High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions.
Hepatitis E virus (HEV) infects a range of species, including humans, pigs, wild boars and deer. Zoonotic transmission may contribute to the high HEV seroprevalence in the human population of many countries. A novel divergent HEV from moose (Alces alces) in Sweden was recently identified by partial...
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ftdoajarticles:oai:doaj.org/article:4a79c702575043fa9e576a5a54517251 2023-05-15T13:13:43+02:00 High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions. Jay Lin Marie Karlsson Ann-Sophie Olofson Sándor Belák Jonas Malmsten Anne-Marie Dalin Frederik Widén Heléne Norder 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0122102 https://doaj.org/article/4a79c702575043fa9e576a5a54517251 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4408071?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0122102 https://doaj.org/article/4a79c702575043fa9e576a5a54517251 PLoS ONE, Vol 10, Iss 4, p e0122102 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0122102 2022-12-31T15:35:48Z Hepatitis E virus (HEV) infects a range of species, including humans, pigs, wild boars and deer. Zoonotic transmission may contribute to the high HEV seroprevalence in the human population of many countries. A novel divergent HEV from moose (Alces alces) in Sweden was recently identified by partial genome sequencing. Since only one strain was found, its classification within the HEV family, prevalence in moose and zoonotic potential was unclear. We therefore investigated samples from 231 moose in seven Swedish counties for HEV, and sequenced a near complete moose HEV genome. Phylogenetic analysis to classify this virus within the family Hepeviridae and to explore potential host specific determinants was performed.The HEV prevalence of moose was determined by PCR (marker for active infection) and serological assays (marker of past infection) of sera and 51 fecal samples from 231 Swedish moose. Markers of active and past infection were found in 67 (29%) animals, while 34 (15%) were positive for HEV RNA, 43 (19%) were seropositive for anti-HEV antibodies, and 10 (4%) had both markers. The number of young individuals positive for HEV RNA was larger than for older individuals, and the number of anti-HEV antibody positive individuals increased with age. The high throughput sequenced moose HEV genome was 35-60% identical to existing HEVs. Partial ORF1 sequences from 13 moose strains showed high similarity among them, forming a distinct monophyletic clade with a common ancestor to HEV genotype 1-6 group, which includes members known for zoonotic transmission.This study demonstrates a high frequency of HEV in moose in Sweden, with markers of current and past infection demonstrated in 30% of the animals. Moose is thus an important animal reservoir of HEV. The phylogenetic relationship demonstrated that the moose HEV belonged to the genotype 1-6 group, which includes strains that also infect humans, and therefore may signify a potential for zoonotic transmission of this HEV. Article in Journal/Newspaper Alces alces Directory of Open Access Journals: DOAJ Articles PLOS ONE 10 4 e0122102 |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Jay Lin Marie Karlsson Ann-Sophie Olofson Sándor Belák Jonas Malmsten Anne-Marie Dalin Frederik Widén Heléne Norder High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions. |
topic_facet |
Medicine R Science Q |
description |
Hepatitis E virus (HEV) infects a range of species, including humans, pigs, wild boars and deer. Zoonotic transmission may contribute to the high HEV seroprevalence in the human population of many countries. A novel divergent HEV from moose (Alces alces) in Sweden was recently identified by partial genome sequencing. Since only one strain was found, its classification within the HEV family, prevalence in moose and zoonotic potential was unclear. We therefore investigated samples from 231 moose in seven Swedish counties for HEV, and sequenced a near complete moose HEV genome. Phylogenetic analysis to classify this virus within the family Hepeviridae and to explore potential host specific determinants was performed.The HEV prevalence of moose was determined by PCR (marker for active infection) and serological assays (marker of past infection) of sera and 51 fecal samples from 231 Swedish moose. Markers of active and past infection were found in 67 (29%) animals, while 34 (15%) were positive for HEV RNA, 43 (19%) were seropositive for anti-HEV antibodies, and 10 (4%) had both markers. The number of young individuals positive for HEV RNA was larger than for older individuals, and the number of anti-HEV antibody positive individuals increased with age. The high throughput sequenced moose HEV genome was 35-60% identical to existing HEVs. Partial ORF1 sequences from 13 moose strains showed high similarity among them, forming a distinct monophyletic clade with a common ancestor to HEV genotype 1-6 group, which includes members known for zoonotic transmission.This study demonstrates a high frequency of HEV in moose in Sweden, with markers of current and past infection demonstrated in 30% of the animals. Moose is thus an important animal reservoir of HEV. The phylogenetic relationship demonstrated that the moose HEV belonged to the genotype 1-6 group, which includes strains that also infect humans, and therefore may signify a potential for zoonotic transmission of this HEV. |
format |
Article in Journal/Newspaper |
author |
Jay Lin Marie Karlsson Ann-Sophie Olofson Sándor Belák Jonas Malmsten Anne-Marie Dalin Frederik Widén Heléne Norder |
author_facet |
Jay Lin Marie Karlsson Ann-Sophie Olofson Sándor Belák Jonas Malmsten Anne-Marie Dalin Frederik Widén Heléne Norder |
author_sort |
Jay Lin |
title |
High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions. |
title_short |
High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions. |
title_full |
High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions. |
title_fullStr |
High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions. |
title_full_unstemmed |
High prevalence of hepatitis e virus in Swedish moose--a phylogenetic characterization and comparison of the virus from different regions. |
title_sort |
high prevalence of hepatitis e virus in swedish moose--a phylogenetic characterization and comparison of the virus from different regions. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0122102 https://doaj.org/article/4a79c702575043fa9e576a5a54517251 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
PLoS ONE, Vol 10, Iss 4, p e0122102 (2015) |
op_relation |
http://europepmc.org/articles/PMC4408071?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0122102 https://doaj.org/article/4a79c702575043fa9e576a5a54517251 |
op_doi |
https://doi.org/10.1371/journal.pone.0122102 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
4 |
container_start_page |
e0122102 |
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1766260033464565760 |