Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics
Marine forests and kelps as their foundation species are threatened by ocean warming especially at the warm distributional edges. Previously identified genetic divergence and ecotypic differentiation within kelp species may allow to produce more resilient lineages by intraspecific outbreeding among...
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2022
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Online Access: | https://doi.org/10.3389/fmars.2022.838793 https://doaj.org/article/49dd08c2b7ca4804875f6a706b63695c |
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ftdoajarticles:oai:doaj.org/article:49dd08c2b7ca4804875f6a706b63695c 2023-05-15T14:56:48+02:00 Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics Daniel Liesner Gareth A. Pearson Inka Bartsch Shivani Rana Lars Harms Sandra Heinrich Kai Bischof Gernot Glöckner Klaus Valentin 2022-03-01T00:00:00Z https://doi.org/10.3389/fmars.2022.838793 https://doaj.org/article/49dd08c2b7ca4804875f6a706b63695c EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2022.838793/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.838793 https://doaj.org/article/49dd08c2b7ca4804875f6a706b63695c Frontiers in Marine Science, Vol 9 (2022) temperature performance marine forest genotype inbreeding outbreeding gene expression Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2022 ftdoajarticles https://doi.org/10.3389/fmars.2022.838793 2022-12-31T12:45:20Z Marine forests and kelps as their foundation species are threatened by ocean warming especially at the warm distributional edges. Previously identified genetic divergence and ecotypic differentiation within kelp species may allow to produce more resilient lineages by intraspecific outbreeding among populations. In a mechanistic investigation of heat stress, heterosis (hybrid vigour), and underlying gene expression patterns, we assessed the thermal performance of inbred (selfings) and outbred (reciprocal crosses) sporophytes of the N-Atlantic kelp Laminaria digitata among clonal isolates from two divergent populations; one from the temperate North Sea (Helgoland) and one from the Arctic (Spitsbergen). First, we investigated the upper thermal tolerance of microscopic sporophytes in a 14-day experiment applying sublethal to lethal 20–23°C. The upper survival temperature of microscopic sporophytes was lower for the inbred Arctic selfing (21°C) than for the temperate selfing and the reciprocal crosses (22°C). Only in the temperate selfing, 4.5% of sporophytes survived 23°C. We then subjected 4–7 cm long sporophytes to a control temperature (10°C), moderate (19°C) and sublethal to lethal heat stress (20.5°C) for 18 days to assess gene expression in addition to physiological parameters. Growth and optimum quantum yield decreased similarly in the reciprocal crosses and the temperate selfing at 19 and 20.5°C, while inbred Arctic sporophytes died within seven days at both 19 and 20.5°C. In response to 20.5°C, 252 genes were constitutively regulated across all surviving lineages, which we use to describe metabolic regulation patterns in response to heat stress in kelp. At sublethal 20.5°C, ca. 150 genes were differentially expressed by either crossed lineage in comparison to the temperate selfing, indicating that they maintained a growth response similar to the temperate selfing with differential metabolic regulation during sublethal heat stress. Subtle differences in physiology and the differential expression of nine ... Article in Journal/Newspaper Arctic Spitsbergen Directory of Open Access Journals: DOAJ Articles Arctic Helgoland Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
temperature performance marine forest genotype inbreeding outbreeding gene expression Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
temperature performance marine forest genotype inbreeding outbreeding gene expression Science Q General. Including nature conservation geographical distribution QH1-199.5 Daniel Liesner Gareth A. Pearson Inka Bartsch Shivani Rana Lars Harms Sandra Heinrich Kai Bischof Gernot Glöckner Klaus Valentin Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics |
topic_facet |
temperature performance marine forest genotype inbreeding outbreeding gene expression Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Marine forests and kelps as their foundation species are threatened by ocean warming especially at the warm distributional edges. Previously identified genetic divergence and ecotypic differentiation within kelp species may allow to produce more resilient lineages by intraspecific outbreeding among populations. In a mechanistic investigation of heat stress, heterosis (hybrid vigour), and underlying gene expression patterns, we assessed the thermal performance of inbred (selfings) and outbred (reciprocal crosses) sporophytes of the N-Atlantic kelp Laminaria digitata among clonal isolates from two divergent populations; one from the temperate North Sea (Helgoland) and one from the Arctic (Spitsbergen). First, we investigated the upper thermal tolerance of microscopic sporophytes in a 14-day experiment applying sublethal to lethal 20–23°C. The upper survival temperature of microscopic sporophytes was lower for the inbred Arctic selfing (21°C) than for the temperate selfing and the reciprocal crosses (22°C). Only in the temperate selfing, 4.5% of sporophytes survived 23°C. We then subjected 4–7 cm long sporophytes to a control temperature (10°C), moderate (19°C) and sublethal to lethal heat stress (20.5°C) for 18 days to assess gene expression in addition to physiological parameters. Growth and optimum quantum yield decreased similarly in the reciprocal crosses and the temperate selfing at 19 and 20.5°C, while inbred Arctic sporophytes died within seven days at both 19 and 20.5°C. In response to 20.5°C, 252 genes were constitutively regulated across all surviving lineages, which we use to describe metabolic regulation patterns in response to heat stress in kelp. At sublethal 20.5°C, ca. 150 genes were differentially expressed by either crossed lineage in comparison to the temperate selfing, indicating that they maintained a growth response similar to the temperate selfing with differential metabolic regulation during sublethal heat stress. Subtle differences in physiology and the differential expression of nine ... |
format |
Article in Journal/Newspaper |
author |
Daniel Liesner Gareth A. Pearson Inka Bartsch Shivani Rana Lars Harms Sandra Heinrich Kai Bischof Gernot Glöckner Klaus Valentin |
author_facet |
Daniel Liesner Gareth A. Pearson Inka Bartsch Shivani Rana Lars Harms Sandra Heinrich Kai Bischof Gernot Glöckner Klaus Valentin |
author_sort |
Daniel Liesner |
title |
Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics |
title_short |
Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics |
title_full |
Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics |
title_fullStr |
Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics |
title_full_unstemmed |
Increased Heat Resilience of Intraspecific Outbred Compared to Inbred Lineages in the Kelp Laminaria digitata: Physiology and Transcriptomics |
title_sort |
increased heat resilience of intraspecific outbred compared to inbred lineages in the kelp laminaria digitata: physiology and transcriptomics |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.838793 https://doaj.org/article/49dd08c2b7ca4804875f6a706b63695c |
geographic |
Arctic Helgoland |
geographic_facet |
Arctic Helgoland |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_source |
Frontiers in Marine Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2022.838793/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.838793 https://doaj.org/article/49dd08c2b7ca4804875f6a706b63695c |
op_doi |
https://doi.org/10.3389/fmars.2022.838793 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
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1766328870453116928 |