composition of essential oils and ultrastructure of secretory cells of resin channel needles Juniperus communis (Cupressaceae)

• Published in: Сибирский лесной журнал
• Main Authors: N. V. Gerling, V. V. Punegov, I. V. Gruzdev
• Format: Article in Journal/Newspaper
• Language: English; Russian
• Published: Russian Academy of Sciences, Siberian Branch Publishing House 2015
• Subjects: Juniperus communis; essential oil; ultrastructure; secretory cells; Forestry; SD1-669.5; The Needles; taiga
• Online Access: https://doi.org/10.15372/SJFS20150606; https://doaj.org/article/4aefafadd766450a95279f33189b7988

The results of determining the qualitative and quantitative composition of essential oil Juniperus communis, growing under the canopy of spruce blueberry sphagnum subzone middle taiga. Juniperus communis essential oil is liquid light yellow color. The content of essential oil was 0.46 % in shoots with needles. 37 substances of components identified. Mass fraction of components in the essential oil of Juniperus communis reached 89 %. The highest percentage of occupied fraction of monoterpenes (82.3 %), the proportion of sesquiterpenes less than 0.5 % of the total composition of essential oils, alcohols 3.5 and 0.7 % esters. In monoterpenes fraction predominant α-pinene (24.5–32.6 %), β-pinene (15–20.3 %) and α-phellandrene (6.4–8.8 %). Essential oil of Juniperus communis is characterized by high content of monoterpenoids in contrast to other conifers of the taiga zone. All stages of biosynthesis essential oils occur in the epithelial cells of the resin channel (terpenoidogennyh cells). An oval shape have epithelial cells of the resin channel needles in transverse sections the Juniperus communis, which is situated vacuole in the center. Large number of lipid globules (up to 40) noted in the hyaloplasm of explored cells. Leucoplasts surrounded by membranes of smooth endoplasmic reticulum in cross sections of epithelial cells in resin channel of juniper. Endoplasmic reticulum is poorly developed in epithelial cells, which corresponds to the low content of sesquiterpenes in the needles during the study period. Development of large leucoplasts and large number of mitochondria associated with predominance of synthesis monoterpenoids the in the epithelium cells resin channel.