Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters

• Published in: Marine Drugs
• Main Authors: Magnús Örn Stefánsson, Sigurður Baldursson, Kristinn P. Magnússon, Arnheiður Eyþórsdóttir, Hjörleifur Einarsson
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2019
• Subjects: single cell oil; biomass; PUFA; docosahexaenoic acid (DHA); fish byproducts; biodiesel; Biology (General); QH301-705.5; Eyjafjörður; Skagaströnd; Hveravík; Iceland
• Online Access: https://doi.org/10.3390/md17080449; https://doaj.org/article/49ce07fad63040978e4dd6e2dd5d4191

The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A ( <math display="inline"> <semantics> <mrow> <mn>2.10</mn> <mo>±</mo> <mn>0.70</mn> </mrow> </semantics> </math> g/L) and B ( <math display="inline"> <semantics> <mrow> <mn>1.54</mn> <mo>±</mo> <mn>0.17</mn> </mrow> </semantics> </math> g/L) produced more biomass than the ones isolated from Location C ( <math display="inline"> <semantics> <mrow> <mn>0.43</mn> <mo>±</mo> <mn>0.02</mn> </mrow> </semantics> </math> g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine ( <math display="inline"> <semantics> <mrow> <mn>2.55</mn> <mo>±</mo> <mn>0.74</mn> </mrow> </semantics> </math> g/L) as compared to of commercial origin ( <math display="inline"> <semantics> <mrow> <mn>1.06</mn> <mo>±</mo> <mn>0.57</mn> </mrow> </semantics> </math> g/L). Glycerol ( <math display="inline"> <semantics> <mrow> <mn>2.43</mn> <mo>±</mo> <mn>0.56</mn> </mrow> </semantics> </math> g/L) was a ...