The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench
Recently, several reports showed that n-alkanes were abundant in the hadal zone, suggesting that n-alkanes could be an important source of nutrients for microorganisms in hadal ecosystems. To date, most of the published studies on the microbial capacity to degrade hydrocarbons were conducted only at...
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ftdoajarticles:oai:doaj.org/article:4aecb1b56d194d27ad96723cfcd81098 2023-05-15T13:52:18+02:00 The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench Jiahua Wang Yan Zhang Ying Liu Zhe Xie Junwei Cao Hongcai Zhang Jie Liu Tianqiang Bao Congwen Sun Bilin Liu Yuli Wei Jiasong Fang 2023-03-01T00:00:00Z https://doi.org/10.3389/fmicb.2023.1108651 https://doaj.org/article/4aecb1b56d194d27ad96723cfcd81098 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1108651/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1108651 https://doaj.org/article/4aecb1b56d194d27ad96723cfcd81098 Frontiers in Microbiology, Vol 14 (2023) Trench sediment Venatorbacter n-alkane utilization environmental distribution extracellular macromolecule degradation Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.3389/fmicb.2023.1108651 2023-03-26T01:34:28Z Recently, several reports showed that n-alkanes were abundant in the hadal zone, suggesting that n-alkanes could be an important source of nutrients for microorganisms in hadal ecosystems. To date, most of the published studies on the microbial capacity to degrade hydrocarbons were conducted only at atmospheric temperature and pressure (0.1 MPa), and little is known about whether and which microbes could utilize n-alkanes at in situ environmental conditions in the hadal zone, including low temperature and high hydrostatic pressure (especially >30 MPa). In this study, a piezotolerant bacterium, strain C2-1, was isolated from a Mariana Trench sediment at depth of 5,800 m. Strain C2-1 was able to grow at in situ temperature (4°C) and pressure (58 MPa) with n-alkanes as the sole carbon source. Phylogenetically, strain C2-1 and related strains (TMPB967, ST750PaO-4, IMCC1826, and TTBP476) should be classified into the genus Venatorbacter. Metagenomic analysis using ~5,000 publicly available datasets showed that Venatorbacter has a wide environmental distribution in seawater (38), marine sediments (3), hydrothermal vent plumes (2), Antarctic ice (1), groundwater (13), and marine sponge ecosystems (1). Most Venatorbacter species are non-obligate n-alkane degraders that could utilize, at a minimal, C16−C18n-alkanes, as well as other different types of carbon substrates, including carbohydrates, amino acids, peptides, and phospholipids. The type II secretion system, extracellular proteases, phospholipase, and endonuclease of Venatorbacter species were robustly expressed in the metatranscriptomes of deep-sea hydrothermal vents, suggesting their important contribution to secondary productivity by degrading extracellular macromolecules. The identification of denitrifying genes suggested a genus-specific ecological potential that allowed Venatorbacter species to be active in anoxic environments, e.g., the oxygen-minimal zone (OMZ) and the deeply buried marine sediments. Our results show that Venatorbacter species are ... Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Microbiology 14 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Trench sediment Venatorbacter n-alkane utilization environmental distribution extracellular macromolecule degradation Microbiology QR1-502 |
spellingShingle |
Trench sediment Venatorbacter n-alkane utilization environmental distribution extracellular macromolecule degradation Microbiology QR1-502 Jiahua Wang Yan Zhang Ying Liu Zhe Xie Junwei Cao Hongcai Zhang Jie Liu Tianqiang Bao Congwen Sun Bilin Liu Yuli Wei Jiasong Fang The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench |
topic_facet |
Trench sediment Venatorbacter n-alkane utilization environmental distribution extracellular macromolecule degradation Microbiology QR1-502 |
description |
Recently, several reports showed that n-alkanes were abundant in the hadal zone, suggesting that n-alkanes could be an important source of nutrients for microorganisms in hadal ecosystems. To date, most of the published studies on the microbial capacity to degrade hydrocarbons were conducted only at atmospheric temperature and pressure (0.1 MPa), and little is known about whether and which microbes could utilize n-alkanes at in situ environmental conditions in the hadal zone, including low temperature and high hydrostatic pressure (especially >30 MPa). In this study, a piezotolerant bacterium, strain C2-1, was isolated from a Mariana Trench sediment at depth of 5,800 m. Strain C2-1 was able to grow at in situ temperature (4°C) and pressure (58 MPa) with n-alkanes as the sole carbon source. Phylogenetically, strain C2-1 and related strains (TMPB967, ST750PaO-4, IMCC1826, and TTBP476) should be classified into the genus Venatorbacter. Metagenomic analysis using ~5,000 publicly available datasets showed that Venatorbacter has a wide environmental distribution in seawater (38), marine sediments (3), hydrothermal vent plumes (2), Antarctic ice (1), groundwater (13), and marine sponge ecosystems (1). Most Venatorbacter species are non-obligate n-alkane degraders that could utilize, at a minimal, C16−C18n-alkanes, as well as other different types of carbon substrates, including carbohydrates, amino acids, peptides, and phospholipids. The type II secretion system, extracellular proteases, phospholipase, and endonuclease of Venatorbacter species were robustly expressed in the metatranscriptomes of deep-sea hydrothermal vents, suggesting their important contribution to secondary productivity by degrading extracellular macromolecules. The identification of denitrifying genes suggested a genus-specific ecological potential that allowed Venatorbacter species to be active in anoxic environments, e.g., the oxygen-minimal zone (OMZ) and the deeply buried marine sediments. Our results show that Venatorbacter species are ... |
format |
Article in Journal/Newspaper |
author |
Jiahua Wang Yan Zhang Ying Liu Zhe Xie Junwei Cao Hongcai Zhang Jie Liu Tianqiang Bao Congwen Sun Bilin Liu Yuli Wei Jiasong Fang |
author_facet |
Jiahua Wang Yan Zhang Ying Liu Zhe Xie Junwei Cao Hongcai Zhang Jie Liu Tianqiang Bao Congwen Sun Bilin Liu Yuli Wei Jiasong Fang |
author_sort |
Jiahua Wang |
title |
The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench |
title_short |
The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench |
title_full |
The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench |
title_fullStr |
The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench |
title_full_unstemmed |
The phylogeny and metabolic potentials of an n-alkane-degrading Venatorbacter bacterium isolated from deep-sea sediment of the Mariana Trench |
title_sort |
phylogeny and metabolic potentials of an n-alkane-degrading venatorbacter bacterium isolated from deep-sea sediment of the mariana trench |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1108651 https://doaj.org/article/4aecb1b56d194d27ad96723cfcd81098 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Microbiology, Vol 14 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1108651/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1108651 https://doaj.org/article/4aecb1b56d194d27ad96723cfcd81098 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1108651 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
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1766256590429618176 |