Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon.
Ionizing radiation is a recognized genotoxic agent, however, little is known about the role of the functional form of DNA in these processes. Post translational modifications on histone proteins control the organization of chromatin and hence control transcriptional responses that ultimately affect...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0212123 https://doaj.org/article/4a1ddb464dd9409cad2c7250dc601439 |
id |
ftdoajarticles:oai:doaj.org/article:4a1ddb464dd9409cad2c7250dc601439 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:4a1ddb464dd9409cad2c7250dc601439 2023-05-15T15:31:24+02:00 Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon. Leif Christopher Lindeman Jorke Harmen Kamstra Jarle Ballangby Selma Hurem Leonardo Martín Martín Dag Anders Brede Hans Christian Teien Deborah H Oughton Brit Salbu Jan Ludvig Lyche Peter Aleström 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0212123 https://doaj.org/article/4a1ddb464dd9409cad2c7250dc601439 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0212123 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0212123 https://doaj.org/article/4a1ddb464dd9409cad2c7250dc601439 PLoS ONE, Vol 14, Iss 2, p e0212123 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1371/journal.pone.0212123 2022-12-31T07:07:00Z Ionizing radiation is a recognized genotoxic agent, however, little is known about the role of the functional form of DNA in these processes. Post translational modifications on histone proteins control the organization of chromatin and hence control transcriptional responses that ultimately affect the phenotype. The purpose of this study was to investigate effects on chromatin caused by ionizing radiation in fish. Direct exposure of zebrafish (Danio rerio) embryos to gamma radiation (10.9 mGy/h for 3h) induced hyper-enrichment of H3K4me3 at the genes hnf4a, gmnn and vegfab. A similar relative hyper-enrichment was seen at the hnf4a loci of irradiated Atlantic salmon (Salmo salar) embryos (30 mGy/h for 10 days). At the selected genes in ovaries of adult zebrafish irradiated during gametogenesis (8.7 and 53 mGy/h for 27 days), a reduced enrichment of H3K4me3 was observed, which was correlated with reduced levels of histone H3 was observed. F1 embryos of the exposed parents showed hyper-methylation of H3K4me3, H3K9me3 and H3K27me3 on the same three loci, while these differences were almost negligible in F2 embryos. Our results from three selected loci suggest that ionizing radiation can affect chromatin structure and organization, and that these changes can be detected in F1 offspring, but not in subsequent generations. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles PLOS ONE 14 2 e0212123 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Leif Christopher Lindeman Jorke Harmen Kamstra Jarle Ballangby Selma Hurem Leonardo Martín Martín Dag Anders Brede Hans Christian Teien Deborah H Oughton Brit Salbu Jan Ludvig Lyche Peter Aleström Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon. |
topic_facet |
Medicine R Science Q |
description |
Ionizing radiation is a recognized genotoxic agent, however, little is known about the role of the functional form of DNA in these processes. Post translational modifications on histone proteins control the organization of chromatin and hence control transcriptional responses that ultimately affect the phenotype. The purpose of this study was to investigate effects on chromatin caused by ionizing radiation in fish. Direct exposure of zebrafish (Danio rerio) embryos to gamma radiation (10.9 mGy/h for 3h) induced hyper-enrichment of H3K4me3 at the genes hnf4a, gmnn and vegfab. A similar relative hyper-enrichment was seen at the hnf4a loci of irradiated Atlantic salmon (Salmo salar) embryos (30 mGy/h for 10 days). At the selected genes in ovaries of adult zebrafish irradiated during gametogenesis (8.7 and 53 mGy/h for 27 days), a reduced enrichment of H3K4me3 was observed, which was correlated with reduced levels of histone H3 was observed. F1 embryos of the exposed parents showed hyper-methylation of H3K4me3, H3K9me3 and H3K27me3 on the same three loci, while these differences were almost negligible in F2 embryos. Our results from three selected loci suggest that ionizing radiation can affect chromatin structure and organization, and that these changes can be detected in F1 offspring, but not in subsequent generations. |
format |
Article in Journal/Newspaper |
author |
Leif Christopher Lindeman Jorke Harmen Kamstra Jarle Ballangby Selma Hurem Leonardo Martín Martín Dag Anders Brede Hans Christian Teien Deborah H Oughton Brit Salbu Jan Ludvig Lyche Peter Aleström |
author_facet |
Leif Christopher Lindeman Jorke Harmen Kamstra Jarle Ballangby Selma Hurem Leonardo Martín Martín Dag Anders Brede Hans Christian Teien Deborah H Oughton Brit Salbu Jan Ludvig Lyche Peter Aleström |
author_sort |
Leif Christopher Lindeman |
title |
Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon. |
title_short |
Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon. |
title_full |
Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon. |
title_fullStr |
Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon. |
title_full_unstemmed |
Gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and Atlantic salmon. |
title_sort |
gamma radiation induces locus specific changes to histone modification enrichment in zebrafish and atlantic salmon. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pone.0212123 https://doaj.org/article/4a1ddb464dd9409cad2c7250dc601439 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE, Vol 14, Iss 2, p e0212123 (2019) |
op_relation |
https://doi.org/10.1371/journal.pone.0212123 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0212123 https://doaj.org/article/4a1ddb464dd9409cad2c7250dc601439 |
op_doi |
https://doi.org/10.1371/journal.pone.0212123 |
container_title |
PLOS ONE |
container_volume |
14 |
container_issue |
2 |
container_start_page |
e0212123 |
_version_ |
1766361905161568256 |