Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus.
Scedosporium apiospermum is part of the Pseudallescheria-Scedosporium complex. Peptidorhamnomannans (PRMs) are cell wall glycopeptides present in some fungi, and their structures have been characterized in S. apiospermum, S. prolificans and Sporothrix schenckii. Prior work shows that PRMs can intera...
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ftdoajarticles:oai:doaj.org/article:4ad0a971538446e5b7e0dff7f0a2f2bc 2023-05-15T15:05:28+02:00 Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. Livia C L Lopes Rodrigo Rollin-Pinheiro Allan J Guimarães Vera C B Bittencourt Luis R Martinez Wade Koba Sandra E Farias Joshua D Nosanchuk Eliana Barreto-Bergter 2010-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000853 https://doaj.org/article/4ad0a971538446e5b7e0dff7f0a2f2bc EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2957425?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000853 https://doaj.org/article/4ad0a971538446e5b7e0dff7f0a2f2bc PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e853 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000853 2022-12-31T12:31:09Z Scedosporium apiospermum is part of the Pseudallescheria-Scedosporium complex. Peptidorhamnomannans (PRMs) are cell wall glycopeptides present in some fungi, and their structures have been characterized in S. apiospermum, S. prolificans and Sporothrix schenckii. Prior work shows that PRMs can interact with host cells and that the glycopeptides are antigenic. In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo. The mAbs recognized a carbohydrate epitope on PRM. In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages. In a murine infection model, mice treated with antibodies to PRM died prior to control animals. Thus, PRM is involved in morphogenesis and the binding of this glycopeptide by mAbs enhanced the virulence of the fungus. Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 10 e853 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Livia C L Lopes Rodrigo Rollin-Pinheiro Allan J Guimarães Vera C B Bittencourt Luis R Martinez Wade Koba Sandra E Farias Joshua D Nosanchuk Eliana Barreto-Bergter Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Scedosporium apiospermum is part of the Pseudallescheria-Scedosporium complex. Peptidorhamnomannans (PRMs) are cell wall glycopeptides present in some fungi, and their structures have been characterized in S. apiospermum, S. prolificans and Sporothrix schenckii. Prior work shows that PRMs can interact with host cells and that the glycopeptides are antigenic. In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo. The mAbs recognized a carbohydrate epitope on PRM. In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages. In a murine infection model, mice treated with antibodies to PRM died prior to control animals. Thus, PRM is involved in morphogenesis and the binding of this glycopeptide by mAbs enhanced the virulence of the fungus. Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis. |
format |
Article in Journal/Newspaper |
author |
Livia C L Lopes Rodrigo Rollin-Pinheiro Allan J Guimarães Vera C B Bittencourt Luis R Martinez Wade Koba Sandra E Farias Joshua D Nosanchuk Eliana Barreto-Bergter |
author_facet |
Livia C L Lopes Rodrigo Rollin-Pinheiro Allan J Guimarães Vera C B Bittencourt Luis R Martinez Wade Koba Sandra E Farias Joshua D Nosanchuk Eliana Barreto-Bergter |
author_sort |
Livia C L Lopes |
title |
Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. |
title_short |
Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. |
title_full |
Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. |
title_fullStr |
Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. |
title_full_unstemmed |
Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. |
title_sort |
monoclonal antibodies against peptidorhamnomannans of scedosporium apiospermum enhance the pathogenicity of the fungus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000853 https://doaj.org/article/4ad0a971538446e5b7e0dff7f0a2f2bc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e853 (2010) |
op_relation |
http://europepmc.org/articles/PMC2957425?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000853 https://doaj.org/article/4ad0a971538446e5b7e0dff7f0a2f2bc |
op_doi |
https://doi.org/10.1371/journal.pntd.0000853 |
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PLoS Neglected Tropical Diseases |
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4 |
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10 |
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e853 |
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1766337171334103040 |