Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
Background Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature revie...
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ftdoajarticles:oai:doaj.org/article:4a9e2f514c6b4d98a854ba6b3e61102a 2023-05-15T15:13:49+02:00 Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. YuYen Chan Kimberly Fornace Lindsey Wu Benjamin F Arnold Jeffrey W Priest Diana L Martin Michelle A Chang Jackie Cook Gillian Stresman Chris Drakeley 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009457 https://doaj.org/article/4a9e2f514c6b4d98a854ba6b3e61102a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009457 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009457 https://doaj.org/article/4a9e2f514c6b4d98a854ba6b3e61102a PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009457 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009457 2022-12-31T03:58:30Z Background Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature review was conducted to describe the methods used to determine cut-off values for data generated by multiplex bead assays. Methodology/principal findings A search was conducted in PubMed that included articles published from January 2010 to January 2020, and 308 relevant articles were identified that included the terms "serology", "cut-offs", and "multiplex bead assays". After application of exclusion of articles not relevant to neglected tropical diseases (NTD), vaccine preventable diseases (VPD), or malaria, 55 articles were examined based on their relevance to NTD or VPD. The most frequently applied approaches to determine seropositivity included the use of presumed unexposed populations, mixture models, receiver operating curves (ROC), and international standards. Other methods included the use of quantiles, pre-exposed endemic cohorts, and visual inflection points. Conclusions/significance For disease control programmes, seropositivity is a practical and easily interpretable health metric but determining appropriate cut-offs for positivity can be challenging. Considerations for optimal cut-off approaches should include factors such as methods recommended by previous research, transmission dynamics, and the immunological backgrounds of the population. In the absence of international standards for estimating seropositivity in a population, the use of consistent methods that align with individual disease epidemiological data will improve comparability between settings and enable the assessment of changes over time. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0009457 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 YuYen Chan Kimberly Fornace Lindsey Wu Benjamin F Arnold Jeffrey W Priest Diana L Martin Michelle A Chang Jackie Cook Gillian Stresman Chris Drakeley Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature review was conducted to describe the methods used to determine cut-off values for data generated by multiplex bead assays. Methodology/principal findings A search was conducted in PubMed that included articles published from January 2010 to January 2020, and 308 relevant articles were identified that included the terms "serology", "cut-offs", and "multiplex bead assays". After application of exclusion of articles not relevant to neglected tropical diseases (NTD), vaccine preventable diseases (VPD), or malaria, 55 articles were examined based on their relevance to NTD or VPD. The most frequently applied approaches to determine seropositivity included the use of presumed unexposed populations, mixture models, receiver operating curves (ROC), and international standards. Other methods included the use of quantiles, pre-exposed endemic cohorts, and visual inflection points. Conclusions/significance For disease control programmes, seropositivity is a practical and easily interpretable health metric but determining appropriate cut-offs for positivity can be challenging. Considerations for optimal cut-off approaches should include factors such as methods recommended by previous research, transmission dynamics, and the immunological backgrounds of the population. In the absence of international standards for estimating seropositivity in a population, the use of consistent methods that align with individual disease epidemiological data will improve comparability between settings and enable the assessment of changes over time. |
format |
Article in Journal/Newspaper |
author |
YuYen Chan Kimberly Fornace Lindsey Wu Benjamin F Arnold Jeffrey W Priest Diana L Martin Michelle A Chang Jackie Cook Gillian Stresman Chris Drakeley |
author_facet |
YuYen Chan Kimberly Fornace Lindsey Wu Benjamin F Arnold Jeffrey W Priest Diana L Martin Michelle A Chang Jackie Cook Gillian Stresman Chris Drakeley |
author_sort |
YuYen Chan |
title |
Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. |
title_short |
Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. |
title_full |
Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. |
title_fullStr |
Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. |
title_full_unstemmed |
Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. |
title_sort |
determining seropositivity-a review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009457 https://doaj.org/article/4a9e2f514c6b4d98a854ba6b3e61102a |
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Arctic |
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Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009457 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009457 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009457 https://doaj.org/article/4a9e2f514c6b4d98a854ba6b3e61102a |
op_doi |
https://doi.org/10.1371/journal.pntd.0009457 |
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PLOS Neglected Tropical Diseases |
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15 |
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6 |
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e0009457 |
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